Clemens Glaubitz
Goethe University Frankfurt
Solid-state nuclear magnetic resonanceChemistryBiochemistryNuclear magnetic resonance spectroscopyCrystallography
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Publications 158
#1Nina Kubatova (Goethe University Frankfurt)H-Index: 4
#2Jiafei Mao (Goethe University Frankfurt)H-Index: 5
Last. Harald Schwalbe (Goethe University Frankfurt)H-Index: 55
view all 8 authors...
The RHO gene encodes the GPCR rhodopsin. Numerous mutations associated with impaired visual cycle have been reported; the G90D mutation leads to a constitutively active mutant form of rhodopsin that causes CSNB disease. We report here on the structure investigation of the retinal configuration and conformation in the binding pocket in the dark and light-activated state by solution and MAS-NMR spectroscopy. We find two long-lived dark states for the G90D mutant with the 11-cis retinal bound as Sc...
#1Julian de Mos (Goethe University Frankfurt)H-Index: 1
#2Andreas Jakob (Goethe University Frankfurt)H-Index: 2
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 33
view all 5 authors...
Light-induced activation of biomolecules by uncaging of photolabile protection groups has found many applications for triggering biochemical reactions with minimal perturbations directly within cells. Such an approach might also offer unique advantages for solid-state NMR experiments on membrane proteins when initiating reactions within or at the membrane directly within the closed MAS rotor. Here, we demonstrate that the integral membrane protein E. coli diacylglycerol kinase (DgkA), which cata...
#1Daniel J. Price (Goethe University Frankfurt)H-Index: 5
#2Anita Nair (Merck KGaA)H-Index: 4
Last. Christoph Saal (Merck KGaA)H-Index: 9
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Abstract Mesoporous silica has emerged as an enabling formulation for poorly soluble active pharmaceutical ingredients (APIs). Unlike other formulations, mesoporous silica typically does not inhibit precipitation of supersaturated API therefore, a suitable precipitation inhibitor (PI) should be added to increase absorption from the gastrointestinal (GI) tract. However, there is limited research about optimal processes for combining PIs with silica formulations. Typically, the PI is added by simp...
#1Jiafei Mao (Goethe University Frankfurt)H-Index: 5
#2Victoria Aladin (University of Rostock)H-Index: 3
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 33
view all 9 authors...
Although the rapid development of sensitivity-enhanced solid-state NMR (ssNMR) spectroscopy based on dynamic nuclear polarization (DNP) has enabled a broad range of novel applications in material and life sciences, further methodological improvements are needed to unleash the full potential of DNP-ssNMR. Here, a new methyl-based toolkit for exploring protein structures is presented, which combines signal-enhancement by DNP with heteronuclear Overhauser effect (hetNOE), carbon–carbon-spin diffusi...
1 CitationsSource
#1Felix Ditzinger (University of Basel)H-Index: 3
#2Daniel J. Price (Merck KGaA)H-Index: 5
Last. Martin Kuentz (University of Applied Sciences and Arts Northwestern Switzerland FHNW)H-Index: 18
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Amorphous formulation technologies to improve oral absorption of poorly soluble active pharmaceutical ingredients (APIs) have become increasingly prevalent. Currently, polymer-based amorphous formulations manufactured by spray drying, hot melt extrusion (HME), or co-precipitation are most common. However, these technologies have challenges in terms of the successful stabilization of poor glass former compounds in the amorphous form. An alternative approach is mesoporous silica, which stabilizes ...
#1Jakob Maciejko (Goethe University Frankfurt)H-Index: 2
#2Jagdeep Kaur (Goethe University Frankfurt)H-Index: 4
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 33
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Proteorhodopsin (PR) is a highly abundant, pentameric, light-driven proton pump. Proton transfer is linked to a canonical photocycle typical for microbial ion pumps. Although the PR monomer is able to undergo a full photocycle, the question arises whether the pentameric complex formed in the membrane via specific cross-protomer interactions plays a role in its functional mechanism. Here, we use dynamic nuclear polarization (DNP)-enhanced solid-state magic-angle spinning (MAS) NMR in combination ...
2 CitationsSource
#1Jagdeep Kaur (Goethe University Frankfurt)H-Index: 4
#2Clara Nassrin Kriebel (Goethe University Frankfurt)H-Index: 2
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 33
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Abstract Krokinobacter eikastus rhodopsin 2 (KR2) is a pentameric, light-driven ion pump, which selectively transports sodium or protons. The mechanism of ion selectivity and transfer is unknown. By using conventional as well as dynamic nuclear polarization (DNP)-enhanced solid-state NMR, we were able to analyse the retinal polyene chain between positions C10 and C15 as well as the Schiff base nitrogen in the KR2 resting state. In addition, 50% of the KR2 13C and 15N resonances could be assigned...
9 CitationsSource
#1Kristin Möbius (Goethe University Frankfurt)H-Index: 1
#2Sina Kazemi (Goethe University Frankfurt)H-Index: 5
Last. Clemens Glaubitz (Goethe University Frankfurt)H-Index: 33
view all 7 authors...
Escherichia coli diacylglycerol kinase (DGK) is an integral membrane protein, which catalyses the ATP-dependent phosphorylation of diacylglycerol (DAG) to phosphatic acid (PA). It is a unique trimeric enzyme, which does not share sequence homology with typical kinases. It exhibits a notable complexity in structure and function despite of its small size. Here, chemical shift assignment of wild-type DGK within lipid bilayers was carried out based on 3D MAS NMR, utilizing manual and automatic analy...
2 CitationsSource
#1Marvin Asido (Goethe University Frankfurt)H-Index: 1
#2Peter Eberhardt (Goethe University Frankfurt)H-Index: 3
Last. Josef Wachtveitl (Goethe University Frankfurt)H-Index: 38
view all 6 authors...
We report a comparative study on the structural dynamics of the light-driven sodium pump Krokinobacter eikastus rhodopsin 2 wild type under sodium and proton pumping conditions by means of time-resolved IR spectroscopy. The kinetics of KR2 under sodium pumping conditions exhibits a sequential character, whereas the kinetics of KR2 under proton pumping conditions involves several equilibrium states. The sodium translocation itself is characterized by major conformational changes of the protein ba...
2 CitationsSource
#1Dimitrios Ntountaniotis (UoA: National and Kapodistrian University of Athens)H-Index: 8
#2Ioannis Andreadelis (UoA: National and Kapodistrian University of Athens)H-Index: 2
Last. Thomas Mavromoustakos (UoA: National and Kapodistrian University of Athens)H-Index: 33
view all 23 authors...
Renin–angiotensin aldosterone system inhibitors are for a long time extensively used for the treatment of cardiovascular and renal diseases. AT1 receptor blockers (ARBs or sartans) act as antihypertensive drugs by blocking the octapeptide hormone Angiotensin II to stimulate AT1 receptors. The antihypertensive drug candesartan (CAN) is the active metabolite of candesartan cilexetil (Atacand, CC). Complexes of candesartan and candesartan cilexetil with 2-hydroxylpropyl-β-cyclodextrin (2-HP-β-CD) w...
2 CitationsSource