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Hui Yang
Chinese Academy of Sciences
Genome editingMolecular biologyGeneticsBiologyCRISPR
44Publications
19H-index
5,342Citations
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Publications 39
Newest
#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 17 authors...
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
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#1Sanlan Li (CAS: Chinese Academy of Sciences)H-Index: 2
#2Yuhan Shi (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Leping Cheng (CAS: Chinese Academy of Sciences)H-Index: 18
view all 15 authors...
Summary Dysfunction of noradrenergic (NA) neurons is associated with a number of neuronal disorders. Diverse neuronal subtypes can be generated by direct reprogramming. However, it is still unknown how to convert non-neuronal cells into NA neurons. Here, we show that seven transcription factors (TFs) (Ascl1, Phox2b, AP-2α, Gata3, Hand2, Nurr1, and Phox2a) are able to convert astrocytes and fibroblasts into induced NA (iNA) neurons. These iNA neurons express the genes required for the biosynthesi...
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#1Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 14 authors...
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4–8, it is now clear that the deaminases that are integral to commonly used DNA base editors often bind to RNA9–13. For example, the cytosine deaminase APOBEC1—which is used...
16 CitationsSource
#1Jie Wang (CAS: Chinese Academy of Sciences)H-Index: 52
#2Sen-Sen Lou (CAS: Chinese Academy of Sciences)
Last. Zhi-Qi Xiong (CAS: Chinese Academy of Sciences)H-Index: 29
view all 19 authors...
Deficiency in the E3 ubiquitin ligase UBE3A leads to the neurodevelopmental disorder Angelman syndrome (AS), while additional dosage of UBE3A is linked to autism spectrum disorder. The mechanisms underlying the downstream effects of UBE3A gain or loss of function in these neurodevelopmental disorders are still not well understood, and effective treatments are lacking. Here, using stable-isotope labeling of amino acids in mammals and ubiquitination assays, we identify PTPA, an activator of protei...
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#1Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
#2Dinshaw J. Patel (SU: Southern University and A&M College)H-Index: 2
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#1Haoyi Wang (CAS: Chinese Academy of Sciences)H-Index: 25
#2Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
During the second World Summit of Human Gene Editing, Jiankui He presented the gene-editing project that led to the birth of two baby girls with man-made C-C chemokine receptor type 5 (CCR5) mutations. This extremely irresponsible behavior violated the ethical consensus of scientists all over the world. His presentation revealed a troubling lack not only of basic medical ethics but also of the requisite understanding of genetics and gene editing. Here, we review the rationale and design of his e...
3 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Yidi Sun (CAS-MPG Partner Institute for Computational Biology)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 10 authors...
Genome editing holds promise for correcting pathogenic mutations. However, it is difficult to determine off-target effects of editing due to single nucleotide polymorphism in individuals. Here, we developed a method named GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) to detect off-target mutations by editing one blastomere of two-cell mouse embryos using either CRISPR-Cas9 or base editors. Comparison of the whole genome sequences of progeny cells of edited vs. non-edited bl...
66 CitationsSource
#1Guang Yang (CAS: Chinese Academy of Sciences)H-Index: 9
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Yunbo Qiao (GU: Guangzhou University)H-Index: 1
view all 15 authors...
The coactivator-associated arginine methyltransferase CARM1 catalyzes the methylation of histone H3 arginine 17/26 (H3R17/26me) and non-histone proteins at arginine residues to regulate gene transactivation through profiling or Carm1 overexpression assays. However, the direct relationship between H3R17/26me and its causal role in mouse embryo development remains largely unclear. Here, we use rAPOBEC1-XTEN-Cas9n-UGI (BE3) to efficiently introduce a point mutation (R17H) at multiple Hist1/2H3 loci...
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#1Hui YangH-Index: 19
#2LIYixueH-Index: 57
Last. Lars M. Steinmetz (Stanford University)H-Index: 56
view all 8 authors...
Genome editing tools including CRISPR/Cas9 and base editors hold great promise for correcting pathogenic mutations. Unbiased genome-wide off-target effects of the editing in mammalian cells is required before clinical applications, but determination of the extent of off-target effects has been difficult due to the existence of single nucleotide polymorphisms (SNPs) in individuals. Here, we developed a method named GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) to detect off-...
4 CitationsSource
#1He Zhang (CAS: Chinese Academy of Sciences)H-Index: 2
#2Hong Pan (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Zhen Liu (CAS: Chinese Academy of Sciences)H-Index: 48
view all 17 authors...
ABSTRACT In vivo genetic mutation has become a powerful tool for dissecting gene function; however, multi-gene interaction and the compensatory mechanisms involved can make findings from single mutations, at best difficult to interpret, and, at worst, misleading. Hence, it is necessary to establish an efficient way to disrupt multiple genes simultaneously. CRISPR/Cas9-mediated base editing disrupts gene function by converting a protein-coding sequence into a stop codon; this is referred to as CR...
6 CitationsSource
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