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Xiangdong Gao
China Pharmaceutical University
112Publications
20H-index
1,403Citations
Publications 112
Newest
Interleukin (IL)-23 is considered an effective therapeutic target for the treatment of psoriasis because of the crucial role of the IL-23/IL-17 axis in the pathogenesis of psoriasis, and it has recently been reported to be involved in ILC3 cell differentiation. In this study, we report that eukaryotically expressed rhIL23R-CHR/Fc, as an endogenous extracellular receptor analogue, could be a natural antagonist in an imiquimod (IMQ)-induced psoriasis-like mouse model, including the antagonizing ef...
#1Xiaoda Song (CPU: China Pharmaceutical University)H-Index: 1
#2Xiaofei Chen (CPU: China Pharmaceutical University)H-Index: 2
Last.Wenbing Yao (CPU: China Pharmaceutical University)H-Index: 17
view all 7 authors...
Abstract Designed ankyrin repeat proteins (DARPins) are a kind of proteins containing the similar characteristics to antibodies. Which can specifically bind the target with high affinity. Here, we described an efficient method to select of DARPins effectively against the programmed death-ligand 1(PD-L1), a prominent target for cancer immune therapy, by E. coli surface display. E. coli surface display combined with magnetic cell sorting (MACS) were easy to manipulate with the advantages of solubi...
#1Huan Li (CPU: China Pharmaceutical University)H-Index: 1
#2Zichen Wang (CPU: China Pharmaceutical University)H-Index: 1
Last.Wenying Liu (CPU: China Pharmaceutical University)H-Index: 14
view all 6 authors...
Abstract Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide were extracted from S. glabra . The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined. The results exhibited that the two proteoglycans had pseudoplastic fluids properties and displayed shear-thinning behavior. The apparent viscosity of the two proteoglycans both increased with increasing ...
#1Kaidong Li (CPU: China Pharmaceutical University)
#2Lingling Zhu (CPU: China Pharmaceutical University)
Last.Wenying Liu (CPU: China Pharmaceutical University)H-Index: 14
view all 7 authors...
Abstract A new pectin (ALP-2) was extracted from the roots of Arctium lappa L. with the molecular weight of 1.84 × 10 6 Da. ALP-2 was composed of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose. Results of NMR revealed that the dominant linkage types of ALP-2 were →4-α-GalpA-6-OMe-(1→, →2-α-Rha-(1→, →5-α-Araf-(1→ and →3,6-β-Galp-(1→. The ELISA results indicated ALP-2 was a typical pectin with HG chain and RG-I chain. The rheological experiments showed that ...
#1Xiaoda Song (CPU: China Pharmaceutical University)H-Index: 1
#2Yuting Yi (CPU: China Pharmaceutical University)H-Index: 1
Last.Xiangdong Gao (CPU: China Pharmaceutical University)H-Index: 20
view all 8 authors...
Abstract Monellin, one kind of most sweet proteins, could be used instead of carbohydrates in the diabetic diet and pharmaceuticals. However, the critical problem interferes with monellin usage in the food or pharmaceutical industry was its unstable at high temperatures. Here, we describe a novel method to increase the thermostability of monellin. Inspired by the high T m value in RNA hairpin containing structure, the beta-hairpin was added between the two polypeptide chains of monellin instead ...
#1Jun Yin (CPU: China Pharmaceutical University)H-Index: 3
#2Dingkang Liu (CPU: China Pharmaceutical University)
Last.Xiangdong Gao (CPU: China Pharmaceutical University)H-Index: 20
view all 9 authors...
Abstract The great therapeutic potential of peptides has not yet been achieved, mainly due to their remarkably short in vivo half-life. Although conjugation to macromolecules has been an effective way of improving protein in vivo half-life, the steric hindrance of macromolecules usually reduces the in vivo efficacy of peptides. Here we report a complex delivery system made from PsTag polypeptide, polyglutamic acid chain, matrix metalloproteinase 2 (MMP2)-degradable domain and cationic cell penet...
#1Qingqing Wei (CPU: China Pharmaceutical University)
#2Hong Tian (CPU: China Pharmaceutical University)H-Index: 8
Last.Wenbing Yao (CPU: China Pharmaceutical University)H-Index: 17
view all 7 authors...
Abstract Given that the biological functions of proteins may decrease or even be lost due to degradation by proteases, it is of great significance to identify potential proteases that degrade protein drugs during systemic circulation. In this work, we describe a method based on high-performance liquid chromatography (HPLC) to identify key proteases that degrade therapeutic proteins in blood, including endopeptidases and exopeptidases. Here, the degradation of proteins was detected by competition...
#1Song Chen (CPU: China Pharmaceutical University)H-Index: 10
#2Suting Chen (CPU: China Pharmaceutical University)H-Index: 1
Last.Xiangdong Gao (CPU: China Pharmaceutical University)H-Index: 20
view all 8 authors...
Abstract Our understanding of the mechanisms underlying process in Alzheimer's disease (AD) is far from completion and new therapeutic targets are urgently needed. Recently, the link between dementia and diabetes mellitus (DM) prompted us to search for new therapeutic strategies from glucose metabolism regulators for neurodegeneration. Previous studies have indicated that fibroblast growth factor 21 (FGF21), an attractive and potential therapeutic treatment for DM, may exert diverse effects in t...
#1Jun Yin (CPU: China Pharmaceutical University)H-Index: 3
#2Shan Hou (CPU: China Pharmaceutical University)H-Index: 1
Last.Xiangdong Gao (CPU: China Pharmaceutical University)H-Index: 20
view all 8 authors...
Successful and efficient delivery of Cas9 protein and gRNA into cells is critical for genome editing and its therapeutic application. In this study, we developed an improved supercharged polypeptide (SCP) mediated delivery system based on dithiocyclopeptide linker to realize the effective genome editing in tumor cells. The fusion protein Cas9-linker-SCP (Cas9-LS) forms positively charged complexes with gRNA in vitro to provide possibilities for gRNA delivery into cells. Under the microenvironmen...
#1Yue Tong (CPU: China Pharmaceutical University)H-Index: 5
#2Xu Fang (CPU: China Pharmaceutical University)H-Index: 1
Last.Wenbing Yao (CPU: China Pharmaceutical University)H-Index: 17
view all 7 authors...
ABSTRACTIn vitro immunization can to used to produce monoclonal antibodies(mAbs), but this technology is limited by poor reproducibility and the requirement of pre-immunized lymphocytes. To improve this approach, we recently developed a method for rapid generation of antigen-specific B cells. Here, we report the application of this system to the production of human IgGs against tumor necrosis factor (TNF). We expressed mutant proteins with site-specific incorporated p-nitrophenylalanine (pNO2Phe...
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