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Alexandre Denadai-Souza
University of Toulouse
EndocrinologyReceptorArthritisDiabetes mellitusMedicine
27Publications
7H-index
220Citations
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Publications 27
Newest
#1Jean-Paul Motta (University of Toulouse)H-Index: 15
#2Alexandre Denadai-Souza (University of Toulouse)H-Index: 7
Last. Nathalie Vergnolle (University of Toulouse)H-Index: 62
view all 23 authors...
Proteolytic homeostasis is important at mucosal surfaces, but its actors and their precise role in physiology are poorly understood. Here we report that healthy human and mouse colon epithelia are a major source of active thrombin. We show that mucosal thrombin is directly regulated by the presence of commensal microbiota. Specific inhibition of luminal thrombin activity causes macroscopic and microscopic damage as well as transcriptomic alterations of genes involved in host-microbiota interacti...
Source
#1Julie Noguerol (University of Toulouse)
#2Pierre-Jean Roustan (University of Toulouse)
Last. Claire Racaud-Sultan (University of Toulouse)H-Index: 17
view all 13 authors...
Background Sexual dimorphism in biological responses is a critical knowledge for therapeutic proposals. However, gender differences in intestinal stem cell physiology have been poorly studied. Given the important role of the protease-activated receptor PAR2 in the control of colon epithelial primitive cells and cell cycle genes, we have performed a sex-based comparison of its expression and of the effects of PAR2 activation or knockout on cell proliferation and survival functions.
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#1Christel Devaud (University of Toulouse)H-Index: 12
#2Anne-Françoise Tilkin-Mariamé (University of Toulouse)H-Index: 4
Last. Audrey Ferrand (University of Toulouse)H-Index: 4
view all 14 authors...
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#1Tereza Bautzova (University of Toulouse)H-Index: 5
#2James R.F. Hockley (University of Cambridge)H-Index: 5
Last. Nicolas Cenac (University of Toulouse)H-Index: 29
view all 21 authors...
Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is characterized by chronic abdominal pain concurrent with altered bowel habit. Polyunsaturated fatty acid (PUFA) metabolites are increased in abundance in IBS and are implicated in the alteration of sensation to mechanical stimuli, which is defined as visceral hypersensitivity. We sought to quantify PUFA metabolites in patients with IBS and evaluate their role in pain. Quantification of PUFA metabolites by mass spectromet...
5 CitationsSource
#1Alexandre Denadai-Souza (University of Toulouse)H-Index: 7
#2Chrystelle Bonnart (University of Toulouse)H-Index: 5
Last. Céline Deraison (University of Toulouse)H-Index: 11
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While proteases are essential in gastrointestinal physiology, accumulating evidence indicates that dysregulated proteolysis plays a pivotal role in the pathophysiology of inflammatory bowel disease (IBD). Nonetheless, the identity of overactive proteases released by human colonic mucosa remains largely unknown. Studies of protease abundance have primarily investigated expression profiles, not taking into account their enzymatic activity. Herein we have used serine protease-targeted activity-base...
6 CitationsSource
#1Morgane Sébert (University of Toulouse)H-Index: 1
#2Alexandre Denadai-Souza (University of Toulouse)H-Index: 7
Last. Nathalie Vergnolle (University of Toulouse)H-Index: 62
view all 13 authors...
Experimental Approach: Crypts were isolated from human colonic resections and cultured for 6 days, forming human colon organoids. Cultured organoids were exposed to 10 and 50 mU·mL−1 of thrombin, in the presence or not of protease‐activated receptor (PAR) antagonists. Organoid morphology, metabolism, proliferation and apoptosis were followed. Key Results: Thrombin favoured organoid maturation leading to a decreased number of immature cystic structures and a concomitant increased number of larger...
4 CitationsSource
#2Dafne BalemansH-Index: 3
Last. Guy E. BoeckxstaensH-Index: 52
view all 11 authors...
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#2Dafne BalemansH-Index: 3
Last. Guy E. BoeckxstaensH-Index: 52
view all 15 authors...
Source
#1Alexandre Denadai-Souza (University of Toulouse)H-Index: 7
#2Camilla M. Ribeiro (UNIFESP: Federal University of São Paulo)H-Index: 4
Last. Maria Christina W. Avellar (UNIFESP: Federal University of São Paulo)H-Index: 21
view all 9 authors...
Background Increasing evidences indicate that an unbalance between tryptases and their endogenous inhibitors, leading to an increased proteolytic activity, is implicated in the pathophysiology of rheumatoid arthritis. The aim of the present study was to evaluate the impact of tryptase inhibition on experimental arthritis.
7 CitationsSource
#1Claire Rolland-Fourcade (University of Toulouse)H-Index: 1
#2Alexandre Denadai-Souza (University of Toulouse)H-Index: 7
Last. Nathalie Vergnolle (University of Toulouse)H-Index: 62
view all 20 authors...
Objectives Proteases are key mediators of pain and altered enteric neuronal signalling, although the types and sources of these important intestinal mediators are unknown. We hypothesised that intestinal epithelium is a major source of trypsin-like activity in patients with IBS and this activity signals to primary afferent and enteric nerves and induces visceral hypersensitivity. Design Trypsin-like activity was determined in tissues from patients with IBS and in supernatants of Caco-2 cells sti...
26 CitationsSource
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