Rajesh Gupta
Queensland University of Technology
21Publications
7H-index
199Citations
Publications 21
Newest
Published on Dec 1, 2016in Proteome Science 1.77
Saul Chemonges4
Estimated H-index: 4
(University of Queensland),
Rajesh Gupta7
Estimated H-index: 7
(Queensland University of Technology)
+ 2 AuthorsPawel Sadowski9
Estimated H-index: 9
(Queensland University of Technology)
Abstract Background Unlike humans, there is currently no publicly available reference mass spectrometry-based circulating acellular proteome data for sheep, limiting the analysis and interpretation of a range of physiological changes and disease states. The objective of this study was to develop a robust and comprehensive method to characterise the circulating acellular proteome in ovine serum. Methods Serum samples from healthy sheep were subjected to shotgun proteomic analysis using nano liqui...
3 Citations Source Cite
Srihari Sharma (Queensland University of Technology), Rajesh Gupta7
Estimated H-index: 7
(Queensland University of Technology)
+ 4 AuthorsShooter Gary (Queensland University of Technology)
Abstract Introduction Diabetes-related ulcers are a common and severe complication of diabetes which is expected to increase in prevalence in line with projected global growth in rates of diabetes. Caring for these chronic wounds imposes a multi-billion dollar burden on the health care systems. These ulcers can prove lethal if untreated or not recognised and can lead to critical health complications. Methods To investigate underlying causes of wound chronicity, proteomic analyses of swab samples...
Source Cite
Published on Dec 1, 2013in Biochimica et Biophysica Acta 3.44
Manaswini Sivaramakrishnan5
Estimated H-index: 5
(Queensland University of Technology),
Tristan I. Croll14
Estimated H-index: 14
(Queensland University of Technology)
+ 4 AuthorsGary K. Shooter8
Estimated H-index: 8
(Queensland University of Technology)
Numerous studies have reported associations between IGF-I and other extra cellular matrix (ECM) proteins, including fibronectin (FN), integrins, IGF-binding proteins (IGFBPs) and through IGFBPs, with vitronectin (VN). Nevertheless, the precise nature and mechanisms of these interactions are still being characterised. In this paper, we discuss transglutaminases (TGases) as a constituent of the ECM and provide evidence for the first time that IGF-I is a lysine (K)-donor substrate to TGases. When I...
2 Citations Source Cite
Published on Oct 1, 2013in Applied and Environmental Microbiology 3.63
Páraic Ó Cuív14
Estimated H-index: 14
,
Rajesh Gupta7
Estimated H-index: 7
+ 1 AuthorsMark Morrison42
Estimated H-index: 42
(Ohio State University)
Clostridium thermocellum encodes a cellulosomal, modular, and thermostable serine protease inhibitor (serpin), PinA. PinA stability but not inhibitory activity is affected by the Fn(III) and Doc(I) domains, and PinA is a broad inhibitor of subtilisin-like proteases and may play a key role in protecting the cellulosome from protease attack.
5 Citations Source Cite
Published on Aug 15, 2012in Applied and Environmental Microbiology 3.63
Alasdair Mackenzie15
Estimated H-index: 15
(Norwegian University of Life Sciences),
Phillip B. Pope17
Estimated H-index: 17
(Norwegian University of Life Sciences)
+ 4 AuthorsVincentius Gerardus Henricus Eijsink10
Estimated H-index: 10
(Norwegian University of Life Sciences)
We demonstrate that two characteristic Sus-like proteins encoded within a polysaccharide utilization locus (PUL) bind strongly to cellulosic substrates and interact with plant primary cell walls. This shows associations between uncultured Bacteroidetes-affiliated lineages and cellulose in the rumen and thus presents new PUL-derived targets to pursue regarding plant biomass degradation.
26 Citations Source Cite
Published on Mar 9, 2011in Journal of Agricultural and Food Chemistry 3.41
John W. Holland13
Estimated H-index: 13
,
Rajesh Gupta7
Estimated H-index: 7
+ 1 AuthorsPaul F. Alewood55
Estimated H-index: 55
Molecular changes in milk proteins during storage of UHT-treated milk have been investigated using two-dimensional electrophoresis (2-DE) coupled to MALDI-TOF mass spectrometry. UHT-treated samples were stored at three different temperatures, 4 °C, 28 °C, and 40 °C, for two months. Three main changes could be observed on 2-DE gels following storage. They were (1) the appearance of diffuse staining regions above the position of the monomeric caseins caused by nondisulfide cross-linking of α and β...
44 Citations Source Cite
Published on Dec 29, 2008in Protein Science 2.41
Rajesh Gupta7
Estimated H-index: 7
(University of Wollongong),
Samir M. Hamdan6
Estimated H-index: 6
(Australian National University)
+ 2 AuthorsJennifer L. Beck25
Estimated H-index: 25
(University of Wollongong)
Electrospray ionization mass spectrometry (ESI-MS) has widespread routine use as a tool in proteomics for confirmation of primary structure determination and for characterization of purified proteins (Griffiths et al. 2001). Application of ESI-MS to detection and characterization of non-covalent complexes of biomolecules is not as well established, but there are now many examples of noncovalent interactions that have been studied in the gas phase, including those between protein subunits, protei...
22 Citations Source Cite
Published on Feb 1, 2005in Journal of Inorganic Biochemistry 3.06
Rajesh Gupta7
Estimated H-index: 7
(University of Wollongong),
Jennifer L. Beck25
Estimated H-index: 25
(University of Wollongong)
+ 1 AuthorsStephen F. Ralph24
Estimated H-index: 24
(University of Wollongong)
Abstract A combination of enzymatic digestion and electrospray ionisation mass spectrometry (ESI-MS) was used to characterise bifunctional adducts in which cisplatin is bound to GA base sequences in 8mer and 16mer oligonucleotides that do not contain other, higher affinity binding sites. The extent of formation of bifunctional adducts with GA base sequences was significant, but less than that seen with similar oligonucleotides containing either AG or GG sequences.
21 Citations Source Cite
Rajesh Gupta7
Estimated H-index: 7
(University of Wollongong),
Jennifer L. Beck25
Estimated H-index: 25
(University of Wollongong)
+ 2 AuthorsJanice R. Aldrich-Wright27
Estimated H-index: 27
(University of Western Sydney)
Positive and negative ion electrospray ionization (ESI) mass spectra of complexes of positively charged small molecules (distamycin, Hoechst 33258, [Ru(phen)2dpq]Cl2 and [Ru(phen)2dpqC]Cl2) have been compared. [Ru(phen)2dpq]Cl2 and [Ru(phen)2dpqC]Cl2 bind to DNA by intercalation. Negative ion ESI mass spectra of mixtures of [Ru(phen)2dpq]Cl2 or [Ru(phen)2dpqC]Cl2 with DNA showed ions from DNA-ligand complexes consistent with solution studies. In contrast, only ions from free DNA were present in ...
23 Citations Source Cite
Published on Feb 20, 2003in Chemical Communications 6.29
Jennifer L. Beck25
Estimated H-index: 25
(University of Wollongong),
Rajesh Gupta7
Estimated H-index: 7
(University of Wollongong)
+ 4 AuthorsStephen F. Ralph24
Estimated H-index: 24
(University of Wollongong)
ESI mass spectra show that up to five ruthenium molecules can bind non-covalently to double stranded 16mer DNA, and provide information on the relative affinity and DNA sequence selectivity of different ruthenium complexes.
10 Citations Source Cite
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