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Taehan Kim
Yale University
7Publications
5H-index
149Citations
Publications 7
Newest
#1Samir GautamH-Index: 7
#2Taehan KimH-Index: 5
Last.D. SpeigelH-Index: 89
view all 5 authors...
Staphylococcus aureus is a Gram-positive bacterial pathogen that produces a range of infections including cellulitis, pneumonia, and septicemia. The principle mechanism in antistaphylococcal host defense is opsonization with antibodies and complement proteins, followed by phagocytic clearance. Here we use a previously developed technique for installing chemical epitopes in the peptidoglycan cell wall to show that surface glycopolymers known as wall teichoic acids conceal cell wall epitopes, prev...
#1Samir Gautam (Yale University)H-Index: 7
#2Taehan Kim (Yale University)H-Index: 5
Last.D. Speigel (Yale University)H-Index: 89
view all 9 authors...
Penicillin-binding proteins (PBPs) catalyze the crosslinking of peptidoglycan (PG), an essential process for bacterial growth and survival, and a common antibiotic target. Yet, despite its importance, little is known about the spatiotemporal aspects of crosslinking—largely because of a lack of experimental tools for studying the reaction in live bacteria. Here we introduce such a tool: an activity-based probe that enables visualization and relative quantitation of crosslinking in vivo. In Staphy...
Staphylococcus aureus is an important human pathogen and a model organism for studying cell wall synthesis in Gram-positive cocci. The prevailing model of cell wall biogenesis in cocci holds that peptidoglycan synthesis (i.e., transglycosylation and cross-linking) is restricted spatially to the septal cross-wall and temporally to cell division. Previously, we developed a method for visualizing cross-linking in S. aureus using fluorescently tagged mimics of the endogenous substrate of penicillin-...
#1Rachel J. Perry (Yale University)H-Index: 15
#2Taehan Kim (Yale University)H-Index: 5
Last.Gary W. Cline (Yale University)H-Index: 68
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Summary Nonalcoholic fatty liver disease (NAFLD) affects one in three Americans and is a major predisposing condition for the metabolic syndrome and type 2 diabetes (T2D). We examined whether a functionally liver-targeted derivative of 2,4-dinitrophenol (DNP), DNP-methyl ether (DNPME), could safely decrease hypertriglyceridemia, NAFLD, and insulin resistance without systemic toxicities. Treatment with DNPME reversed hypertriglyceridemia, fatty liver, and whole-body insulin resistance in high-fat...
#1Taehan Kim (Yale University)H-Index: 5
#2SpiegelDavid (Yale University)H-Index: 89
Last.D. Speigel (Yale University)H-Index: 89
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Abstract Herein, we report the serendipitous discovery of two highly selective inhibitors of bacterial luciferase, N -phenacylthiazolium bromide (PTB) and pifithrin-α (PFT-α). These compounds, which are both derivatives of N -phenacylthiazolium salts, comprise a previously unreported class of luciferase inhibitors that are effective both in vitro and in live bacteria. Interestingly, although they are structurally similar, these compounds appear to elicit inhibitory effects via two different mode...
#1Samir Gautam (Yale University)H-Index: 7
#2Thomas J. Gniadek (Johns Hopkins University)H-Index: 5
Last.D. Speigel (Yale University)H-Index: 89
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Recombinant techniques for expressing heterologous proteins and sugars on the surface of bacteria have been known since the 1980s, and have proven useful in a variety of settings from biocatalysis to vaccinology. The past decade has also seen the emergence of novel methods that allow modification of bacterial surfaces with small non-biological compounds. Such technologies enable researchers to harness the unique properties of synthetic materials on a live bacterial platform, opening the door to ...
#1Taehan KimH-Index: 5
#2SpiegelDavidH-Index: 89
Last.D. SpeigelH-Index: 89
view all 2 authors...
Abstract Advanced glycation end products (AGEs), a heterogeneous mixture of compounds formed by non-enzymatic chemical reactions between sugars and the nucleophilic residues of proteins, have been implicated in the pathogenesis of a number of diseases. ALT-711 is an N-phenacyl-derived thiazolium carbene developed as a therapeutic agent for cardiovascular diseases that is proposed to function through cleaving preformed AGE–protein cross-links. However, despite promising results in animal models a...
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