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J.M. Minina
Russian Academy of Sciences
12Publications
6H-index
114Citations
Publications 12
Newest
#1Inna E. Pristyazhnyuk (RAS: Russian Academy of Sciences)H-Index: 5
#2J.M. Minina (RAS: Russian Academy of Sciences)H-Index: 6
Last.O. L. Serov (NSU: Novosibirsk State University)H-Index: 11
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In a previous study using one-step CRISPR/Cas9 genome editing in mouse zygotes, we created five founders carrying a 1,137 kb deletion and two founders carrying the same deletion, plus a 2,274 kb duplication involving the Cntn6 gene (encoding contactin-6). Using these mice, the present study had the following aims: (i) to establish stage of origin of these rearrangements; (ii) to determine the fate of the deleted DNA fragments; and (iii) to estimate the scale of unpredicted DNA changes accompanyi...
Last.S. P. MedvedevH-Index: 8
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Abstract The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differenti...
Last.Suren M. ZakianH-Index: 18
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Abstract Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by mutation in the HTT gene encoding HTT protein. The mutant protein leads to the neuronal death through dysregulation of multiple cellular processes. HD human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study. iPSC line from HD patient with 47 CAG repeats in HTT was generated from blood mononuclear cells by non-integrating episomal vectors. The iPSC line ret...
#1Elena V. Grigor'eva (RAS: Russian Academy of Sciences)H-Index: 4
#2Tuyana B. Malankhanova (RAS: Russian Academy of Sciences)H-Index: 2
Last.Suren M. Zakian (RAS: Russian Academy of Sciences)H-Index: 18
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Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is a powerful method that is necessary for a broad range of studies including substitutive cell therapy and transplantology. Patient specific iPSCs differentiated into relevant cell types represent a valuable model to study pathological mechanisms of inherited diseases at the molecular level. iPSCs from healthy donors are promising for generation of isogenic cell lines harboring mutations in particular genes of interest u...
Previously, we described the unique feature of telomeric regions in Iberian shrew Sorex granarius: its telomeres have two ranges of size, very small (3.8 kb of telomeric repeats on average) and very large discontinuous telomeres (213 kb) interrupted with 18S rDNA. In this study, we have demonstrated extraordinary replication pattern of S. granarius large telomeres that have not been shown before in other studied mammal. Using the ReD-FISH procedure, we observed prolonged, through S period, large...
#1N. S. Zhdanova (RAS: Russian Academy of Sciences)H-Index: 9
#2Irena Draskovic (Curie Institute)H-Index: 8
Last.Dmitry A. SkvortsovH-Index: 8
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The telomere structure in the Iberian shrew Sorex granarius is characterized by unique, striking features, with short arms of acrocentric chromosomes carrying extremely long telomeres (up to 300 kb) with interspersed ribosomal DNA (rDNA) repeat blocks. In this work, we investigated the telomere physiology of S. granarius fibroblast cells and found that telomere repeats are transcribed on both strands and that there is no telomere-dependent senescence mechanism. Although telomerase activity is de...
#1Mikhail Liskovykh (RAS: Russian Academy of Sciences)H-Index: 6
#2Ilya Chuykin (MDC: Max Delbrück Center for Molecular Medicine)H-Index: 3
Last.John J. Mullins (Edin.: University of Edinburgh)H-Index: 55
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The rat represents an important animal model that, in many respects, is superior to the mouse for dissecting behavioral, cardiovascular and other physiological pathologies relevant to humans. Derivation of induced pluripotent stem cells from rats (riPS) opens the opportunity for gene targeting in specific rat strains, as well as for the development of new protocols for the treatment of different degenerative diseases. Here, we report an improved lentivirus-based hit-and-run riPS derivation proto...
#1N. S. Zhdanova (RAS: Russian Academy of Sciences)H-Index: 9
#2J.M. Minina (RAS: Russian Academy of Sciences)H-Index: 6
Last.J. A. Londono-VallejoH-Index: 1
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It is shown that the size, localization, and structure of telomeres in the Iberian shrew (Sorex granarius) are not characteristic of mammals. In this species, long telomeres of an average size of 213 kb are localized on the short arms of all 32 acrocentrics; ribosomal blocks and active nucleolus-organizing regions (NORs) were also discovered there. At the remaining chromosome ends the average size of telomeres is 3.8 kb. However, in a closely related species, Sorex araneus, all telomeres have si...
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