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Yukiko Yamashita
University of Tokushima
2Publications
2H-index
93Citations
Publications 2
Newest
#1Masakazu Hashimoto (Osaka University)H-Index: 7
#2Yukiko Yamashita (University of Tokushima)H-Index: 2
Last.Tatsuya Takemoto (University of Tokushima)H-Index: 13
view all 3 authors...
The CRISPR/Cas9 system is a powerful tool for elucidating the roles of genes in a wide variety of organisms including mice. To obtain genetically modified embryos or mice by this method, Cas9 mRNA and sgRNA are usually introduced into zygotes by microinjection or electroporation. However, most mutants generated with this method are genetically mosaic, composed of several types of cells carrying different mutations, which complicates phenotype analysis in founder embryos or mice. To simplify the ...
65 CitationsSource
#1Fuminori Tanihara (University of Tokushima)H-Index: 9
#2Tatsuya Takemoto (University of Tokushima)H-Index: 13
Last.Takeshige Otoi (University of Tokushima)H-Index: 30
view all 19 authors...
Genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer technique; however, this approach requires complex micromanipulation techniques and sometimes increases the risks of both prenatal and postnatal death by faulty epigenetic reprogramming of a donor somatic cell nucleus. As a result, the production of genetically modified pigs has not been widely applied. We provide a simple method for CRISPR (clustered regularly interspaced sho...
28 CitationsSource
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