Xiang Zheng
Tsinghua University
Publications 4
#1Zheng Lin Tan (THU: Tsinghua University)
#2Xiang Zheng (THU: Tsinghua University)H-Index: 3
Last.Chong Zhang (THU: Tsinghua University)H-Index: 24
view all 6 authors...
Microorganisms have long been used as chemical plant to convert simple substrates into complex molecules. Various metabolic pathways have been optimised over the past few decades, but the progresses were limited due to our finite knowledge on metabolism. Evolution is a knowledge-free genetic randomisation approach, employed to improve the chemical production in microbial cell factories. However, evolution of large, complex pathway was a great challenge. The invention of continuous culturing syst...
#1Xiang Zheng (THU: Tsinghua University)H-Index: 3
#2Xin-Hui Xing (THU: Tsinghua University)H-Index: 27
Last.Chong Zhang (THU: Tsinghua University)H-Index: 24
view all 3 authors...
Abstract Mutations, serving as the raw materials of evolution, have been extensively utilized to increase the chances of engineering molecules or microbes with tailor-made functions. Global and targeted mutagenesis are two main methods of obtaining various mutations, distinguished by the range of action they can cover. While the former one stresses the mining of novel genetic loci within the whole genomic background, targeted mutagenesis performs in a more straightforward manner, bringing evolut...
8 CitationsSource
#1Tong-Xin Zhao (THU: Tsinghua University)H-Index: 2
#2Mei Li (THU: Tsinghua University)H-Index: 2
Last.Xin-Hui Xing (THU: Tsinghua University)H-Index: 27
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trans-4-Hydroxy-l-proline (Hyp) is a chiral amino acid conventionally produced by acid hydrolysis of animal collagen, a process which involves the bottleneck problems of low efficiency and heavy environmental pollution. Biotransformation of l-proline into Hyp using recombinant whole-cell biocatalysis with proline-4-hydroxylase (P4H) is an environmentally-friendly alternative method. Since biohydroxylation of proline by whole cells is a high-oxygen-demand process, oxygen transfer needs to be impr...
6 CitationsSource
#1Ming-Yue Fang (THU: Tsinghua University)H-Index: 5
#2Tianmin Wang (THU: Tsinghua University)H-Index: 7
Last.Xin-Hui Xing (THU: Tsinghua University)H-Index: 27
view all 8 authors...
Abstract Because high-throughput screening tools are typically unavailable when using the pathway-engineering approach, we developed a new strategy, named intermediate sensor-assisted push–pull strategy, which enables sequential pathway optimization by incorporating a biosensor targeting a key pathway intermediate. As proof of concept, we constructed an l -Trp biosensor and used it to optimize the deoxyviolacein biosynthetic pathway, which we divided into two modules with l -Trp being the produc...
29 CitationsSource