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Miki Watanabe
University of Tokyo
10Publications
4H-index
146Citations
Publications 10
Newest
#1Miki Watanabe (UTokyo: University of Tokyo)H-Index: 4
#2Ken-ichi Miyazono (UTokyo: University of Tokyo)H-Index: 13
Last.Ichizo Kobayashi (UTokyo: University of Tokyo)H-Index: 39
view all 6 authors...
#1Ken-ichi MiyazonoH-Index: 13
#2Miki WatanabeH-Index: 4
Last.Ichizo KobayashiH-Index: 39
view all 8 authors...
#1Ken-ichi Miyazono (UTokyo: University of Tokyo)H-Index: 13
#2Miki Watanabe (UTokyo: University of Tokyo)H-Index: 4
Last.Ichizo Kobayashi (UTokyo: University of Tokyo)H-Index: 39
view all 11 authors...
Although structures of many DNA-binding proteins have been solved, they fall into a limited number of folds. Here, we describe an approach that led to the finding of a novel DNA-binding fold. Based on the behavior of Type II restriction–modification gene complexes as mobile elements, our earlier work identified a restriction enzyme, R.PabI, and its cognate modification enzyme in Pyrococcus abyssi through comparison of closely related genomes. While the modification methyltransferase was easily r...
#1Ken Ishikawa (UTokyo: University of Tokyo)H-Index: 6
#2Miki Watanabe (UTokyo: University of Tokyo)H-Index: 4
Last.Ichizo Kobayashi (UTokyo: University of Tokyo)H-Index: 39
view all 8 authors...
#1Ichizo Kobayashi (UTokyo: University of Tokyo)H-Index: 39
#2Ken Ishikawa (UTokyo: University of Tokyo)H-Index: 6
Last.Miki Watanabe (UTokyo: University of Tokyo)H-Index: 4
view all 3 authors...
Genome sequence comparisons among multiple species of Pyrococcus, a hyperthermophilic archaeon, revealed a linkage between a putative restriction-modification gene complex and several large genome polymorphisms/rearrangements. From a region apparently inserted into the Pyrococcus abyssi genome, a hyperthermoresistant restriction enzyme [PabI; 5′-(GTA/C)] with a novel structure was discovered. In the present work, the neighboring methyltransferase homologue, M.PabI, was characterized. Its N-termi...
#1Ken IshikawaH-Index: 6
#2Miki WatanabeH-Index: 4
Last.Ichizo KobayashiH-Index: 39
view all 8 authors...
To search for restriction endonucleases, we used a novel plant-based cell-free translation procedure that bypasses the toxicity of these enzymes. To identify candidate genes, the related genomes of the hyperthermophilic archaea Pyrococcus abyssi and Pyrococcus horikoshii were compared. In line with the selfish mobile gene hypothesis for restriction– modification systems, apparent genome rearrangement around putative restriction genes served as a selecting criterion. Several candidate restriction...
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