Match!
Vincent Mikol
Centre national de la recherche scientifique
8Publications
7H-index
288Citations
Publications 8
Newest
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Pascale Vincendon (CNRS: Centre national de la recherche scientifique)H-Index: 6
Last.Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 5 authors...
Abstract The apparent hydrodynamic radius of a truncated form of baker's yeast aspartyl-tRNA synthetase has been measured in various precipitating agent solutions as a function of the protein concentration by dynamic light scattering. In solvents containing ammonium sulfate or 2-methyl-2,4-pentanediol as the precipitating agent the protein remains essentially monodisperse, whereas in the presence of polyethylene glycol interactions and aggregations between protein molecules are detected before r...
22 CitationsSource
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Ernest HirschH-Index: 6
Last.Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 3 authors...
The translational diffusion coefficient D 25,w of hen egg-white lysozyme and concanavalin A from the jack bean is measured in various precipitating agent solutions as a function of salt and protein concentration using quasi-elastic light-scattering. With some precipitants, in undersaturated protein solutions, a protein or salt concentration dependence of the diffision coefficient of the scatterers is observed. It can be correlated with the inability of the protein to crystallize in this precipit...
106 CitationsSource
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Jean-Luc Rodeau (CNRS: Centre national de la recherche scientifique)H-Index: 16
Last.Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 3 authors...
Abstract The hanging drop method for protein crystallization consists of equilibrating a water droplet containing the protein and a precipitant against a reservoir solution at a higher precipitant concentration. The time for water equilibration—which affects the kinetics of crystallization—to reach 90% of completion is shown to vary between about 25 h and more than 25 days, depending on experimental conditions. Experiments were performed with three of the most widely used precipitants (ammonium ...
31 CitationsSource
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Jean-Luc Rodeau (CNRS: Centre national de la recherche scientifique)H-Index: 16
Last.Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 4 authors...
Until recently crystallization of macromolecules was rather empirical. Stimulated by developments of biotechnology and by the crystallization programs under microgravity, this empirical field is moving towards the new discipline of biological crystallogenesis, which includes biology, physics, crystallography and related engineering aspects. Here we will discuss attempts to control better the physical basis of the crystallization of biological macromolecules and to set up in a rational way crysta...
2 CitationsSource
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Ernest HirschH-Index: 6
Last.Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 3 authors...
Crystallization of lysozymes induced by temperature lowering has been monitored by dynamic light scattering from the onset of supersaturation to the growth of protein crystals up to a noticeable size (150–200 μm). The apparent size of the scatterers was found to increase up to a maximum value as supersaturation proceeded, then to decrease down to its initial value. Apparitions of crystals (20–30 μm) were observed during this decrease. Light scattering is thus proved to be a sensitive technique t...
35 CitationsSource
#1Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
#2Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
Abstract Until recently, crystallization of macromolecules was rather an empirical process. Developments in biotechnology have emphasized the need for a more rational approach. Indeed a new discipline, biological crystallogenesis, encompassing biology, physics, crystallography and related engineering aspects, is emerging. This article discusses theoretical and new methodological principles underlying biological crystallogenesis. It gives special emphasis to the concept of purity of macromolecule...
17 CitationsSource
#1Vincent Mikol (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
Abstract A quick and miniature method has been devised for determining protein solubility and used to investigate the equilibrium solubility of concanavalin A from the Jack Bean with its crystals as a function of ammonium sulfate concentration, temperature and pH. The crystals were characterized by X-ray diffraction and their morphologies related to the corresponding solubilities. The protein solution concentration was estimated out of small crystallizing drops using a rapid and sensitive microa...
45 CitationsSource
#1Vincent MikolH-Index: 7
#2Jean-Luc RodeauH-Index: 16
Last.Richard GiegéH-Index: 54
view all 3 authors...
Possible pH variations during crystallization of biological macromolecules by the vapor diffusion method have not been taken into account in most experiments so far reported. The present study demonstrates that when ammonium sulfate is used as the precipitant, pH changes occur due to ammonia transfer following ammonium/ammonia equilibrium. The pH in a crystallization droplet is shown to be controlled by that of the reservoir. The theory of the effect is given and the consequences of pH variation...
30 CitationsSource
1