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Yong Hwan Lee
Louisiana State University
1Publications
1H-index
9Citations
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#1Tae Woong Whon (Korea Research Institute of Bioscience and Biotechnology)H-Index: 1
#2Yong Hwan Lee (LSU: Louisiana State University)H-Index: 1
Last.Song G. Kim (Korea Research Institute of Bioscience and Biotechnology)H-Index: 1
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A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the `upside-down sitting-drop' method could produce single crystals with all the benefits of the hanging-...
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