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Marieke Aarts
Netherlands Cancer Institute
7Publications
7H-index
190Citations
Publications 7
Newest
#1Marleen Dekker (NKI-AVL: Netherlands Cancer Institute)H-Index: 12
#2Sandra S. de Vries (NKI-AVL: Netherlands Cancer Institute)H-Index: 10
Last.Hein te Riele (NKI-AVL: Netherlands Cancer Institute)H-Index: 29
view all 10 authors...
Abstract Short synthetic single-stranded oligodeoxyribonucleotides (ssODNs) can be used to introduce subtle modifications into the genome of mouse embryonic stem cells (ESCs). We have previously shown that effective application of ssODN-mediated gene targeting in ESC requires (transient) suppression of DNA mismatch repair (MMR). However, whereas transient down-regulation of the mismatch recognition protein MSH2 allowed substitution of 3 or 4 nucleotides, 1 or 2 nucleotide substitutions were stil...
15 CitationsSource
#1Marieke Aarts (NKI-AVL: Netherlands Cancer Institute)H-Index: 7
#2H. te Riele (NKI-AVL: Netherlands Cancer Institute)H-Index: 6
Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is a promising technique for introducing site-specific sequence alterations without affecting the genomic organization of the target locus. Here, we discuss the significant progress that has been made over the last 5 years in unraveling the mechanisms and reaction parameters underlying ssODN-mediated gene targeting. We will specifically focus on ssODN-mediated gene targeting in murine embryonic stem cells (ESCs) and the impact ...
41 CitationsSource
#1Marieke Aarts (NKI-AVL: Netherlands Cancer Institute)H-Index: 7
#2Hein te Riele (NKI-AVL: Netherlands Cancer Institute)H-Index: 29
Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is a promising tool for site-specific gene modification in mouse embryonic stem cells (ESCs). We have developed an ESC line carrying a mutant EGFP reporter gene to monitor gene correction events shortly after exposure to ssODNs. We used this system to compare the appearance and fate of cells corrected by sense or anti-sense ssODNs. The slower appearance of green fluorescent cells with sense ssODNs as compared to anti-sense ssOD...
26 CitationsSource
#1Marieke Aarts (NKI-AVL: Netherlands Cancer Institute)H-Index: 7
#2Hein te Riele (NKI-AVL: Netherlands Cancer Institute)H-Index: 29
Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is emerging as a powerful tool for the introduction of subtle gene modifications in mouse embryonic stem (ES) cells and the generation of mutant mice. Here, we have studied the role of ssODN composition, transcription and replication of the target locus, and DNA repair pathways to gain more insight into the parameters governing ssODN-mediated gene targeting in mouse ES cells. We demonstrated that unmodified ssODNs of 35–40 nt w...
16 CitationsSource
#1Marieke Aarts (NKI-AVL: Netherlands Cancer Institute)H-Index: 7
#2Marleen Dekker (NKI-AVL: Netherlands Cancer Institute)H-Index: 12
Last.Hein te Riele (NKI-AVL: Netherlands Cancer Institute)H-Index: 29
view all 7 authors...
8 CitationsSource
#1Marieke AartsH-Index: 7
#2Marleen DekkerH-Index: 12
Last.Hein te RieleH-Index: 13
view all 5 authors...
Oligonucleotide-mediated gene targeting is emerging as a powerful tool for the introduction of subtle gene modifications in mouse embryonic stem (ES) cells and the generation of mutant mice. However, its efficacy is strongly suppressed by DNA mismatch repair (MMR). Here we report a simple and rapid procedure for the generation of mouse mutants using transient down regulation of the central MMR protein MSH2 by RNA interference. We demonstrate that under this condition, unmodified single-stranded ...
49 CitationsSource
#1Marleen Dekker (NKI-AVL: Netherlands Cancer Institute)H-Index: 12
#2Conny Brouwers (NKI-AVL: Netherlands Cancer Institute)H-Index: 4
Last.H. te Riele (NKI-AVL: Netherlands Cancer Institute)H-Index: 6
view all 7 authors...
We have previously demonstrated that site-specific insertion, deletion or substitution of one or two nucleotides in mouse embryonic stem cells (ES cells) by single-stranded deoxyribo-oligonucleotides is several hundred-fold suppressed by DNA mismatch repair (MMR) activity. Here, we have investigated whether compound mismatches and larger insertions escape detection by the MMR machinery and can be effectively introduced in MMR-proficient cells. We identified several compound mismatches that escap...
35 CitationsSource
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