Robert Fleming
University of Edinburgh
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Publications 46
#1Katherine Staines (Edinburgh Napier University)H-Index: 11
#1Katherine A. Staines (Edinburgh Napier University)H-Index: 1
Last. Colin Farquharson (Edin.: University of Edinburgh)H-Index: 33
view all 9 authors...
E11/Podoplanin (Pdpn) is implicated in early osteocytogenesis and the formation of osteocyte dendrites. This dendritic network is critical for bone modelling/remodelling, through the production of receptor activator of nuclear factor kappa B (RANK)-ligand (RANKL). Despite this, the role of Pdpn in the control of bone remodelling is yet to be established in vivo. Here we utilised bone-specific Pdpn conditional knockout mice (cKO) to examine the role of Pdpn in the bone loss associated with ovarie...
#1Dirk-Jan de Koning (SLU: Swedish University of Agricultural Sciences)H-Index: 14
Last. Alejandro B. Rodríguez-Navarro (UGR: University of Granada)H-Index: 28
view all 17 authors...
BACKGROUND: Skeletal damage is a challenge for laying hens because the physiological adaptations required for egg laying make them susceptible to osteoporosis. Previously, we showed that genetic factors explain 40% of the variation in end of lay bone quality and we detected a quantitative trait locus (QTL) of large effect on chicken chromosome 1. The aim of this study was to combine data from the commercial founder White Leghorn population and the F2 mapping population to fine-map this QTL and u...
#1Ekele Ikpegbu (Edin.: University of Edinburgh)H-Index: 4
#2Lena Basta (Edin.: University of Edinburgh)H-Index: 1
Last. Colin Farquharson (Edin.: University of Edinburgh)H-Index: 27
view all 9 authors...
E11/podoplanin is critical in the early stages of osteoblast-to-osteocyte transitions (osteocytogenesis), however, the upstream events which regulate E11 expression are unknown. The aim of this study was to examine the effects of FGF-2 on E11-mediated osteocytogenesis and to reveal the nature of the underlying signaling pathways regulating this process. Exposure of MC3T3 osteoblast-like cells and murine primary osteoblasts to FGF-2 (10 ng/ml) increased E11 mRNA and protein expression (p < 0.05) ...
4 CitationsSource
#1Biaty Raymond (SLU: Swedish University of Agricultural Sciences)
#2Anna M. Johansson (SLU: Swedish University of Agricultural Sciences)H-Index: 17
Last. Dirk-Jan de Koning (SLU: Swedish University of Agricultural Sciences)H-Index: 14
view all 7 authors...
Bone fracture in egg laying hens is a growing welfare and economic concern in the industry. Although environmental conditions and management (especially nutrition) can exacerbate it, the primary cause of bone weakness and the resulting fractures is believed to have a genetic basis. To test this hypothesis, we performed a genome-wide association study to identify the loci associated with bone strength in laying hens. Genotype and phenotype data were obtained from 752 laying hens belonging to the ...
#1D.J. de KoningH-Index: 27
Last. H. WallH-Index: 11
view all 7 authors...
#1Alejandro B. Rodríguez-Navarro (UGR: University of Granada)H-Index: 28
#2H.M. McCormack (Edin.: University of Edinburgh)H-Index: 1
Last. Ian F. Dunn (Edin.: University of Edinburgh)H-Index: 47
view all 8 authors...
Abstract Laying hens develop a type of osteoporosis that arises from a loss of structural bone, resulting in high incidence of fractures. In this study, a comparison of bone material properties was made for lines of hens created by divergent selection to have high and low bone strength and housed in either individual cages, with restricted mobility, or in an aviary system, with opportunity for increased mobility. Improvement of bone biomechanics in the high line hens and in aviary housing was ma...
5 CitationsSource
#1Katherine Staines (Edin.: University of Edinburgh)H-Index: 11
#2Behzad Javaheri (RVC: Royal Veterinary College)H-Index: 9
Last. Colin Farquharson (Edin.: University of Edinburgh)H-Index: 27
view all 10 authors...
The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by global Pdpn deletion, we have herein explored the effect of bone specific Pdpn knockdown on osteocyte form and function in the post-natal mouse. Extensive skeletal phenotyping of male and female 6-week-old Oc-cre;Pdpnflox/flox (cKO) mice and their Pdpn...
8 CitationsSource
#1Timothy Connelley (Edin.: University of Edinburgh)H-Index: 12
#2Kathryn Degnan (Edin.: University of Edinburgh)H-Index: 6
Last. Ivan Morrison (Edin.: University of Edinburgh)H-Index: 12
view all 8 authors...
#1Scott M. Nelson (Glas.: University of Glasgow)H-Index: 51
#2Martina Messow (Robertson Centre for Biostatistics)H-Index: 4
Last. B. LeaderH-Index: 2
view all 8 authors...
The value of anti-mullerian hormone (AMH) as a marker of the ovarian reserve is becoming clear in a range of clinical contexts. This study reports the external validation of a quadratic model-based AMH–age nomogram using a cohort of 15,834 US women. All models previously investigated for the decline in ovarian reserve (i.e. linear, bi-linear, decay curve, power and quadratic models) tended to overestimate AMH by approximately 11% versus the published nomogram, indicating some between-population ...
55 CitationsSource
#1A.M. Wallace (Glasgow Royal Infirmary)H-Index: 1
#2S.A. Faye (Beckman Coulter)H-Index: 1
Last. Scott M. Nelson (Glas.: University of Glasgow)H-Index: 51
view all 4 authors...
BackgroundThe measurement of anti-Mullerian hormone (AMH) has been by two commercial enzyme-linked immunosorbent (ELISA) assays: Diagnostics Systems Laboratory (DSL 10-14400) and Immunotech (A11893 IVD EU only). Beckman Coulter has developed a new assay for AMH (AMH Gen II A79765), which uses the DSL antibodies but is standardized to the Immunotech calibration. As a result, comparative data are urgently required between the old DSL assay and its replacement AMH Gen II.MethodsAn evaluation of the...
91 CitationsSource