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Yu Kang
Kunming University of Science and Technology
11Publications
8H-index
971Citations
Publications 11
Newest
Published on Jan 15, 2019in BMC Biotechnology 2.60
Chu Chu1
Estimated H-index: 1
(Kunming University of Science and Technology),
Zhaohui Yang1
Estimated H-index: 1
(Kunming University of Science and Technology)
+ 7 AuthorsYuyu Niu15
Estimated H-index: 15
(Kunming University of Science and Technology)
Background Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonst...
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Published on Nov 1, 2018in Cell Reports 8.03
Yu Kang8
Estimated H-index: 8
(Kunming University of Science and Technology),
Zongyong Ai5
Estimated H-index: 5
(Kunming University of Science and Technology)
+ 14 AuthorsBaohua Niu1
Estimated H-index: 1
(Kunming University of Science and Technology)
Summary Monkeys are an optimal model species for developing stem cell therapies. We previously reported generating chimeric cynomolgus monkey fetuses using dome-shaped embryonic stem cells (dESCs). However, conventional primed pluripotent stem cells (pPSCs) lack chimera competency. Here, by altering the media in which injected morulae are cultured, we observed increased survival of cynomolgus monkey primed ESCs, induced PSCs, and somatic cell nuclear transfer-derived ESCs, thereby enabling chime...
1 Citations Source Cite
Published on May 1, 2017in Cell 31.40
Yongchang Chen11
Estimated H-index: 11
(Kunming University of Science and Technology),
Juehua Yu12
Estimated H-index: 12
(Tongji University)
+ 34 AuthorsYu Kang8
Estimated H-index: 8
(Kunming University of Science and Technology)
Gene-editing technologies have made it feasible to create nonhuman primate models for human genetic disorders. Here, we report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys serving as a model for a neurodevelopmental disorder, Rett syndrome (RTT), which is caused by loss-of-function mutations in the human MECP2 gene. Male mutant monkeys were embryonic lethal, reiterating that RTT is a disease of females. Through a battery of behavioral analyses, including prim...
34 Citations Source Cite
Published on Apr 1, 2017in Cell Research 15.39
Fei Gao11
Estimated H-index: 11
,
Yuyu Niu15
Estimated H-index: 15
(Kunming University of Science and Technology)
+ 13 AuthorsJuehua Yu12
Estimated H-index: 12
(UCLA: University of California, Los Angeles)
Abstract Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylatio...
10 Citations Source Cite
Published on Dec 20, 2015in Human Molecular Genetics 4.90
Yu Kang8
Estimated H-index: 8
(Kunming University),
Bo Zheng8
Estimated H-index: 8
(Nanjing Medical University)
+ 13 AuthorsHong Wang9
Estimated H-index: 9
(Kunming University)
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary adrenal insufficiency and incomplete or absent sexual maturation, respectively. The associated defects in spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions from Dax1 knockout mouse models provide only limited insight into AHC/HH disease mechanisms, because mouse models exhibit more extensive abnormalities in...
36 Citations Source Cite
Published on Dec 1, 2015in Scientific Reports 4.12
Hong Wang9
Estimated H-index: 9
,
Tao Tan9
Estimated H-index: 9
+ 10 AuthorsJianpeng Liao1
Estimated H-index: 1
Alcoholic liver disease (ALD) is a significant public health issue with heavy medical and economic burdens. The aetiology of ALD is not yet completely understood. The development of drugs and therapies for ALD is hampered by a lack of suitable animal models that replicate both the histological and metabolic features of human ALD. Here, we characterize a rhesus monkey model of alcohol-induced liver steatosis and hepatic fibrosis that is compatible with the clinical progression of the biochemistry...
2 Citations Source Cite
Published on Jul 1, 2015in Human Molecular Genetics 4.90
Yongchang Chen11
Estimated H-index: 11
(Kunming University of Science and Technology),
Yinghui Zheng3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences)
+ 12 AuthorsRuxiao Xing3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences)
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene or cause the pathology seen in human disease. Addressing these issues is necessary if we are to generate large animal models of human diseases using CRISPR/Cas9. Here we used CRISPR/Cas9 to target the monkey dystrophin gene to create mu...
91 Citations Source Cite
Published on Jul 1, 2015in Cell Stem Cell 23.29
Yongchang Chen11
Estimated H-index: 11
(Kunming University of Science and Technology),
Yuyu Niu15
Estimated H-index: 15
(Kunming University of Science and Technology)
+ 12 AuthorsWei Si10
Estimated H-index: 10
(Kunming University of Science and Technology)
Summary Because of their similarity to humans, non-human primates are important models for studying human disease and developing therapeutic strategies. Establishment of chimeric animals using embryonic stem cells (ESCs) could help with these investigations, but has not so far been achieved. Here, we show that cynomolgus monkey ESCs (cESCs) grown in adjusted culture conditions are able to incorporate into host embryos and develop into chimeras with contribution in all three germ layers and in ge...
54 Citations Source Cite
Published on Apr 15, 2015in Human Molecular Genetics 4.90
Yuyu Niu15
Estimated H-index: 15
(Kunming University of Science and Technology),
Xiangyu Guo9
Estimated H-index: 9
(CAS: Chinese Academy of Sciences)
+ 10 AuthorsShang-Hsun Yang18
Estimated H-index: 18
(NCKU: National Cheng Kung University)
Parkinson's disease (PD) is an age-dependent neurodegenerative disease that can be caused by genetic mutations in α-synuclein (α-syn) or duplication of wild-type α-syn; PD is characterized by the deposition of α-syn aggregates, indicating a gain of toxicity from accumulation of α-syn. Although the major neuropathologic feature of PD is the degeneration of dopaminergic (DA) neurons in the substantia nigra, non-motor symptoms including anxiety, cognitive defect and sleep disorder precede the onset...
35 Citations Source Cite
Published on Mar 1, 2014in Cell Stem Cell 23.29
Hailiang Liu11
Estimated H-index: 11
(Tongji University),
Yongchang Chen11
Estimated H-index: 11
(KIZ: Kunming Institute of Zoology)
+ 29 AuthorsShaoyun Lin1
Estimated H-index: 1
(Tongji University)
Summary Recent advances in gene editing technology have introduced the potential for application of mutagenesis approaches in nonhuman primates to model human development and disease. Here we report successful TALEN-mediated mutagenesis of an X-linked, Rett syndrome (RTT) gene, methyl-CpG binding protein 2 ( MECP2 ), in both rhesus and cynomolgus monkeys. Microinjection of MECP2 -targeting TALEN plasmids into rhesus and cynomolgus zygotes leads to effective gene editing of MECP2 with no detected...
124 Citations Source Cite
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