Match!
Isao Morishita
Hoshi University
ChemistryProteaseInsulinAprotininDosage form
8Publications
5H-index
405Citations
What is this?
Publications 6
Newest
#1Tsuneji Nagai (Hoshi University)H-Index: 53
#2Mariko MorishitaH-Index: 1
Last. Yuji MakinoH-Index: 1
view all 5 authors...
From the pharmaceutical point of view, the bioavailability, that is the amount and rate of absorption, is the most important factor determining the drug quality with respect to its efficacy and safety. Then, the bioavailability is controlled by well-designed dosage forms, for which interactions with surrounding components are applicable. Practically there are afforded various possibilities of modification of the interaction and then of control of drug delivery by a design of dosage forms, using ...
Source
#1Isao Morishita (Hoshi University)H-Index: 5
#2Mariko Morishita (Hoshi University)H-Index: 38
Last. Tsuneji Nagai (Hoshi University)H-Index: 53
view all 5 authors...
Abstract Three types of insulin microspheres (IMS) were prepared in order to deliver insulin selectively in the intestinal tract using copolymers having pH-dependent solubility, Eudragit L100 (L), Eudragit S100 (S) and a 1:1 mixture of these (LS). In a release study, the insulin release rate from S-IMS was much slower than that from L-IMS at a pH below 7.0. At a pH of 7.5, all the IMS released more than 90% of the insulin within 60 min. Thus, the three types of IMS were expected to release insul...
64 CitationsSource
#1Mariko Morishita (Hoshi University)H-Index: 38
#2Isao Morishita (Hoshi University)H-Index: 5
Last. Tsuneji Nagai (Hoshi University)H-Index: 53
view all 5 authors...
In order to determine an advantageous site for intestinal insulin absorption, the hypoglycemic effects of insulin after administration to the duodenum, the jejunum, the ileum and the colon were investigated using an in situ loop method. Insulin solution was administered to the various loops of fasted rats with or without aprotinin (AP) as a protease inhibitor, or absorption enhancers such as sodium caprate, Na2EDTA or sodium glycocholate. An obvious hypoglycemic effect of insulin alone was seen ...
134 CitationsSource
#1Mariko Morishita (Hoshi University)H-Index: 38
#2Isao Morishita (Hoshi University)H-Index: 5
Last. Tsuneji Nagai (Hoshi University)H-Index: 53
view all 5 authors...
Abstract Eudragit L100 insulin microspheres (IMS) containing a protease inhibitor were prepared for an oral dosage form of insulin. The efficiencies of insulin incorporation of IMS containing trypsin inhibitor (TI), chymostatin (CS), Bowman-Birk inhibitor (BBI) and aprotinin (AP) amounted to approx. 80% and did not differ among the preparations. In a release study, the more than 90% of insulin remaining after treatment of simulated gastric fluid was released in a medium of simulated intestinal f...
67 CitationsSource
#1Isao Morishita (Hoshi University)H-Index: 5
#2Mariko Morishita (Hoshi University)H-Index: 38
Last. Tsuneji Nagai (Hoshi University)H-Index: 53
view all 5 authors...
Abstract The effectiveness of an oral administration of insulin microspheres (IMS) containing a protease inhibitor was evaluated in normal and diabetic rats. The dosage form is based on the incorporation of insulin with protease inhibitor into polyacrylic polymer (Eudragit L100). These preparations were administered orally with a 20 U/kg insulin dose by force-feeding to rats. Insulin absorption was evaluated by its hypoglycemic effect. IMS without protease inhibitor and with trypsin inhibitor (T...
110 CitationsSource
#1Isao MorishitaH-Index: 5
#2Mariko MorishitaH-Index: 38
Last. Tsuneji NagaiH-Index: 53
view all 4 authors...
22 Citations
1