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Kristina Kasaciunaite
Leipzig University
Homologous recombinationHelicaseGeneticsDNABiology
6Publications
2H-index
33Citations
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Publications 7
Newest
#1Ilaria Ceppi (USI: University of Lugano)
#2Sean Michael Howard (USI: University of Lugano)H-Index: 3
Last. Petr Cejka (USI: University of Lugano)H-Index: 28
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BLM or WRN helicases function with the DNA2 helicase-nuclease to resect DNA double-strand breaks and initiate homologous recombination. Upon DNA unwinding by BLM/WRN, RPA directs the DNA2 nuclease to degrade the 59-strand, revealing the 39 overhang needed for recombination. RPA bound to ssDNA also represents a barrier, explaining the need for the motor activity of DNA2 to displace RPA prior to resection. Using ensemble and single molecule biochemistry, we show that phosphorylated CtIP dramatical...
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#1Kristina Kasaciunaite (Leipzig University)H-Index: 2
#2Fergus Fettes (Leipzig University)H-Index: 1
Last. Ralf Seidel (WWU: University of Münster)H-Index: 37
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1 CitationsSource
#2Fergus FettesH-Index: 1
Last. Ralf SeidelH-Index: 37
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DNA double-strand break repair by homologous recombination employs long-range resection of the 59 DNA ends at the break points. In Saccharomyces cerevisiae, this process can be performed by the RecQ helicase Sgs1 and the helicase-nuclease Dna2. Though functional interplay has been shown, it remains unclear whether and how the proteins cooperate on the molecular level. Here, we resolved the dynamics of DNA unwinding by Sgs1 at the single molecule level and investigated its regulation by Dna2, the...
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#2Fergus FettesH-Index: 1
Last. Ralf SeidelH-Index: 37
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#1Felix E. Kemmerich (Leipzig University)H-Index: 4
#2Kristina Kasaciunaite (Leipzig University)H-Index: 2
Last. Ralf Seidel (Leipzig University)H-Index: 37
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Abstract Magnetic tweezers provide a versatile toolkit supporting the mechanistic investigation of helicases. In the present article, we show that custom magnetic tweezers setups are straightforward to construct and can easily be extended to provide adaptable platforms, capable of addressing a multitude of enquiries regarding the functions of these fascinating molecular machines. We first address the fundamental components of a basic magnetic tweezers scheme and review some previous results to d...
9 CitationsSource
#1Cosimo Pinto (UZH: University of Zurich)H-Index: 5
#2Kristina Kasaciunaite (Leipzig University)H-Index: 2
Last. Petr Cejka (UZH: University of Zurich)H-Index: 28
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Human DNA2 (hDNA2) contains both a helicase and a nuclease domain within the same polypeptide. The nuclease of hDNA2 is involved in a variety of DNA metabolic processes. Little is known about the role of the hDNA2 helicase. Using bulk and single-molecule approaches, we show that hDNA2 is a processive helicase capable of unwinding kilobases of dsDNA in length. The nuclease activity prevents the engagement of the helicase by competing for the same substrate, hence prominent DNA unwinding by hDNA2 ...
23 CitationsSource
#1Cosimo Pinto (UZH: University of Zurich)H-Index: 5
#2Kristina Kasaciunaite (Leipzig University)H-Index: 2
Last. Petr Cejka (UZH: University of Zurich)H-Index: 28
view all 4 authors...
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