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Silvia Martínez-Subiela
University of Murcia
ImmunologyHaptoglobinSalivaAcute-phase proteinBiology
146Publications
24H-index
2,150Citations
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Publications 161
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Summary Point of care (POC) systems for the joint measurement of Troponin and D-dimers have not been studied in horses. The aim of this study was to perform the validation of a POC system (AQT90 FLEX) for the measurement of cardiac troponin I (cTnI) and D-dimers in serum of horses with gastrointestinal diseases. The main objective was to evaluate whether or not this system can distinguish healthy animals from diseased animals. A sample of 33 horses was included in the study: control group (n=10)...
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#1Marina López-Arjona (University of Murcia)H-Index: 2
#2Fernando Tecles (University of Murcia)H-Index: 21
Last. Silvia Martínez-Subiela (University of Murcia)H-Index: 24
view all 7 authors...
Abstract Two sensitive assays based on AlphaLISA technology were developed and validated for the measurement of cortisol and cortisone in hair of pigs, that also enabled estimation of 11β-hydroxysteroid dehydrogenase type 2 activity. These assays were applied to hair samples from sows (n = 32) collected at 5 days before, and at 23 and 59 after farrowing, in reproductive cycles in two different periods: spring-summer (n = 16) and winter-spring (n = 16). The assays were precise (imprecision
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#1José J. CerónH-Index: 33
#2Elsa LamyH-Index: 11
Last. Asta TvarijonaviciuteH-Index: 19
view all 7 authors...
In this report, updated information and future perspectives about the use of saliva as a sample for laboratory analysis of the Covid-19 are highlighted. Saliva can be used for the direct detection of the SARS-CoV-2 virus, the quantification of the specific immunoglobulins produced against it, and for the evaluation of the non-specific, innate immune response of the patient. Moreover, a deeper knowledge of potential changes in the saliva proteome in this disease may allow the identification of ne...
1 CitationsSource
#1M.J. López-Martínez (University of Murcia)
#2Damián Escribano (University of Murcia)H-Index: 12
Last. José J. Cerón (University of Murcia)H-Index: 33
view all 6 authors...
Abstract D-dimer is a peptide found in serum and is derived from the degradation of blood clots. Even though it has been analysed in human saliva, D-dimer has not been previously evaluated in the saliva of any veterinary species, and its source and role remain unknown. The objectives of this research were firstly, to validate the use of an automated method for the measurement of D-dimer in porcine saliva, and secondly, to evaluate whether D-dimer concentration changes in pig saliva after an acut...
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#1Lorena Franco-Martínez (University of Murcia)H-Index: 3
Last. Asta Tvarijonaviciute (University of Murcia)H-Index: 19
view all 9 authors...
The aim of this study was to evaluate changes in serum and saliva proteomes in canine mammary tumors (CMT) using a high-throughput quantitative proteomic analysis in order to potentially discover possible biomarkers of this disease. Proteomes of paired serum and saliva samples from healthy controls (HC group, n = 5) and bitches with CMT (CMT group, n = 5) were analysed using a Tandem Mass Tags-based approach. Twenty-five dogs were used to validate serum albumin as a candidate biomarker in an ind...
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#1Lorena Franco-Martínez (University of Murcia)H-Index: 3
#2Jorge Miguel González-Hernández (UEM: European University of Madrid)
Last. Pedro Jiménez Reyes (URJC: King Juan Carlos University)H-Index: 1
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Abstract The aim of the present study was to evaluate the differences in salivary proteome at rest and in response to an acute exercise in men and women. For this, unstimulated whole saliva samples in rest and after a bout resistance exercise leading to failure (ELF) of both men and women (n = 5 for each sex) were subjected to isobaric Tandem Mass Tags (TMT) labelling followed by LC-MS/MS. A total of 274 proteins were identified and met the inclusion criteria. 16 proteins were modulated for the ...
1 CitationsSource
#1Ana Cantos-Barreda (University of Murcia)H-Index: 3
#2Damián Escribano (University of Murcia)H-Index: 12
Last. Silvia Martínez-Subiela (University of Murcia)H-Index: 24
view all 7 authors...
Abstract In this study, the circadian rhythm of IgG2 and IgA specific antibodies in serum and saliva samples of 6 dogs experimentally infected with Leishmania infantum was assessed. Sampling was performed at 8.00, 12.00, 16.00, 20.00, and 00.00 h on two consecutive days. Anti-Leishmania antibody levels in serum were expressed without any correction, whereas in saliva were shown in different ways: without any correction, adjusted by protein concentration and corrected by the salivary flow rate. N...
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#1Lorena Franco-Martínez (University of Murcia)H-Index: 3
#2Silvia Martínez-Subiela (University of Murcia)H-Index: 24
Last. Asta Tvarijonaviciute (University of Murcia)H-Index: 19
view all 7 authors...
Abstract A biomarker is any measurement reflecting an interaction between a biological system and a potential hazard, which may be chemical, physical, or biological. The One World, One Health concept established that human and animal health and the environmental state are highly interconnected sharing common aspects that can be applied globally in these three components. In this paper, we review how the concept of one-health can be applied to biomarkers of health and welfare, with a special focu...
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