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Maryna Levikova
University of Zurich
13Publications
7H-index
322Citations
Publications 13
Newest
#1Lepakshi Ranjha (USI: University of Lugano)H-Index: 7
#2Maryna Levikova (UZH: University of Zurich)H-Index: 7
Last.Petr Cejka (ETH Zurich)H-Index: 28
view all 5 authors...
Dna2 is an essential nuclease-helicase that acts in several distinct DNA metabolic pathways including DNA replication and recombination. To balance these functions and prevent unscheduled DNA degradation, Dna2 activities must be regulated. Here we show that Saccharomyces cerevisiae Dna2 function is controlled by sumoylation. We map the sumoylation sites to the N-terminal regulatory domain of Dna2 and show that in vitro sumoylation of recombinant Dna2 impairs its nuclease but not helicase activit...
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#1Kristina Kasaciunaite (Leipzig University)H-Index: 2
#2Fergus Fettes (Leipzig University)H-Index: 1
Last.Ralf Seidel (WWU: University of Münster)H-Index: 37
view all 7 authors...
1 CitationsSource
#2Fergus FettesH-Index: 1
Last.Ralf SeidelH-Index: 37
view all 6 authors...
DNA double-strand break repair by homologous recombination employs long-range resection of the 59 DNA ends at the break points. In Saccharomyces cerevisiae, this process can be performed by the RecQ helicase Sgs1 and the helicase-nuclease Dna2. Though functional interplay has been shown, it remains unclear whether and how the proteins cooperate on the molecular level. Here, we resolved the dynamics of DNA unwinding by Sgs1 at the single molecule level and investigated its regulation by Dna2, the...
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#2Fergus FettesH-Index: 1
Last.Ralf SeidelH-Index: 37
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#1Magdalena K. Poplutz (RWTH Aachen University)H-Index: 2
#2Maryna Levikova (UZH: University of Zurich)H-Index: 7
Last.Michael Huber (RWTH Aachen University)H-Index: 14
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Stimulation with lipopolysaccharide (LPS; endotoxin) not only causes rapid production of proinflammatory cytokines, but also induces a state of LPS hypo-responsiveness to a second LPS stimulation (endotoxin tolerance (ET)). Murine bone marrow-derived MCs (BMMCs) and peritoneal MCs (PMCs) developed ET as shown by an abrogated production of Il6/Tnf RNAs and IL-6/TNF-α proteins. In naive BMMCs, LPS stimulation induced a transient decline in the trimethylation of lysine 9 of the core histone H3 (H3K...
3 CitationsSource
#1Maryna Levikova (UZH: University of Zurich)H-Index: 7
#2Cosimo Pinto (UZH: University of Zurich)H-Index: 5
Last.Petr Cejka (USI: University of Lugano)H-Index: 28
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16 CitationsSource
#1Gizem Ölmezer (University of Basel)H-Index: 1
#2Maryna Levikova (UZH: University of Zurich)H-Index: 7
Last.Ulrich Rass (FMI: Friedrich Miescher Institute for Biomedical Research)H-Index: 12
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Cells have evolved mechanisms to protect, restart and repair perturbed replication forks, allowing full genome duplication, even under replication stress. Interrogating the interplay between nuclease-helicase Dna2 and Holliday junction (HJ) resolvase Yen1, we find the Dna2 helicase activity acts parallel to homologous recombination (HR) in promoting DNA replication and chromosome detachment at mitosis after replication fork stalling. Yen1, but not the HJ resolvases Slx1-Slx4 and Mus81-Mms4, safe...
14 CitationsSource
#1Felix E. Kemmerich (WWU: University of Münster)H-Index: 4
#2Peter Daldrop (WWU: University of Münster)H-Index: 3
Last.Ralf Seidel (WWU: University of Münster)H-Index: 37
view all 6 authors...
Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release...
12 CitationsSource
#1Maryna Levikova (UZH: University of Zurich)H-Index: 7
#2Petr Cejka (UZH: University of Zurich)H-Index: 28
During DNA replication, synthesis of the lagging strand occurs in stretches termed Okazaki fragments. Before adjacent fragments are ligated, any flaps resulting from the displacement of the 5′ DNA end of the Okazaki fragment must be cleaved. Previously, Dna2 was implicated to function upstream of flap endonuclease 1 (Fen1 or Rad27) in the processing of long flaps bound by the replication protein A (RPA). Here we show that Dna2 efficiently cleaves long DNA flaps exactly at or directly adjacent to...
24 CitationsSource
#1Saravanabhavan Thangavel (SLU: Saint Louis University)H-Index: 4
#2Matteo Berti (SLU: Saint Louis University)H-Index: 9
Last.Alessandro Vindigni (SLU: Saint Louis University)H-Index: 19
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Accurate processing of stalled or damaged DNA replication forks is paramount to genomic integrity and recent work points to replication fork reversal and restart as a central mechanism to ensuring high-fidelity DNA replication. Here, we identify a novel DNA2- and WRN-dependent mechanism of reversed replication fork processing and restart after prolonged genotoxic stress. The human DNA2 nuclease and WRN ATPase activities functionally interact to degrade reversed replication forks with a 5′-to-3′ ...
134 CitationsSource
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