Rajesh Gupta
Queensland University of Technology
Publications 22
The specific positions of carbon-carbon double bond(s) within an unsaturated fatty acid exert a significant effect on the physical and chemical properties of the lipid that ultimately inform its biological function(s). Contemporary liquid-chromatography mass spectrometry (MS) strategies based on electrospray ionisation coupled to tandem MS can easily detect fatty acyl lipids but generally cannot reveal those specific site(s) of unsaturation. Herein, we describe a novel and versatile workflow whe...
#1Saul Chemonges (UQ: University of Queensland)H-Index: 4
#2Rajesh Gupta (QUT: Queensland University of Technology)H-Index: 7
Last.Pawel Sadowski (QUT: Queensland University of Technology)H-Index: 9
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Abstract Background Unlike humans, there is currently no publicly available reference mass spectrometry-based circulating acellular proteome data for sheep, limiting the analysis and interpretation of a range of physiological changes and disease states. The objective of this study was to develop a robust and comprehensive method to characterise the circulating acellular proteome in ovine serum. Methods Serum samples from healthy sheep were subjected to shotgun proteomic analysis using nano liqui...
#1Srihari Sharma (QUT: Queensland University of Technology)
#2Rajesh Gupta (QUT: Queensland University of Technology)H-Index: 7
Last.Shooter Gary (QUT: Queensland University of Technology)
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Abstract Introduction Diabetes-related ulcers are a common and severe complication of diabetes which is expected to increase in prevalence in line with projected global growth in rates of diabetes. Caring for these chronic wounds imposes a multi-billion dollar burden on the health care systems. These ulcers can prove lethal if untreated or not recognised and can lead to critical health complications. Methods To investigate underlying causes of wound chronicity, proteomic analyses of swab samples...
#1Manaswini Sivaramakrishnan (QUT: Queensland University of Technology)H-Index: 6
#2Tristan I. Croll (QUT: Queensland University of Technology)H-Index: 14
Last.Gary K. Shooter (QUT: Queensland University of Technology)H-Index: 8
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Numerous studies have reported associations between IGF-I and other extra cellular matrix (ECM) proteins, including fibronectin (FN), integrins, IGF-binding proteins (IGFBPs) and through IGFBPs, with vitronectin (VN). Nevertheless, the precise nature and mechanisms of these interactions are still being characterised. In this paper, we discuss transglutaminases (TGases) as a constituent of the ECM and provide evidence for the first time that IGF-I is a lysine (K)-donor substrate to TGases. When I...
#1Páraic Ó CuívH-Index: 15
#2Rajesh GuptaH-Index: 7
Last.Mark Morrison (OSU: Ohio State University)H-Index: 43
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Clostridium thermocellum encodes a cellulosomal, modular, and thermostable serine protease inhibitor (serpin), PinA. PinA stability but not inhibitory activity is affected by the Fn(III) and Doc(I) domains, and PinA is a broad inhibitor of subtilisin-like proteases and may play a key role in protecting the cellulosome from protease attack.
#1Alasdair Mackenzie (NMBU: Norwegian University of Life Sciences)H-Index: 15
#2Phillip B. Pope (NMBU: Norwegian University of Life Sciences)H-Index: 18
Last.Vincentius Gerardus Henricus Eijsink (NMBU: Norwegian University of Life Sciences)H-Index: 11
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We demonstrate that two characteristic Sus-like proteins encoded within a polysaccharide utilization locus (PUL) bind strongly to cellulosic substrates and interact with plant primary cell walls. This shows associations between uncultured Bacteroidetes-affiliated lineages and cellulose in the rumen and thus presents new PUL-derived targets to pursue regarding plant biomass degradation.