Match!
A. C. Dock
Centre national de la recherche scientifique
Messenger RNATransfer RNAMoleculeBiochemistryCrystallography
7Publications
5H-index
246Citations
What is this?
Publications 7
Newest
#1Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
#2A. C. Dock (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last. Dino Moras (CNRS: Centre national de la recherche scientifique)H-Index: 85
view all 6 authors...
Abstract In structural biology, the crystallization of the macromolecules often represents the most challenging step. Beside classical factors which determine the solubility of macromolecules, purity of compounds is another major parameter governing crystal growth. With aminoacyl-tRNA synthetases and transfer ribonucleic acids as examples, it will be shown that molecules to be crystallized not only have to be pure in terms of contaminating molecules, but also in terms of sequence integrity and c...
72 CitationsSource
#1Bernard Chevrier (CNRS: Centre national de la recherche scientifique)H-Index: 13
#2A. C. Dock (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last. Eric Westhof (CNRS: Centre national de la recherche scientifique)H-Index: 84
view all 7 authors...
Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at different temperatures (5°C, 18°C and 37°C) in the absence of divalent cations. The crystals grown at 5°C did not diffract X-rays, while those grown at 18°C and 37°C did. The oligomer adopts the left-handed ZI conformation in both crystals. The main difference resides in a more extensive hydration shell in the crystal grown at high temperature than in the crystal grown at low temperature. The high-temperature crystal display...
61 CitationsSource
The crystal structure of yeast tRNAAsp enables visualization of an anticodon-anticodon interaction at the molecular level. Except for differences in the base stacking and twist, the overall conformation of the anticodon loop is quite similar to that of yeast tRNAPhe. The anticodon nucleotide triplets, GUC, of two symmetrically related molecules form a minihelix of the RNA type 11. The modified base m1G37 stacks on both sides of the triplets and enforces the continuity with the anticodon stems. A...
47 CitationsSource
#1Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
#2A. C. Dock (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last. Dino Moras (CNRS: Centre national de la recherche scientifique)H-Index: 85
view all 7 authors...
The three-dimensional structures of two elongator transfer RNAs are known in great details: first that of yeast tRNA(Phe) (1–4) and more recently that of yeast tRNA(Asp) (5–8). As seen in Figure 1, both molecules are folded in an L-shaped conformation, which is also found for initiator tRNAs (9,10). Since the conserved or semi-conserved residues (11) are involved in the tertiary interactions which stabilize this folding (1–10), the L-shaped structure represents the general structural organizatio...
Source
#1Dino Moras (CNRS: Centre national de la recherche scientifique)H-Index: 85
#2A. C. Dock (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last. Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 7 authors...
Abstract The anticodon of yeast tRNAAsp, GUC, presents the peculiarity to be self-complementary, with a slight mismatch at the uridine position. In the orthorhombic crystal lattice, tRNAAsp molecules are associated by anticodon-anticodon interactions through a two-fold symmetry axis. The anticodon triplets of symmetrically related molecules are base paired and stacked in a normal helical conformation. A stacking interaction between the anticodon loops of two two-fold related tRNA molecules also ...
27 CitationsSource
#1A. C. DockH-Index: 5
#2Bernard LorberH-Index: 28
Last. Richard GiegéH-Index: 54
view all 6 authors...
Resume Une compilation des experiences de cristallisation de tRNA publiees dans la litterature ainsi que des resultats originaux sont rassembles et discutes dans cet article. A ce jour 17 tRNAs differents provenant de Escherichia coli et de la levure Saccharomyces cerevisiae ont ete cristallises. Toutes les familles structurales de tRNAs sont representees, c''est-a-dire les tRNAs a grande et a petite boucle variable et parmi ces derniers les tRNAs initiateurs. Les tRNAs a petite boucle variable ...
36 CitationsSource
#1Dino Moras (CNRS: Centre national de la recherche scientifique)H-Index: 85
#2A. C. Dock (CNRS: Centre national de la recherche scientifique)H-Index: 5
Last. Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 6 authors...
The molecular structure of yeast tRNAAsp, a short extra-loop tRNA, was solved at 3 A resolution in two closely related crystal forms. The resulting model confirms the folding originally found in tRNAPhe: major differences concern the conformations of the loops and the relative positioning of the acceptor and anticodon stems which are more open, conferring to the tRNAAsp molecule its boomerang like shape. Crystal packing involves self-complementary GUC anticodon interactions thus making the struc...
3 CitationsSource
1