Daochun Kong
Peking University
Control of chromosome duplicationOrigin recognition complexMolecular biologyDNA replicationBiology
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Publications 20
#1Gang Feng (PKU: Peking University)
#2Yue Yuan (PKU: Peking University)
Last. Daochun Kong (PKU: Peking University)H-Index: 13
view all 8 authors...
DNA replication forks in eukaryotic cells stall at a variety of replication barriers. Stalling forks require strict cellular regulations to prevent fork collapse. However, the mechanism underlying these cellular regulations is poorly understood. In this study, a cellular mechanism was uncovered that regulates chromatin structures to stabilize stalling forks. When replication forks stall, H2BK33, a newly identified acetylation site, is deacetylated and H3K9 trimethylated in the nucleosomes surrou...
#1Lihong Wu (PKU: Peking University)
#2Yu Hua (PKU: Peking University)
Last. Daochun Kong (PKU: Peking University)H-Index: 13
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A central event in the initiation of DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) on specific chromatin sites known as DNA replication origins. The pre-RC assembly process differs between budding and fission yeasts. In fission yeast, Sap1 directly participates in pre-RC assembly, together with the four initiation factors: ORC, Cdc18/Cdc6, Cdt1, and MCM. In metazoans, the nature of DNA replication origins is not defined and the mechanism of pre-RC assembly rem...
#1Ling Guan (THU: Tsinghua University)H-Index: 1
#2Peng He (THU: Tsinghua University)H-Index: 1
Last. Daochun Kong (THU: Tsinghua University)H-Index: 13
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3 CitationsSource
#1Bochao Liu (PKU: Peking University)H-Index: 1
#2Jiazhi Hu (PKU: Peking University)H-Index: 12
Last. Daochun Kong (PKU: Peking University)H-Index: 13
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Abstract During DNA replication in eukaryotic cells, short single-stranded DNA segments known as Okazaki fragments are first synthesized on the lagging strand. The Okazaki fragments originate from ∼35-nucleotide-long RNA-DNA primers. After Okazaki fragment synthesis, these primers must be removed to allow fragment joining into a continuous lagging strand. To date, the models of enzymatic machinery that removes the RNA-DNA primers have come almost exclusively from biochemical reconstitution studi...
14 CitationsSource
#1Huimin Zhang (PKU: Peking University)H-Index: 1
#2Yu Hua (PKU: Peking University)H-Index: 3
Last. Daochun Kong (PKU: Peking University)H-Index: 13
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Abstract Double-stranded DNA breaks (DSBs) are highly detrimental DNA lesions, which may be repaired by the homologous recombination-mediated repair pathway. The 5 prime to 3 prime direction of long-range end resection on one DNA strand, in which 3 prime-single-strand DNA overhangs are created from broken DNA ends, is an essential step in this pathway. Dna2 has been demonstrated as an essential nuclease in this event, but the molecular mechanism how Dna2 is recruited to DNA break sites in vivo i...
4 CitationsSource
#1Ming Zeng (Sichuan University)H-Index: 4
#2Laifeng Ren (Shanxi Medical University)H-Index: 7
Last. Cong Liu (Sichuan University)H-Index: 41
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The repair of double-strand DNA breaks by homologous recombination requires resection of the DNA ends. Here the authors show that in Schizosaccharomyces pombe and human cells, Wdr70 is recruited as part of the CRL4 complex to promote ubiquitination of H2B and allow Exo1-mediated resection.
17 CitationsSource
#1LiHong Wu (PKU: Peking University)H-Index: 1
#2Yang Liu (PKU: Peking University)H-Index: 10
Last. Daochun Kong (PKU: Peking University)H-Index: 13
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Chromosomal DNA replication is one of the central biological events occurring inside cells. Due to its large size, the replication of genomic DNA in eukaryotes initiates at hundreds to tens of thousands of sites called DNA origins so that the replication could be completed in a limited time. Further, eukaryotic DNA replication is sophisticatedly regulated, and this regulation guarantees that each origin fires once per S phase and each segment of DNA gets duplication also once per cell cycle. The...
7 CitationsSource
#1Jiazhi Hu (PKU: Peking University)H-Index: 12
#2Lei Sun (CAS: Chinese Academy of Sciences)H-Index: 3
Last. Daochun Kong (PKU: Peking University)H-Index: 13
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SUMMARY When replication forks stall at damaged bases or upon nucleotide depletion, the intra-S phase checkpoint ensures they are stabilized and can restart. In intra-S checkpoint-deficient budding yeast, stalling forks collapse, and � 10% form pathogenic chicken foot structures, contributing to incomplete replication and cell death (Lopes et al., 2001; Sogo et al., 2002; Tercero and Diffley, 2001). Using fission yeast, we report that the Cds1 Chk2 effector kinase targets Dna2 on S220 to regulat...
100 CitationsSource
#1Jingya Sun (ZJOU: Zhejiang Ocean University)H-Index: 1
#2Daochun Kong (PKU: Peking University)H-Index: 13
Chromosomal DNA replication in eukaryotic cells is highly complicated and sophisticatedly regulated. Owing to its large size, a typical eukaryotic genome contains hundreds to tens of thousands of initiation sites called DNA replication origins where DNA synthesis takes place. Multiple initiation sites remove the constraint of a genome size because only a certain amount of DNA can be replicated from a single origin in a limited time. The activation of these multiple origins must be coordinated so...
11 CitationsSource
#1Daochun Kong (NIH: National Institutes of Health)H-Index: 13
#2Thomas Coleman (Kenneth S. Warren Institute)H-Index: 19
Last. Melvin L. DePamphilis (NIH: National Institutes of Health)H-Index: 49
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Budding yeast (Saccharomyces cerevisiae) origin recognition complex (ORC) requires ATP to bind specific DNA sequences, whereas fission yeast (Schizosaccharomyces pombe) ORC binds to specific, asymmetric A:T-rich sites within replication origins, independently of ATP, and frog (Xenopus laevis) ORC seems to bind DNA non-specifically. Here we show that despite these differences, ORCs are functionally conserved. Firstly, SpOrc1, SpOrc4 and SpOrc5, like those from other eukaryotes, bound ATP and exhi...
46 CitationsSource