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Helena Berglund
Structural Genomics Consortium
46Publications
22H-index
3,621Citations
Publications 46
Newest
#1Crystall M. D. Swarbrick (CSU: Charles Sturt University)H-Index: 6
#2Noelia Roman (CSU: Charles Sturt University)H-Index: 5
Last.Jade K. Forwood (CSU: Charles Sturt University)H-Index: 22
view all 9 authors...
Acetyl-CoA plays a fundamental role in cell signaling and metabolic pathways, with its cellular levels tightly controlled through reciprocal regulation of enzymes that mediate its synthesis and catabolism. ACOT12, the primary acetyl-CoA thioesterase in the liver of human, mouse, and rat, is responsible for cleavage of the thioester bond within acetyl-CoA, producing acetate and coenzyme A for a range of cellular processes. The enzyme is regulated by ADP and ATP, which is believed to be mediated t...
#1Lionel Trésaugues (SGC: Structural Genomics Consortium)H-Index: 11
#2C. Silvander (SGC: Structural Genomics Consortium)H-Index: 3
Last.Pär Nordlund (KI: Karolinska Institutet)H-Index: 51
view all 9 authors...
Summary SHIP2, OCRL, and INPP5B belong to inositol polyphosphate 5-phophatase subfamilies involved in insulin regulation and Lowes syndrome. The structural basis for membrane recognition, substrate specificity, and regulation of inositol polyphosphate 5-phophatases is still poorly understood. We determined the crystal structures of human SHIP2, OCRL, and INPP5B, the latter in complex with phosphoinositide substrate analogs, which revealed a membrane interaction patch likely to assist in sequeste...
#1Hanna Willander (SLU: Swedish University of Agricultural Sciences)H-Index: 8
#2Glareh Askarieh (University of Oslo)H-Index: 9
Last.Jan Johansson (KI: Karolinska Institutet)H-Index: 29
view all 18 authors...
BRICHOS domains are encoded in > 30 human genes, which are associated with cancer, neurodegeneration, and interstitial lung disease (ILD). The BRICHOS domain from lung surfactant protein C proprotein (proSP-C) is required for membrane insertion of SP-C and has anti-amyloid activity in vitro. Here, we report the 2.1 Å crystal structure of the human proSP-C BRICHOS domain, which, together with molecular dynamics simulations and hydrogen-deuterium exchange mass spectrometry, reveals how BRICHOS dom...
#1M.I. SiponenH-Index: 11
#2M. WisniewskaH-Index: 4
Last.Helena BerglundH-Index: 22
view all 9 authors...
The early B-cell factor (EBF) transcription factors are central regulators of development in several organs and tissues. This protein family shows low sequence similarity to other protein families, which is why structural information for the functional domains of these proteins is crucial to understand their biochemical features. We have used a modular approach to determine the crystal structures of the structured domains in the EBF family. The DNA binding domain reveals a striking resemblance t...
#1A. Magnusdottir (KI: Karolinska Institutet)H-Index: 4
#2Ida Johansson (SGC: Structural Genomics Consortium)H-Index: 10
Last.Helena Berglund (SGC: Structural Genomics Consortium)H-Index: 22
view all 5 authors...
#1M. Hammarstrom (KTH: Royal Institute of Technology)H-Index: 4
#2Niklas Hellgren (KTH: Royal Institute of Technology)H-Index: 25
Last.Torleif Härd (KTH: Royal Institute of Technology)H-Index: 39
view all 5 authors...
A prerequisite for structural genomics and related projects is to standardize the process of gene overexpression and protein solubility screening to enable automation for higher throughput. We have tested a methodology to rapidly subclone a large number of human genes and screen these for expression and protein solubility in Escherichia coli. The methodology, which can be partly automated, was used to compare the effect of six different N-terminal fusion proteins and an N-terminal 6*His tag. As ...
#1Anna-Karin Lundbäck (KI: Karolinska Institutet)H-Index: 4
#2Susanne van den Berg (SGC: Structural Genomics Consortium)H-Index: 12
Last.Said Eshaghi (KI: Karolinska Institutet)H-Index: 14
view all 5 authors...
Tobacco etch virus (TEV) protease is generally used to remove affinity tags from target proteins. It has been reported that some detergents inhibit the activity of this protease, and therefore should be avoided when removing affinity tags from membrane proteins. The aim of this study was to explore and evaluate this further. Hence, affinity tag removal with TEV protease was tested from three membrane proteins (a Pgp synthase and two CorA homologs) in the presence of 16 different detergents commo...
#1Susanne Gräslund (SGC: Structural Genomics Consortium)H-Index: 21
#2J. Sagemark (SGC: Structural Genomics Consortium)H-Index: 4
Last.J. Weigelt (SGC: Structural Genomics Consortium)H-Index: 13
view all 11 authors...
Bacterial over-expression of proteins is a powerful tool to obtain soluble protein amenable to biochemical, biophysical and/or structural characterization. However, it is well established that many recombinant proteins cannot be produced in a soluble form. Several theoretical and empirical methods to improve soluble production have been suggested, although there is to date no universally accepted protocol. This report describes, and quantitatively analyses, a systematic multi-construct approach ...
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