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Yen-Cheng Chen
Georgia Institute of Technology
Dark stateChemistryFluorophoreIntracellularFluorescence
4Publications
3H-index
30Citations
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#1Yen-Cheng Chen (Emory University)H-Index: 3
#2Chetan Sood (Emory University)H-Index: 4
Last. Robert M. Dickson (Georgia Institute of Technology)H-Index: 38
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Live cell fluorescence imaging is the method of choice for studying dynamic processes, such as nuclear transport, vesicular trafficking, and virus entry and egress. However, endogenous cellular autofluorescence masks a useful fluorescence signal, limiting the ability to reliably visualize low-abundance fluorescent proteins. Here, we employed synchronously amplified fluorescence image recovery (SAFIRe), which optically alters ground versus photophysical dark state populations within fluorescent p...
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Photoswitchable fluorescent proteins (PS-FPs) open grand new opportunities in biological imaging. Through optical manipulation of FP emission, we demonstrate that dual-laser modulated synchronously amplified fluorescence image recovery (DM-SAFIRe) improves signal contrast in high background through unambiguous demodulation and is linear in relative fluorophore abundance at different points in the cell. The unique bright-to-dark state interconversion rates of each PS-FP not only enables discrimin...
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