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Deepika Awasthi
Central Drug Research Institute
ImmunologyNitric oxide synthaseNADPH oxidaseBiochemistryBiology
13Publications
7H-index
129Citations
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Publications 14
Newest
#1Samreen Sadaf (Central Drug Research Institute)H-Index: 1
#2Deepika Awasthi (Central Drug Research Institute)H-Index: 7
Last. Madhu Dikshit (Central Drug Research Institute)H-Index: 34
view all 7 authors...
Abstract Previous studies from this lab and others have demonstrated that nitric oxide (NO) in a concentration dependent manner, modulated neutrophil and leukemic cell survival. Subsequent studies delineated importance of iNOS in neutrophil differentiation and leukemic cell death. On the contrary, role of nNOS in survival of these cells remains least understood. Present study was therefore undertaken to assess and compare the role of iNOS and nNOS in the survival of NOS overexpressing myelocytic...
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#1Deepika Awasthi (Central Drug Research Institute)H-Index: 7
#2Sheela Nagarkoti (Central Drug Research Institute)H-Index: 5
Last. Madhu Dikshit (Central Drug Research Institute)H-Index: 34
view all 6 authors...
Abstract Neutrophil extracellular traps (NETs) play a pivotal role in the innate immune defense, as well as in the pathophysiology of various inflammatory disease conditions. Two major types of NETosis have been described - NOX-dependent and independent. The present study was undertaken to assess metabolic requirements of NETs formation by using PMA and A23187 as the inducers of NOX-dependent and NOX-independent NETosis respectively. Both these inducers caused an increase in ECAR, lactate dehydr...
1 CitationsSource
#1Sheela Nagarkoti (Central Drug Research Institute)H-Index: 5
#2Megha Dubey (Central Drug Research Institute)H-Index: 8
Last. Madhu Dikshit (Translational Health Science and Technology Institute)H-Index: 1
view all 8 authors...
Neutrophil survival and oxidative stress during inflammatory conditions are linked to tissue damage. The present study explores less understood role of catalase, the enzyme catalysing hydrogen peroxide decomposition, in neutrophil survival/death. Importantly, inhibition of catalase activity following S-glutathionylation in the PMA, NO, or zymosan-activated neutrophils or treatment with catalase inhibitor led to neutrophil death. On the contrary, introducing reducing environment by TCEP rescued c...
Source
#1Samreen Sadaf (Central Drug Research Institute)H-Index: 1
#2Abhishek Singh (Central Drug Research Institute)H-Index: 11
Last. Madhu Dikshit (Central Drug Research Institute)H-Index: 34
view all 10 authors...
1 CitationsSource
#1Sheela Nagarkoti (Central Drug Research Institute)H-Index: 5
#2Samreen Sadaf (Central Drug Research Institute)H-Index: 1
Last. Madhu DikshitH-Index: 34
view all 7 authors...
Recent report from this lab has shown role of Rac2 in the translocation of inducible nitric oxide synthase (iNOS) to the phagosomal compartment of polymorphonuclear leukocytes (PMNs) following phagocytosis of beads. This study was undertaken to further assess the status and role of tetrahydrobiopterin (BH4), a redox-sensitive cofactor, L-arginine, and the substrate of nitric oxide synthase (NOS) in sustained nitric oxide (˙NO) production in killing of phagocytosed microbes (Escherichia coli) by ...
1 CitationsSource
#1Babu Nageswararao Kanuri (CSIR: Council of Scientific and Industrial Research)
#2Sanjay C. Rebello (CSIR: Council of Scientific and Industrial Research)
Last. Madhu Dikshit (Indian Institute of Technology, Jodhpur)
view all 10 authors...
AbstractOxidative stress due to enhanced production or reduced scavenging of reactive oxygen species (ROS) has been associated with diet (dyslipidemia) induced obesity and insulin resistance (IR). The present study was undertaken to assess the role of p47phox in IR using wild type (WT) and p47phox−/− mice, fed with different diets (HFD, LFD or Chow). Augmented body weight, glucose intolerance and reduced insulin sensitivity were observed in p47phox−/− mice fed with 45% HFD and 10% LFD. Further, ...
Source
#1Sheela Nagarkoti (Central Drug Research Institute)H-Index: 5
#2Megha Dubey (Central Drug Research Institute)H-Index: 8
Last. Madhu Dikshit (Central Drug Research Institute)H-Index: 34
view all 7 authors...
Abstract Post-translational modifications (PTMs) induced conformational changes of proteins can cause their activation or inactivation. Neutrophils clear pathogen through phagocytosis and oxidative burst generation, while participate in inflammation through sustained and uncontrolled generation of ROS. In activated PMNs, cytosolic NOX-2 subunit p47phox following phosphorylation interacts with p67phox, p40phox and along with Rac2 translocate to the membrane. Phosphorylation of p47phox subunit occ...
10 CitationsSource
#1Piu Saha (PSU: Pennsylvania State University)H-Index: 7
#2Beng San Yeoh (PSU: Pennsylvania State University)H-Index: 10
Last. Matam Vijay-Kumar (PSU: Pennsylvania State University)H-Index: 33
view all 12 authors...
Neutrophils are the primary immune cells that respond to inflammation and combat microbial transgression. To thrive, the bacteria residing in their mammalian host have to withstand the antibactericidal responses of neutrophils. We report that enterobactin (Ent), a catecholate siderophore expressed by Escherichia coli , inhibited PMA-induced generation of reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) in mouse and human neutrophils. Ent also impaired the degranulation of ...
16 CitationsSource
#1Megha Dubey (Central Drug Research Institute)H-Index: 8
#2Sheela Nagarkoti (Central Drug Research Institute)H-Index: 5
Last. Madhu Dikshit (Central Drug Research Institute)H-Index: 34
view all 8 authors...
Neutrophils play an indispensable role in killing of invading pathogens by enhancing reactive oxygen species (ROS) and NO generation, and subsequently undergoing apoptosis. Unlike ROS/NOX2, role of NO/NOS still remains undefined in the apoptosis of neutrophils (PMNs) and the present study attempts to decipher the importance of NO/NOS in the neutrophil apoptosis. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with NO led to enhanced ROS generation, caspase-8/casp...
22 CitationsSource
#1Abhishek Singh (Central Drug Research Institute)H-Index: 11
#2Deepika Awasthi (Central Drug Research Institute)H-Index: 7
Last. Madhu Dikshit (Central Drug Research Institute)H-Index: 34
view all 9 authors...
Increasing evidence support bimodal action of nitric oxide (NO) both as a promoter and as an impeder of oxygen free radicals in neutrophils (PMNs), however impact of high oxidative stress on NO generation is less explored. In the present study, we comprehensively investigated the effect of high oxidative stress on inducible nitric oxide synthase (iNOS) expression and NO generation in human PMNs. Our findings suggest that PMA or diamide induced oxidative stress in PMNs from healthy volunteers, an...
7 CitationsSource
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