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Keiho Chiyo
Tokyo Medical and Dental University
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#1Tomomi Aida (Tokyo Medical and Dental University)H-Index: 11
#2Keiho Chiyo (Tokyo Medical and Dental University)H-Index: 1
Last.Kohichi Tanaka (Tokyo Medical and Dental University)H-Index: 51
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Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target digestion, leading to generation of knock-in mice carrying a functional cassette with up to 50% efficiency, compared with just 10% by a commonly used method consisting of Cas9 mRNA and single guide RNA...
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