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O. L. Serov
Novosibirsk State University
77Publications
11H-index
528Citations
Publications 77
Newest
#1Inna E. Pristyazhnyuk (RAS: Russian Academy of Sciences)H-Index: 5
#2J.M. Minina (RAS: Russian Academy of Sciences)H-Index: 6
Last.O. L. Serov (NSU: Novosibirsk State University)H-Index: 11
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In a previous study using one-step CRISPR/Cas9 genome editing in mouse zygotes, we created five founders carrying a 1,137 kb deletion and two founders carrying the same deletion, plus a 2,274 kb duplication involving the Cntn6 gene (encoding contactin-6). Using these mice, the present study had the following aims: (i) to establish stage of origin of these rearrangements; (ii) to determine the fate of the deleted DNA fragments; and (iii) to estimate the scale of unpredicted DNA changes accompanyi...
#1Aleksei G. Menzorov (Russian Academy)
#2Konstantin E. Orishchenko (Russian Academy)
Last.Michael Bader (LMB: Laboratory of Molecular Biology)H-Index: 108
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Neuronal tracing is a modern technology that is based on the expression of fluorescent proteins under the control of cell type-specific promoters. However, random genomic integration of the reporter construct often leads to incorrect spatial and temporal expression of the marker protein. Targeted integration (or knock-in) of the reporter coding sequence is supposed to provide better expression control by exploiting endogenous regulatory elements. Here we describe the generation of two fluorescen...
#1Alexander V. Smirnov (RAS: Russian Academy of Sciences)H-Index: 15
#2G. V. Kontsevaya (RAS: Russian Academy of Sciences)H-Index: 3
Last.O. L. Serov (NSU: Novosibirsk State University)H-Index: 11
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Contactins (Cntn1-6) are a family of neuronal membrane proteins expressed in the brain. They are required for establishing cell-to-cell contacts between neurons and for the growth and maturation of the axons. In humans, structural genomic variations in the Contactin genes are implicated in neurodevelopmental disorders. In addition, population genetic studies associate Contactins loci with obesity and hypertension. Cntn5 knockout mice were first described in 2003, but showed no gross physiologica...
#1Alexei N. KorablevH-Index: 1
#2Serova IaH-Index: 6
Last.O. L. SerovH-Index: 11
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Background Copy Number Variation (CNV) of the human CNTN6 gene (encoding the contactin-6 protein), caused by deletions or duplications, is responsible for severe neurodevelopmental impairments, often in combination with facial dysmorphias. Conversely, deleterious point mutations of this gene do not show any clinical phenotypes. The aim of this study is to generate mice carrying large deletions, duplications and inversions involving the Cntn6 gene as a new experimental model to study CNV of the h...
#1Aleksei G. Menzorov (NSU: Novosibirsk State University)H-Index: 4
#2N. M. Matveeva (RAS: Russian Academy of Sciences)H-Index: 8
Last.Razvan Anistoroaei (UCPH: University of Copenhagen)H-Index: 9
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Background Recently fibroblasts of many mammalian species have been reprogrammed to pluripotent state using overexpression of several transcription factors. This technology allows production of induced pluripotent stem (iPS) cells with properties similar to embryonic stem (ES) cells. The completeness of reprogramming process is well studied in such species as mouse and human but there is not enough data on other species. We produced American mink (Neovison vison) ES and iPS cells and compared th...
#1Nariman R. Battulin (Skolkovo Institute of Science and Technology)H-Index: 6
#2Veniamin S. Fishman (Skolkovo Institute of Science and Technology)H-Index: 3
Last.O. L. SerovH-Index: 11
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Background The three-dimensional organization of the genome is tightly connected to its biological function. The Hi-C approach was recently introduced as a method that can be used to identify higher-order chromatin interactions genome-wide. The aim of this study was to determine genome-wide chromatin interaction frequencies using the Hi-C approach in mouse sperm cells and embryonic fibroblasts.
#1Inna E. Pristyazhnyuk (RAS: Russian Academy of Sciences)H-Index: 5
#2T. A. Shnayder (RAS: Russian Academy of Sciences)
Last.O. L. Serov (RAS: Russian Academy of Sciences)H-Index: 11
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The generation of multipotent patient-specific neural precursors from human fibroblasts is one of the challenges to regenerative medicine. A recently proposed novel approach allows direct conversion of human and mouse fibroblasts into induced neuronal precursor (iNP) cells by overexpression of a single transcription factor, Sox2. In this work, we have analyzed the Sox2-induced iNP cells and evaluated the possibility of its medical application. Both mouse and human fibroblasts had pronounced morp...
#1R. I. T. P. Batista (State University of Ceará)H-Index: 5
#2Dárcio Ítalo Alves Teixeira (State University of Ceará)H-Index: 6
Last.V. J. F. Freitas (State University of Ceará)H-Index: 11
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The discovery of DNA has opened many areas of research including biotechnology and genetic engineering. The possibilities of isolation and introduction of genes into cells has become a routine procedure in laboratories around the world. Recombinant proteins of high economic value have been expressed in genetically-modified mammalian cells, which are better prepared for the synthesis of complex proteins. However, the need of large capital investment, high operating costs and relatively low produc...
#2N. M. MatveevaH-Index: 8
Last.Razvan AnistoroaeiH-Index: 9
view all 11 authors...
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