Match!
Webe Kadima
University of California, Riverside
4Publications
4H-index
128Citations
Publications 4
Newest
The role of metal ions in the T- to R-allosteric transition is ascertained from the investigation of the T- to R-allosteric transition of transition metal ions substituted−insulin hexamers, as well as from the kinetics of their dissociation. These studies establish that ligand field stabilization energy (LFSE), coordination geometry preference, and the Lewis acidity of the metal ion in the zinc sites modulate the T- to R-state transition. 1H NMR, 113Cd NMR, and UV−vis measurements demonstrate th...
#1Webe Kadima (UCR: University of California, Riverside)H-Index: 4
#2Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
Last.Frances Jurnak (UCR: University of California, Riverside)H-Index: 25
view all 4 authors...
Abstract The aggregation of insulin prior to crystallization as a rhombohedral crystal of the 2Zn-insulin hexamer was studied by dynamic light scattering (DLS) using photon correlation spectroscopy (PCS). Results indicate that the 2Zn-insulin hexamer does not aggregate into higher oligomers in solution prior to crystallization. It is, therefore, suggested that the hexamer is the crystallizing species, strongly implying that the crystal grows by addition of hexamers to the crystal. This behavior ...
#1Webe Kadima (UCR: University of California, Riverside)H-Index: 4
#2Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
Last.Frances Jurnak (UCR: University of California, Riverside)H-Index: 25
view all 4 authors...
The aggregation processes leading to crystallization and precipitation of canavalin have been investigated by dynamic light scattering (DLS) in photon correlation spectroscopy (PCS) mode. The sizes of aggregates formed under various conditions of pH, salt concentration, and protein concentrations were deduced from the correlation functions generated by the fluctuating intensity of light scattered by the solutions of the protein. Results obtained indicate that the barrier to crystallization of ca...
1