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Marco Quarta
Stanford University
28Publications
11H-index
660Citations
Publications 28
Newest
#1Karina H. Nakayama (Cardiovascular Institute of the South)H-Index: 9
#2Marco Quarta (Stanford University)H-Index: 11
Last.Thomas A. Rando (Stanford University)H-Index: 68
view all 11 authors...
Traumatic skeletal muscle injuries cause irreversible tissue damage and impaired revascularization. Engineered muscle is promising for enhancing tissue revascularization and regeneration in injured muscle. Here we fabricated engineered skeletal muscle composed of myotubes interspersed with vascular endothelial cells using spatially patterned scaffolds that induce aligned cellular organization, and then assessed their therapeutic benefit for treatment of murine volumetric muscle loss. Murine skel...
#1Vivianne L. Tawfik (Stanford University)H-Index: 16
#2Marco Quarta (Stanford University)H-Index: 11
Last.J. David Clark (Stanford University)H-Index: 51
view all 9 authors...
Chronic pain and disability after limb injury are major public health problems. One key obstacle to addressing these adverse outcomes is that we do not know when exercise should be initiated or whether the beneficial effects of exercise can be reproduced using pharmacological tools. In these studies, we developed and used a murine model of orthopaedic trauma combining tibia fracture and pin fixation with tibialis anterior muscle damage. Behavioral measures included mechanical nociceptive thresho...
#1Tapash Jay Sarkar (Stanford University)
#2Marco QuartaH-Index: 11
Last.BhutaniNidhi (Stanford University)H-Index: 15
view all 12 authors...
Summary Aging is characterized by a gradual loss of function occurring at the molecular, cellular, tissue and organismal levels 1-3 . At the chromatin level, aging is associated with the progressive accumulation of epigenetic errors that eventually lead to aberrant gene regulation, stem cell exhaustion, senescence, and deregulated cell/tissue homeostasis 3 . The technology of nuclear reprogramming to pluripotency, through over-expression of a small number of transcription factors, can revert bot...
#1Karina H. Nakayama (Cardiovascular Institute of the South)H-Index: 9
#2Cynthia AlcazarH-Index: 3
Last.Ngan F. Huang (Cardiovascular Institute of the South)H-Index: 29
view all 9 authors...
Muscle regeneration can be permanently impaired by traumatic injuries, despite the high regenerative capacity of skeletal muscle. Implantation of engineered biomimetic scaffolds to the site of muscle ablation may serve as an attractive off-the-shelf therapeutic approach. The objective of the study was to histologically assess the therapeutic benefit of a three-dimensional spatially patterned collagen scaffold, in conjunction with rehabilitative exercise, for treatment of volumetric muscle loss. ...
#1Marco QuartaH-Index: 11
#2Melinda J. Cromie Lear (Stanford University)H-Index: 1
Last.Thomas A. Rando (Stanford University)H-Index: 68
view all 6 authors...
Despite the regenerative capacity of muscle, tissue volume is not restored after volumetric muscle loss (VML), perhaps due to a loss-of-structural extracellular matrix. We recently demonstrated the structural and functional restoration of muscle tissue in a mouse model of VML using an engineered “bioconstruct,” comprising an extracellular matrix scaffold (decellularized muscle), muscle stem cells (MuSCs), and muscle-resident cells (MRCs). To test the ability of the cell-based bioconstruct to res...
#1Derrick C. Wan (Stanford University)H-Index: 29
#2Stefanie L. Morgan (Stanford University)H-Index: 5
Last.Star K. Huynh (Stanford University)
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Royal jelly is the queen-maker for the honey bee Apis mellifera, and has cross-species effects on longevity, fertility, and regeneration in mammals. Despite this knowledge, how royal jelly or its components exert their myriad effects has remained poorly understood. Using mouse embryonic stem cells as a platform, here we report that through its major protein component Royalactin, royal jelly can maintain pluripotency by activating a ground-state pluripotency-like gene network. We further identify...
#1Robert N. Judson (UBC: University of British Columbia)H-Index: 9
#2Marco Quarta (Stanford University)H-Index: 11
Last.Mark Hamer (Stanford University)H-Index: 2
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Summary The development of cell therapy for repairing damaged or diseased skeletal muscle has been hindered by the inability to significantly expand immature, transplantable myogenic stem cells (MuSCs) in culture. To overcome this limitation, a deeper understanding of the mechanisms regulating the transition between activated, proliferating MuSCs and differentiation-primed, poorly engrafting progenitors is needed. Here, we show that methyltransferase Setd7 facilitates such transition by regulati...
#1Antoine de Morrée (Stanford University)H-Index: 11
#2Cindy T.J. van Velthoven (Stanford University)H-Index: 5
Last.Thomas A. Rando (Stanford University)H-Index: 68
view all 9 authors...
Tissue regeneration depends on the timely activation of adult stem cells. In skeletal muscle, the adult stem cells maintain a quiescent state and proliferate upon injury. We show that muscle stem cells (MuSCs) use direct translational repression to maintain the quiescent state. High-resolution single-molecule and single-cell analyses demonstrate that quiescent MuSCs express high levels of Myogenic Differentiation 1 (MyoD) transcript in vivo, whereas MyoD protein is absent. RNA pulldowns and cost...
#1Marco QuartaH-Index: 11
#2Melinda J. CromieH-Index: 2
Last.Joseph B. ShragerH-Index: 42
view all 11 authors...
Volumetric muscle loss leads to functional muscle impairment, and current stem cell-based treatments show limited efficacy. Here, the authors generate a stem cell scaffold, implant it in mice, and show that an exercise regimen enhances innervation and restoration of muscl…
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