Hong Ma
Oregon National Primate Research Center
69Publications
27H-index
2,940Citations
Publications 69
Newest
Published on Mar 1, 2019in Nature 41.58
Eunju Kang13
Estimated H-index: 13
(Oregon National Primate Research Center),
Jun Wu28
Estimated H-index: 28
(Salk Institute for Biological Studies)
+ 27 AuthorsYeonmi Lee6
Estimated H-index: 6
(Oregon National Primate Research Center)
Change history In this Letter, there are several errors regarding the assignments of mtDNA haplotypes for a subset of egg donors from our study. These errors have not been corrected online.
Source Cite
Published on Jul 24, 2018in PLOS ONE 2.77
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Yeonmi Lee6
Estimated H-index: 6
(Oregon National Primate Research Center)
+ 12 AuthorsHayley Darby1
Estimated H-index: 1
(Oregon National Primate Research Center)
The accumulation of acquired mitochondrial genome (mtDNA) mutations with aging in somatic cells has been implicated in mitochondrial dysfunction and linked to age-onset diseases in humans. Here, we asked if somatic mtDNA mutations are also associated with aging in the mouse. MtDNA integrity in multiple organs and tissues in young and old (2–34 months) wild type (wt) mice was investigated by whole genome sequencing. Remarkably, no acquired somatic mutations were detected in tested tissues. Howeve...
Source Cite
Ming-Tao Zhao11
Estimated H-index: 11
(Stanford University),
Haodong Chen10
Estimated H-index: 10
(Stanford University)
+ 15 AuthorsYoungkyun Kim8
Estimated H-index: 8
(Stanford University)
Abstract Patient-specific pluripotent stem cells (PSCs) can be generated via nuclear reprogramming by transcription factors (i.e., induced pluripotent stem cells, iPSCs) or by somatic cell nuclear transfer (SCNT). However, abnormalities and preclinical application of differentiated cells generated by different reprogramming mechanisms have yet to be evaluated. Here we investigated the molecular and functional features, and drug response of cardiomyocytes (PSC-CMs) and endothelial cells (PSC-ECs)...
11 Citations Source Cite
Published on Jan 1, 2017in Cell Stem Cell 23.29
Hong Ma27
Estimated H-index: 27
(Oregon Health & Science University),
Ryan C. O’Neil4
Estimated H-index: 4
(University of California, San Diego)
+ 23 AuthorsYeonmi Lee6
Estimated H-index: 6
(Oregon Health & Science University)
Summary Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer of first polar body (PB1) genomes from metaphase II (MII) oocytes into enucleated donor MII cytoplasm (PBNT). The reconstructed oocytes supported the formation of de novo meiotic spindles and, after fertilization with sperm, meiosis completion and ...
18 Citations Source Cite
Published on Jan 1, 2017in Stem Cells 5.59
Don P. Wolf43
Estimated H-index: 43
,
Robert Morey9
Estimated H-index: 9
+ 4 AuthorsShoukhrat Mitalipov36
Estimated H-index: 36
Embryonic stem cells (ESC) hold promise for the treatment of human medical conditions but are allogeneic. Here, we consider the differences between autologous pluripotent stem cells produced by nuclear transfer (NT-ESCs) and transcription factor-mediated, induced pluripotent stem cells (iPSCs) that impact the desirability of each of these cell types for clinical use. The derivation of NT-ESCs is more cumbersome and requires donor oocytes; however, the use of oocyte cytoplasm as the source of rep...
9 Citations Source Cite
Published on Aug 1, 2017in Nature 41.58
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Nuria Marti-Gutierrez1
Estimated H-index: 1
(Oregon Health & Science University)
+ 28 AuthorsRiffat Ahmed10
Estimated H-index: 10
(Oregon Health & Science University)
Genome editing could be applied to correct disease-causing mutations in human embryos, but concerns about efficacy and safety are paramount. Shoukhrat Mitalipov and colleagues use CRISPRCas9 to correct a heritable cardiomyopathy mutation in human embryos. By optimizing the experimental conditions, the authors show very reduced mosaicism, and report that for this heterozygous mutation, CRISPRCas9-induced breaks seem to be preferentially repaired using the wild-type allele as a template in human e...
238 Citations Source Cite
Published on Nov 30, 2016in Nature 41.58
Eunju Kang13
Estimated H-index: 13
(University of Ulsan),
Jun Wu28
Estimated H-index: 28
(Salk Institute for Biological Studies)
+ 27 AuthorsYeonmi Lee6
Estimated H-index: 6
(Oregon Health & Science University)
Analysis of mitochondrial replacement therapy shows, even with efficient mutant mitochondrial DNA replacement and maintenance in embryonic stem cells, a gradual loss of donor mitochondrial DNA in some lines owing to a polymorphism in the D-loop, potentially causing preferential replication of specific mitochondrial DNA haplotypes.
59 Citations Source Cite
Published on May 1, 2016in Cell Stem Cell 23.29
Eunju Kang13
Estimated H-index: 13
(Oregon National Primate Research Center),
Xinjian Wang3
Estimated H-index: 3
(Cincinnati Children's Hospital Medical Center)
+ 20 AuthorsYing Li14
Estimated H-index: 14
(Oregon National Primate Research Center)
Summary The genetic integrity of iPSCs is an important consideration for therapeutic application. In this study, we examine the accumulation of somatic mitochondrial genome (mtDNA) mutations in skin fibroblasts, blood, and iPSCs derived from young and elderly subjects (24–72 years). We found that pooled skin and blood mtDNA contained low heteroplasmic point mutations, but a panel of ten individual iPSC lines from each tissue or clonally expanded fibroblasts carried an elevated load of heteroplas...
70 Citations Source Cite
Published on Nov 11, 2016in Circulation 18.88
Mingtao Zhao (Cardiovascular Institute of the South), Haodong Chen10
Estimated H-index: 10
(Cardiovascular Institute of the South)
+ 10 AuthorsRebecca Tippner-Hedges9
Estimated H-index: 9
(Oregon Health & Science University)
Human pluripotent stem cells (PSCs) hold great promise for disease modeling, drug discovery, and regenerative medicine. Currently, patient-specific PSCs can be created via two approaches: somatic cell nuclear transfer (SCNT) by enucleated oocytes and iPSC reprogramming by ectopic expression of four transcription factors. Recent studies suggest that human SCNT-derived embryonic stem cells (nt-ESCs) are more similar to in vitro fertilized embryo derived ESCs (IVF-ESCs) than iPSCs at both transcrip...
Published on Jun 1, 2016in Current Opinion in Genetics & Development 5.00
Clifford D.L. Folmes16
Estimated H-index: 16
(Mayo Clinic),
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center)
+ 1 AuthorsAndre Terzic74
Estimated H-index: 74
Beyond their canonical role in efficient ATP production through oxidative metabolism, mitochondria are increasingly recognized as critical in defining stem cell function and fate. Implicating a fundamental interplay within the epigenetics of eukaryotic cell systems, the integrity of mitochondria is found vital across the developmental/differentiation spectrum from securing pluripotency maintenance to informing organotypic decisions. This overview will discuss recent progress on examining the pla...
18 Citations Source Cite
1234567