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Amy Lynd
Liverpool School of Tropical Medicine
25Publications
11H-index
571Citations
Publications 25
Newest
#1Eric R. Lucas (LSTM: Liverpool School of Tropical Medicine)H-Index: 1
#2Kirk A. Rockett (University of Oxford)H-Index: 40
Last.John C. Morgan (LSTM: Liverpool School of Tropical Medicine)H-Index: 12
view all 15 authors...
The spread of resistance to insecticides in disease-carrying mosquitoes poses a threat to the effectiveness of control programmes, which rely largely on insecticide-based interventions. Monitoring mosquito populations is essential, but obtaining phenotypic measurements of resistance is laborious and error-prone. High-throughput genotyping offers the prospect of quick and repeatable estimates of resistance, while also allowing resistance markers to be tracked and studied. To demonstrate the poten...
#1Amy Lynd (LSTM: Liverpool School of Tropical Medicine)H-Index: 11
#2Samuel GonahasaH-Index: 2
Last.Mary KyohereH-Index: 2
view all 13 authors...
Background Long-lasting insecticidal nets (LLINs) are the principal tool for malaria control in Africa and are presently treated with a single class of insecticide; however, increasing levels of insecticide resistance threaten their success. In response to this threat nets have been developed that incorporate the synergist, piperonyl butoxide (PBO), which inhibits the activity of cytochrome P450s which is one main mechanisms of insecticide resistance, allowing resistance to pyrethroids to be rev...
#1Sarah G. Staedke (Lond: University of London)H-Index: 38
#2Moses R. Kamya (MUK: Makerere University)H-Index: 53
Last.Martin J. Donnelly (LSTM: Liverpool School of Tropical Medicine)H-Index: 40
view all 10 authors...
Long-lasting insecticidal nets (LLINs) are a key malaria control intervention, but their effectiveness is threatened by resistance to pyrethroid insecticides. Some new LLINs combine pyrethroids with piperonyl butoxide (PBO), a synergist that can overcome P450-based metabolic resistance to pyrethroids in mosquitoes. In 2017–2018, the Ugandan Ministry of Health distributed LLINs with and without PBO through a national mass-distribution campaign, providing a unique opportunity to rigorously evaluat...
#1Sheila Rugnao (UCSF: University of California, San Francisco)H-Index: 1
#2Samuel GonahasaH-Index: 2
Last.Martin J. Donnelly (LSTM: Liverpool School of Tropical Medicine)H-Index: 40
view all 13 authors...
Background Recent reductions in malaria burden have been attributed largely to long-lasting insecticidal nets (LLINs). In March–June 2017, approximately 3 years after a national LLIN distribution campaign, a cross-sectional community survey was conducted to investigate factors associated with malaria parasitaemia and anaemia, in advance of Uganda’s 2017–2018 LLIN campaign. Methods Households from 104 clusters in 48 districts were randomly selected using two-staged cluster sampling; 50 households...
#1Amy Lynd (LSTM: Liverpool School of Tropical Medicine)H-Index: 11
#2Vasileia Balabanidou (FORTH: Foundation for Research & Technology – Hellas)H-Index: 4
Last.Gareth Lycett (LSTM: Liverpool School of Tropical Medicine)H-Index: 14
view all 7 authors...
Oenocytes are an insect cell type having diverse physiological functions ranging from cuticular hydrocarbon (CHC) production to insecticide detoxification that may impact their capacity to transmit pathogens. To develop functional genetic tools to study Anopheles gambiae oenocytes, we have trapped an oenocyte enhancer to create a transgenic mosquito Gal4 driver line that mediates tissue-specific expression. After crossing with UAS-reporter lines, An. gambiae oenocytes are fluorescently tagged th...
#1Eric R. Lucas (LSTM: Liverpool School of Tropical Medicine)H-Index: 1
#2Kirk A. Rockett (University of Oxford)H-Index: 40
Last.John C. Morgan (LSTM: Liverpool School of Tropical Medicine)H-Index: 12
view all 15 authors...
The spread of resistance to insecticides in the mosquito vectors of diseases such as malaria and dengue poses a threat to the effectiveness of control programmes, which rely largely on insecticide-based interventions. Monitoring the resistance status of mosquito populations is therefore essential, but obtaining direct phenotypic measurements of resistance is laborious and error-prone. In contrast, high-throughput genotyping offers the prospect of quick and repeatable estimates of resistance, whi...
#1Samuel GonahasaH-Index: 2
Last.Janet Hemingway (LSTM: Liverpool School of Tropical Medicine)H-Index: 69
view all 13 authors...
Long-lasting insecticidal nets (LLINs) are a key malaria control intervention. To investigate factors associated with ownership and use of LLINs in Uganda, a cross-sectional community survey was conducted in March–June 2017, approximately 3 years after a national Universal Coverage Campaign (UCC). Households from 104 clusters (health sub-districts) in 48 districts were randomly selected using two-staged cluster sampling; 50 households were enrolled per cluster. Outcomes were household ownership ...
#1Amy Lynd (LSTM: Liverpool School of Tropical Medicine)H-Index: 11
#2Ambrose Oruni (LSTM: Liverpool School of Tropical Medicine)H-Index: 1
Last.David Weetman (LSTM: Liverpool School of Tropical Medicine)H-Index: 26
view all 10 authors...
Background Mutations in the voltage-gated sodium channel at codon 1014 confer knock-down resistance (kdr) to pyrethroids in a wide range of insects. Anopheles gambiae exhibits two mutant alleles at codon 1014, serine and phenylalanine; and both are now widespread across Africa. Existing screening methods only allow for one resistant allele to be detected per assay. A new locked nucleic acid (LNA) qPCR assay was developed for the simultaneous detection of both mutant alleles and the wild type all...
#1Adriana Adolfi (LSTM: Liverpool School of Tropical Medicine)H-Index: 1
#2Emilie Pondeville (Pasteur Institute)H-Index: 7
Last.Gareth Lycett (LSTM: Liverpool School of Tropical Medicine)H-Index: 14
view all 5 authors...
Abstract The ability to manipulate the Anopheles gambiae genome and alter gene expression effectively and reproducibly is a prerequisite for functional genetic analysis and for the development of novel control strategies in this important disease vector. However, in vivo transgenic analysis in mosquitoes is limited by the lack of promoters active ubiquitously. To address this, we used the GAL4/UAS system to investigate the promoter of the An. gambiae Polyubiquitin-c (PUBc) gene and demonstrated ...
#1Alison T. IsaacsH-Index: 4
#2Amy LyndH-Index: 11
Last.Martin J. DonnellyH-Index: 40
view all 3 authors...
Recent successes in malaria control have been largely attributable to the deployment of insecticide-based vector control tools such as bed nets and indoor residual spraying. Pyrethroid-treated bed nets are acutely neurotoxic to mosquitoes, inducing symptoms such as loss of coordination, paralysis, and violent spasms. One result of pyrethroid exposure often seen in laboratory tests is mosquito leg loss, a condition that has thus far been assumed to equate to mortality, as females are not expected...
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