Jay H. Lewis
Washington Department of Social and Health Services
OutbreakEnterobacteriaceaeEscherichia coliBiologyMicrobiology
What is this?
Publications 12
Abstract Study Objective:To describe the spectrum of illnesses associated withEscherichia coli0157:H7 infections. Design:Described an outbreak that showed the broad spectrum of these infections. Re...
#1Rodrigo G. VillarH-Index: 3
#2Mark D. Macek (CDC: Centers for Disease Control and Prevention)H-Index: 2
Last. Paul S. MeadH-Index: 48
view all 10 authors...
ContextMultidrug-resistant Salmonella Typhimurium DT104 has recently emerged as a cause of human and animal illness in Europe and North America. In early 1997, health officials in Yakima County, Washington, noted a 5-fold increase in salmonellosis among the county's Hispanic population.ObjectivesTo characterize bacterial strains and identify risk factors for infection with Salmonella Typhimurium in Yakima County.DesignLaboratory, case-control, and environmental investigations.Setting and Partici...
136 CitationsSource
#1Cody ShH-Index: 1
#2Glynn MkH-Index: 1
Last. Duc J. VugiaH-Index: 60
view all 13 authors...
Background: Escherichia coli O157:H7 infections have traditionally been associated with animal products, but outbreaks associated with produce have been reported with increasing frequency. In fall 1996, a small cluster of E. coli O157:H7 infections was epidemiologically linked to a particular brand (brand A) of unpasteurized apple juice. Objective: To define the extent of the outbreak, confirm the source, and determine how the apple juice became contaminated. Design: Descriptive epidemiologic st...
343 CitationsSource
#1Peggy S. HayesH-Index: 35
#2K BlomH-Index: 1
Last. B. SwaminathanH-Index: 30
view all 6 authors...
A phenotypic variant of Escherichia coli serotype O157:H7 (G5101) was isolated from a patient with bloody diarrhea. Strain G5101 does not ferment sorbitol but is beta-D-glucuronidase and urease positive. Serotyping and colony hybridization using a serotype-specific DNA probe confirmed that the isolate was O157:H7. G5101 produces Shiga-like toxins I and II and contains an eae gene that is highly conserved in the O157:H7 serotype. This strain would have been missed by laboratories that screen for ...
48 Citations
#1Peggy S. HayesH-Index: 35
Last. Bala SwaminathanH-Index: 31
view all 6 authors...
#1Timothy BarrettH-Index: 53
#2H. LiorH-Index: 2
Last. Patricia M. GriffinH-Index: 70
view all 9 authors...
Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State...
321 Citations
#1Beth P. BellH-Index: 4
#2Marcia GoldoftH-Index: 13
Last. John M. KobayashiH-Index: 11
view all 12 authors...
Objective. —To determine the source of and describe a large outbreak of Escherichia coli O157:H7 infections in Washington State. Design. —Case-control study; environmental investigation; provider-based surveillance for E coli O157:H7 infections. Setting. —Chain of fast-food restaurants, hospitals, physician offices, local laboratories, and local health departments. Participants. —Patients with diarrhea and neighborhood controls. A case was defined as diarrhea with culture-confirmed E coli O157:H...
579 CitationsSource
#1Timothy BarrettH-Index: 53
#2H. LiorH-Index: 2
Last. Jay H. LewisH-Index: 8
view all 8 authors...
strains. ThePFGE patterns of sevenpreoutbreak sporadic isolates andfive sporadic isolates fromtheoutbreak period differed fromthatof theoutbreak strain bya single band,making itdifficult toidentify these isolates asoutbreak ornon-outbreak related. Phagetyping andPFGEwithadditional enzymes werehelpful inresolving this problem. Whilenotas sensitive asPFGE,phagetyping was helpful ininterpreting PFGEdataandcould havebeenusedas asimple, rapid screentoeliminate theneedforperforming PFGEon unrelated is...
2 Citations
#1Stephen M. Ostroff (Washington Department of Social and Health Services)H-Index: 1
#2Phillip I. Tarr (Washington Department of Social and Health Services)H-Index: 2
Last. John M. Kobayashi (Washington Department of Social and Health Services)H-Index: 2
view all 6 authors...
In 1987, 93 Escherichia coli O157:H7 isolates were collected during routine surveillance for this pathogen in the state of Washington. Toxin genotypes and plasmid profiles were correlated with the clinical sequelae of illness in 88 of the 93 patients from whom these strains were isolated. Thirteen plasmid patterns were observed among the 88 tested isolates; four patterns accounted for 82% of the isolates. Genetic probing for Shiga-like toxins (SLT) I and II demonstrated the presence of both gene...
347 CitationsSource
#1Stephen M. OstroffH-Index: 27
#2John KobayashiH-Index: 4
Last. Jay H. LewisH-Index: 8
view all 3 authors...
In 1987, Washington became the first state to require that infection with Escherichia coli serotype 0157:H7 be reported. In the first year of surveillance, 93 cases were reported, yielding an annual incidence of 2.1 cases per 100 000 population. The median age of case patients was 14 years (range, 11 months to 78 years), with the highest attack rate among children younger than 5 years (6.1 cases per 100 000 population per year). Bloody diarrhea was present in 95% of reported cases, 12% of patien...
196 CitationsSource