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Yu Kang
Kunming University of Science and Technology
GeneMolecular biologyGeneticsBiologyCRISPR
17Publications
9H-index
1,186Citations
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Publications 17
Newest
#1Ran Yang (KI: Karolinska Institutet)
#2Alexander Goedel (KI: Karolinska Institutet)
Last. Chikai Zhou (KI: Karolinska Institutet)H-Index: 3
view all 17 authors...
The signaling network governing the formation of the primitive streak is well understood in mice, but largely unexplored in primates. Advances in single-cell technology and in vitro embryo culture have enabled to characterize the major cell populations involved. However, a detailed map of this process and insights into its regulatory networks are lacking. Herein, we generated a serial single cell atlas of over 30,000 cells spanning peri-implantation to early primitive streak stages in non-human ...
Source
#1Ting Zhang (Kunming University of Science and Technology)H-Index: 1
#2Yu Kang (Kunming University of Science and Technology)H-Index: 9
Last. Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 12 authors...
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#1Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
#2Nianqin Sun (Kunming University of Science and Technology)H-Index: 2
Last. Tao Tan (Kunming University of Science and Technology)H-Index: 11
view all 24 authors...
The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms underlying this transition. Here, we established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bi...
15 CitationsSource
#1Yu Kang (Kunming University of Science and Technology)H-Index: 9
#1Yu Kang (Kunming University of Science and Technology)H-Index: 1
Last. Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
view all 4 authors...
ABSTRACT Owing to their high similarity to humans, non-human primates (NHPs) provide an exceedingly suitable model for the study of human disease. In this Review, we summarize the history of transgenic NHP models and the progress of CRISPR/Cas9-mediated genome editing in NHPs, from the first proof-of-principle green fluorescent protein-expressing monkeys to sophisticated NHP models of human neurodegenerative disease that accurately phenocopy several complex disease features. We discuss not only ...
7 CitationsSource
#1Dan Wang (UMMS: University of Massachusetts Medical School)H-Index: 24
#1Dayong Wang (UMMS: University of Massachusetts Medical School)H-Index: 90
Last. Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 10 authors...
Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno‐associated viruses (rAAVs). Together with previous reports from the authors of this paper and...
1 CitationsSource
#1Chu Chu (Kunming University of Science and Technology)H-Index: 2
#2Zhaohui Yang (Kunming University of Science and Technology)H-Index: 22
Last. Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
view all 10 authors...
Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonstrated that ...
Source
#1Yu Kang (Kunming University of Science and Technology)H-Index: 9
#2Zongyong Ai (Kunming University of Science and Technology)H-Index: 7
Last. Tianqing Li (Kunming University of Science and Technology)H-Index: 9
view all 17 authors...
Summary Monkeys are an optimal model species for developing stem cell therapies. We previously reported generating chimeric cynomolgus monkey fetuses using dome-shaped embryonic stem cells (dESCs). However, conventional primed pluripotent stem cells (pPSCs) lack chimera competency. Here, by altering the media in which injected morulae are cultured, we observed increased survival of cynomolgus monkey primed ESCs, induced PSCs, and somatic cell nuclear transfer-derived ESCs, thereby enabling chime...
2 CitationsSource
Last. Weizhi JiH-Index: 25
view all 13 authors...
: Intracytoplasmic sperm injection (ICSI) and embryo transfer (ET) in nonhuman primates, e.g. rhesus and cynomolgus monkeys, has been widely used in researches of reproductive and developmental biology, and the success rate has been improved significantly. However, unwanted multiple pregnancy occurs frequently during the ICSI-ET in monkeys, most of which leads to miscarriages. To improve the birth rate of pregnancies and to safeguard health of host and baby monkeys, multifetal pregnancy reductio...
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#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 14
#2Juehua Yu (Tongji University)H-Index: 15
Last. Yi Eve Sun (Tongji University)H-Index: 6
view all 37 authors...
Gene-editing technologies have made it feasible to create nonhuman primate models for human genetic disorders. Here, we report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys serving as a model for a neurodevelopmental disorder, Rett syndrome (RTT), which is caused by loss-of-function mutations in the human MECP2 gene. Male mutant monkeys were embryonic lethal, reiterating that RTT is a disease of females. Through a battery of behavioral analyses, including prim...
48 CitationsSource
#1Fei GaoH-Index: 7
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last. Tao Tan (Kunming University of Science and Technology)H-Index: 11
view all 16 authors...
Abstract Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylatio...
15 CitationsSource
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