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Reed M. Johnson
Ohio State University
PesticideHoney beeVarroa destructorBotanyBiology
62Publications
26H-index
5,319Citations
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Publications 63
Newest
#1Claudio Vrisman (Ohio Agricultural Research and Development Center)
#1Cláudio Maurício Vrisman (OSU: Ohio State University)H-Index: 2
Last. Sally A. Miller (OSU: Ohio State University)H-Index: 30
view all 6 authors...
Plant pathogenic bacteria in the genus Erwinia cause economically important diseases, including bacterial wilt of cucurbits caused by Erwinia tracheiphila. Conventional bactericides are insufficient to control this disease. Using high-throughput screening, 464 small molecules (SMs) with either cidal or static activity at 100 microM against a cucumber strain of E. tracheiphila were identified. Among them, 20 SMs (SM1 to SM20), composed of nine distinct chemical moiety structures, were cidal to mu...
Source
#1Rodney T. Richardson (York University)H-Index: 5
#2Douglas B. Sponsler (PSU: Pennsylvania State University)H-Index: 4
Last. Reed M. Johnson (OSU: Ohio State University)H-Index: 26
view all 4 authors...
2 CitationsSource
#1Rodney T. Richardson (York University)H-Index: 5
#2Tyler D. Eaton (OSU: Ohio State University)H-Index: 1
Last. Douglas B. SponslerH-Index: 4
view all 6 authors...
Understanding animal foraging ecology requires large samples sizes spanning broad environmental and temporal gradients. For pollinators, this has been hampered by the laborious nature of morphologically identifying pollen. Metagenetic pollen analysis is a solution to this issue, but the field has struggled with poor quantitative performance. Building upon prior laboratory and bioinformatic methods, we applied quantitative multi-locus metabarcoding to characterize the foraging ecology of honey be...
Source
#1Rodney T. Richardson (York University)H-Index: 5
#2Douglas B. Sponsler (PSU: Pennsylvania State University)H-Index: 4
Last. Reed M. Johnson (OSU: Ohio State University)H-Index: 26
view all 4 authors...
The community-level analysis of samples containing diverse genetic material, via metabarcoding and metagenomic approaches, is increasingly popular. While the production of sequence data for such studies has become straightforward, questions remain about how best to analyze and taxonomically characterize sequence data. For many sequence classification approaches, an important component of the workflow involves the curation of reference sequences. Ideally, this involves trimming away extraneous se...
Source
#1Rodney T. Richardson (OSU: Ohio State University)H-Index: 5
#2Hailey R. Curtis (OSU: Ohio State University)H-Index: 1
Last. Reed M. Johnson (OSU: Ohio State University)H-Index: 26
view all 8 authors...
7 CitationsSource
#1Andrea WadeH-Index: 1
#2Chia-Hua LinH-Index: 5
Last. Reed M. JohnsonH-Index: 26
view all 5 authors...
Beekeepers providing pollination services for California almond orchards have reported observing dead or malformed brood during and immediately after almond bloom—effects that they attribute to pesticide exposure. The objective of this study was to test commonly used insecticides and fungicides during almond bloom on honey bee larval development in a laboratory bioassay. In vitro rearing of worker honey bee larvae was performed to test the effect of three insecticides (chlorantraniliprole, diflu...
5 CitationsSource
#1Johan Bengtsson-Palme (UW: University of Wisconsin-Madison)H-Index: 23
#2Rodney T. Richardson (Ohio Agricultural Research and Development Center)H-Index: 5
Last. R. Henrik Nilsson (University of Gothenburg)H-Index: 38
view all 12 authors...
Motivation: Correct taxonomic identification of DNA sequences is central to studies of biodiversity using both shotgun metagenomic and metabarcoding approaches. However, no genetic marker gives sufficient performance across all the biological kingdoms, hampering studies of taxonomic diversity in many groups of organisms. This has led to the adoption of a range of genetic markers for DNA metabarcoding. While many taxonomic classification software tools can be re-trained on these genetic markers, ...
11 CitationsSource
#1Rodney T. Richardson (OSU: Ohio State University)H-Index: 5
#2Hailey R. Curtis (OSU: Ohio State University)H-Index: 1
Last. Reed M. Johnson (OSU: Ohio State University)H-Index: 26
view all 8 authors...
We explored the pollen foraging behavior of honey bee colonies situated in the corn and soybean dominated agroecosystems of central Ohio over a month-long period using both pollen metabarcoding and waggle dance inference of spatial foraging patterns. For molecular pollen analysis we developed simple and cost-effective laboratory and bioinformatics methods. Targeting four plant barcode loci (ITS2, rbcL, trnL and trnH), we implemented metabarcoding library preparation and dual-indexing protocols d...
Source
#1Rodney T. Richardson (OSU: Ohio State University)H-Index: 5
#2Johan Bengtsson-Palme (University of Gothenburg)H-Index: 23
Last. Reed M. Johnson (OSU: Ohio State University)H-Index: 26
view all 4 authors...
5 CitationsSource
#1Reed M. Johnson (Ohio Agricultural Research and Development Center)H-Index: 26
#2Brock A. Harpur (U of T: University of Toronto)H-Index: 12
Last. May R. Berenbaum (UIUC: University of Illinois at Urbana–Champaign)H-Index: 69
view all 5 authors...
Comparative analyses of genomes of ten bee species representing different degrees of social complexity have demonstrated that independent transitions to eusociality are often accompanied by or lead to expansions or contractions of gene families (albeit different families across independent lineages). Because collective gathering, processing, and storage of plant products is a hallmark of bee social complexity, we examined the genomic inventory of cytochrome P450 genes (the CYPome) in these ten g...
6 CitationsSource
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