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Shintaro Yamada
Memorial Sloan Kettering Cancer Center
3Publications
3H-index
122Citations
Publications 3
Newest
Published on Oct 18, 2017in Cell Cycle3.26
Shintaro Yamada3
Estimated H-index: 3
(MSK: Memorial Sloan Kettering Cancer Center),
Seoyoung Kim3
Estimated H-index: 3
(MSK: Memorial Sloan Kettering Cancer Center)
+ 3 AuthorsScott Keeney47
Estimated H-index: 47
(MSK: Memorial Sloan Kettering Cancer Center)
ABSTRACTThe SPO11-generated DNA double-strand breaks (DSBs) that initiate meiotic recombination occur non-randomly across genomes, but mechanisms shaping their distribution and repair remain incompletely understood. Here, we expand on recent studies of nucleotide-resolution DSB maps in mouse spermatocytes. We find that trimethylation of histone H3 lysine 36 around DSB hotspots is highly correlated, both spatially and quantitatively, with trimethylation of H3 lysine 4, consistent with coordinated...
Published on Oct 1, 2016in Cell36.22
Julian Lange12
Estimated H-index: 12
(MSK: Memorial Sloan Kettering Cancer Center),
Shintaro Yamada3
Estimated H-index: 3
(MSK: Memorial Sloan Kettering Cancer Center)
+ 6 AuthorsScott Keeney47
Estimated H-index: 47
(Cornell University)
Summary Heritability and genome stability are shaped by meiotic recombination, which is initiated via hundreds of DNA double-strand breaks (DSBs). The distribution of DSBs throughout the genome is not random, but mechanisms molding this landscape remain poorly understood. Here, we exploit genome-wide maps of mouse DSBs at unprecedented nucleotide resolution to uncover previously invisible spatial features of recombination. At fine scale, we reveal a stereotyped hotspot structure—DSBs occur withi...
Published on 2016in bioRxiv
Eleni P. Mimitou1
Estimated H-index: 1
(MSK: Memorial Sloan Kettering Cancer Center),
Shintaro Yamada3
Estimated H-index: 3
(MSK: Memorial Sloan Kettering Cancer Center),
Scott Keeney47
Estimated H-index: 47
(MSK: Memorial Sloan Kettering Cancer Center)
The DNA double-strand breaks that initiate homologous recombination during meiosis are subject to extensive 5′→3′ exonucleolytic processing. This resection is a central and conserved feature of recombination, yet its mechanism is poorly understood. Using a purpose-made deep-sequencing method, we mapped meiotic resection endpoints genome-wide at high spatial resolution in Saccharomyces cerevisiae. Generating full-length resection tracts requires Exo1 exonuclease activity and the DNA-damage respon...
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