Hui Yang
Chinese Academy of Sciences
Publications 44
#1Xinde Hu (CAS: Chinese Academy of Sciences)H-Index: 9
#2Jinghan Wang (Fudan University)
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 10 authors...
#1Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
Funding research is a challenge faced by most scientists around the world. Genome Biology has invited four scientists based in three different countries to share their own experience and opinions regarding funding, the difficulties young scientists must overcome, and how the process of securing funding can be improved. Here, Hui Yang discusses the funding opportunities open to scientists conducting research in China.
#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
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Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
#1Yajing Liu (CAS: Chinese Academy of Sciences)H-Index: 7
#2Jianan Li (CAS: Chinese Academy of Sciences)
Last.Yunbo Qiao (GU: Guangzhou University)H-Index: 1
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Abstract Allele-specific DNA methylation is the most important imprinting marker localized to differentially methylated regions (DMRs), and aberrant genomic imprinted DNA methylation is associated with some human diseases, including Prader-Willi syndrome and cancer. Thus, the development of an effective strategy for the precise editing of allele-specific methylated genes is essential for the functional clarification of imprinting elements and the correction of imprinting disorders in human disea...
#1Jin-Jing Li (Fujian Medical University)H-Index: 2
#2Xiang Lin (Fujian Medical University)H-Index: 3
Last.Wan-Jin Chen (Fujian Medical University)H-Index: 11
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#1Sanlan Li (CAS: Chinese Academy of Sciences)H-Index: 2
#2Yuhan Shi (CAS: Chinese Academy of Sciences)H-Index: 1
Last.Leping Cheng (CAS: Chinese Academy of Sciences)H-Index: 18
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Summary Dysfunction of noradrenergic (NA) neurons is associated with a number of neuronal disorders. Diverse neuronal subtypes can be generated by direct reprogramming. However, it is still unknown how to convert non-neuronal cells into NA neurons. Here, we show that seven transcription factors (TFs) (Ascl1, Phox2b, AP-2α, Gata3, Hand2, Nurr1, and Phox2a) are able to convert astrocytes and fibroblasts into induced NA (iNA) neurons. These iNA neurons express the genes required for the biosynthesi...
#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last.Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 14 authors...
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4–8, it is now clear that the deaminases that are integral to commonly used DNA base editors often bind to RNA9–13. For example, the cytosine deaminase APOBEC1—which is used...
#1Jie Wang (CAS: Chinese Academy of Sciences)H-Index: 52
#2Sen-Sen Lou (CAS: Chinese Academy of Sciences)
Last.Zhi-Qi Xiong (CAS: Chinese Academy of Sciences)H-Index: 29
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Deficiency in the E3 ubiquitin ligase UBE3A leads to the neurodevelopmental disorder Angelman syndrome (AS), while additional dosage of UBE3A is linked to autism spectrum disorder. The mechanisms underlying the downstream effects of UBE3A gain or loss of function in these neurodevelopmental disorders are still not well understood, and effective treatments are lacking. Here, using stable-isotope labeling of amino acids in mammals and ubiquitination assays, we identify PTPA, an activator of protei...
#1Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
#2Dinshaw J. Patel (SU: Southern University and A&M College)H-Index: 2
#1Haoyi Wang (CAS: Chinese Academy of Sciences)H-Index: 25
#2Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
During the second World Summit of Human Gene Editing, Jiankui He presented the gene-editing project that led to the birth of two baby girls with man-made C-C chemokine receptor type 5 (CCR5) mutations. This extremely irresponsible behavior violated the ethical consensus of scientists all over the world. His presentation revealed a troubling lack not only of basic medical ethics but also of the requisite understanding of genetics and gene editing. Here, we review the rationale and design of his e...