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Yu Kang
Kunming University of Science and Technology
13Publications
8H-index
1,066Citations
Publications 13
Newest
#1Chu Chu (Kunming University of Science and Technology)H-Index: 1
#2Zhaohui Yang (Kunming University of Science and Technology)H-Index: 17
Last.Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
view all 10 authors...
Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonstrated that ...
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#1Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
#2Nianqin Sun (Kunming University of Science and Technology)H-Index: 1
Last.Tao Tan (Kunming University of Science and Technology)H-Index: 10
view all 24 authors...
The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms underlying this transition. Here, we established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bi...
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#1Dayong Wang (UMMS: University of Massachusetts Medical School)H-Index: 86
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 10 authors...
1 CitationsSource
#1Yu Kang (Kunming University of Science and Technology)H-Index: 8
#2Zongyong Ai (Kunming University of Science and Technology)H-Index: 5
Last.Tianqing Li (Kunming University of Science and Technology)H-Index: 9
view all 17 authors...
Summary Monkeys are an optimal model species for developing stem cell therapies. We previously reported generating chimeric cynomolgus monkey fetuses using dome-shaped embryonic stem cells (dESCs). However, conventional primed pluripotent stem cells (pPSCs) lack chimera competency. Here, by altering the media in which injected morulae are cultured, we observed increased survival of cynomolgus monkey primed ESCs, induced PSCs, and somatic cell nuclear transfer-derived ESCs, thereby enabling chime...
2 CitationsSource
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Juehua Yu (Tongji University)H-Index: 15
Last.Yi Eve Sun (Tongji University)H-Index: 4
view all 37 authors...
Gene-editing technologies have made it feasible to create nonhuman primate models for human genetic disorders. Here, we report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys serving as a model for a neurodevelopmental disorder, Rett syndrome (RTT), which is caused by loss-of-function mutations in the human MECP2 gene. Male mutant monkeys were embryonic lethal, reiterating that RTT is a disease of females. Through a battery of behavioral analyses, including prim...
48 CitationsSource
#1Fei GaoH-Index: 13
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Tao Tan (Kunming University of Science and Technology)H-Index: 10
view all 16 authors...
Abstract Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylatio...
15 CitationsSource
#1Yu Kang (Kunming University of Science and Technology)H-Index: 8
#2Bo Zheng (Nanjing Medical University)H-Index: 9
Last.Xingxu Huang (ShanghaiTech University)H-Index: 19
view all 16 authors...
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary adrenal insufficiency and incomplete or absent sexual maturation, respectively. The associated defects in spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions from Dax1 knockout mouse models provide only limited insight into AHC/HH disease mechanisms, because mouse models exhibit more extensive abnormalities in...
40 CitationsSource
#1Hong WangH-Index: 11
#2Tao TanH-Index: 10
Last.Wei SiH-Index: 12
view all 13 authors...
Alcoholic liver disease (ALD) is a significant public health issue with heavy medical and economic burdens. The aetiology of ALD is not yet completely understood. The development of drugs and therapies for ALD is hampered by a lack of suitable animal models that replicate both the histological and metabolic features of human ALD. Here, we characterize a rhesus monkey model of alcohol-induced liver steatosis and hepatic fibrosis that is compatible with the clinical progression of the biochemistry...
4 CitationsSource
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Yinghui Zheng (CAS: Chinese Academy of Sciences)H-Index: 3
Last.Xiao-Jiang Li (CAS: Chinese Academy of Sciences)H-Index: 62
view all 15 authors...
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene or cause the pathology seen in human disease. Addressing these issues is necessary if we are to generate large animal models of human diseases using CRISPR/Cas9. Here we used CRISPR/Cas9 to target the monkey dystrophin gene to create mu...
101 CitationsSource
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Tianqing Li (Kunming University of Science and Technology)H-Index: 9
view all 15 authors...
Summary Because of their similarity to humans, non-human primates are important models for studying human disease and developing therapeutic strategies. Establishment of chimeric animals using embryonic stem cells (ESCs) could help with these investigations, but has not so far been achieved. Here, we show that cynomolgus monkey ESCs (cESCs) grown in adjusted culture conditions are able to incorporate into host embryos and develop into chimeras with contribution in all three germ layers and in ge...
59 CitationsSource
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