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Kanury V. S. Rao
International Centre for Genetic Engineering and Biotechnology
92Publications
24H-index
2,008Citations
Publications 92
Newest
#1Shweta Duggal (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
#2Mukul Kumar Midha (Translational Health Science and Technology Institute)H-Index: 4
Last.Kanury V. S. Rao (International Centre for Genetic Engineering and Biotechnology)H-Index: 24
view all 4 authors...
Abstract Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein involved in the signal transduction pathways. This dataset enlists proteins which interact with Grb2 in the presence and absence of a mitogenic stimulus. Grb2 expressing HEK293 cells were cultured in light and heavy labeled SILAC media. Normal lysine and arginine were incorporated as light labels while 8 and 10 Da heavier labels of respective isotopes were used for heavy labeling. While light labeled cells were used to ...
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#1S. K. Duggal (DU: University of Delhi)H-Index: 3
#2Noor Jailkhani (International Centre for Genetic Engineering and Biotechnology)H-Index: 3
Last.Ajay Kumar (Translational Health Science and Technology Institute)H-Index: 9
view all 6 authors...
Cell growth and proliferation are two diverse processes yet always linked. Akt1, a serine/threonine kinase, is a multi-functional protein implicated in regulation of cell growth, survival and proliferation. Though it has a role in G1/S progression, the manner by which Akt1 controls cell cycle and blends cell growth with proliferation is not well explored. In this study, we characterize the Akt1 interactome as the cell cycle progresses from G0 to G1/S and G2 phase. For this, Akt1-overexpressing H...
3 CitationsSource
#1Nutan Gupta (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
#2Shweta Duggal (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
Last.Kanury V. S. Rao (International Centre for Genetic Engineering and Biotechnology)H-Index: 24
view all 5 authors...
Objective Signal transduction not only initiates entry into the cell cycle, but also reprograms the cell’s metabolism. To control abnormalities in cell proliferation, both the aspects should be taken care of, thus pleiotropic signaling molecules are considered as crucial modulators. Considering this, we investigated the role of AKT1 in central carbon metabolism. The role of AKT1 has already been established in the process of cell cycle, but its contribution to the central carbon metabolism is sp...
1 CitationsSource
#1Shweta Duggal (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
#2Noor Jailkhani (MIT: Massachusetts Institute of Technology)H-Index: 3
Last.Ajay Kumar (Translational Health Science and Technology Institute)H-Index: 9
view all 5 authors...
Abstract The Rb protein is a tumor suppressor protein that regulates the key G1S checkpoint consequently blocking the progression of cell cycle into S-phase. Despite its pertinent role in cell cycle regulation, comprehensive information on its interacting partners across cell cycle progression is lacking. Here, we aim to submit a comprehensive set of Rb interactors as the cell progresses from G0 through G1 and S into G2 phase in HEK 293 cell line. Affinity purification of HA-tagged Rb protein al...
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#1Nutan Gupta (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
#2Shweta Duggal (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
Last.Ajay Kumar (International Centre for Genetic Engineering and Biotechnology)H-Index: 9
view all 6 authors...
Abstract Akt1 is a multi-functional protein, implicated in multiple human solid tumors. Pertaining to its key role in cell survival, Akt1 is under focus for development of targeted therapies. Functional diversity of Akt1 is a result of its interactions with other proteins; which changes with changing context. This investigation was designed to capture the dynamics of Akt1 Interactome as a function of its active state. Delineating dynamic changes in association of Akt1 with its interactors could ...
1 CitationsSource
#1Parul Mehrotra (International Centre for Genetic Engineering and Biotechnology)H-Index: 2
#2Kanury V. S. Rao (International Centre for Genetic Engineering and Biotechnology)H-Index: 24
Last.Samrat Chatterjee (Translational Health Science and Technology Institute)H-Index: 13
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Abstract Modulation of host metabolic machinery by Mycobacterium tuberculosis is a well established phenomenon. In our earlier study ( Mehrotra et al., 2014 ), we observed a marked increase in acetyl-CoA levels in cells bearing virulent M. tuberculosis infections compared to host cells harbouring avirulent infections. The difference was observed inspite of similar levels of total host cellular pyruvate in both infection types. The present study aimed in capturing the cause for such a phenomenon ...
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#1Shweta Duggal (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
#2Noor Jailkhani (International Centre for Genetic Engineering and Biotechnology)H-Index: 3
Last.Ajay Kumar (Translational Health Science and Technology Institute)H-Index: 9
view all 5 authors...
Abstract Akt1 is a multi-functional protein implicated in key cellular processes including regulation of proliferation, survival, metabolism and protein synthesis. Its functional diversity results through interactions with other proteins which change with changing context. This study was designed to capture proteins, which interact with Akt1 as the cell cycle progresses from G0 to G1S and then G2 phase. Such an insight might help us understand the role of Akt1 in cell cycle, which as of now is n...
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#1Ajay Kumar (International Centre for Genetic Engineering and Biotechnology)H-Index: 9
#2Shilpa Jamwal (Translational Health Science and Technology Institute)H-Index: 6
Last.Kanury V. S. Rao (International Centre for Genetic Engineering and Biotechnology)H-Index: 24
view all 7 authors...
Here we provide data for SILAC and iTRAQ based hyperplexing combined with BONCAT based click chemistry for selective enrichment of newly synthesized proteins secreted by THP1 macrophages at various time points after infection with four different strains of Mycobacterium tuberculosis. The macrophages were infected with H37Ra, H37Rv, BND433 and JAL2287 strains of M. tuberculosis. Newly-synthesized secreted host proteins were observed, starting from six hours post-infection till 26 h, at 4 h interv...
3 CitationsSource
#1Neha Agrawal (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
Last.Partha P. MajumderH-Index: 42
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Summary Incomplete understanding of mechanisms involved in the host–pathogen interactions constrains our efforts to eliminate tuberculosis. In many individuals, resulting from immune response to mycobacterial infection organised structures called granulomas are formed. To identify host responses that may control at least the early stages of infection, we employed an in vitro granuloma model. Here, human PBMCs were infected with live Mycobacterium tuberculosis in culture, and the appearance of gr...
6 CitationsSource
#1Shilpa JamwalH-Index: 6
#2Parul MehrotraH-Index: 2
Last.Kanury V. S. RaoH-Index: 24
view all 6 authors...
Survival of Mycobacterium tuberculosis (Mtb) within the host macrophage is mediated through pathogen-dependent inhibition of phagosome-lysosome fusion, which enables bacteria to persist within the immature phagosomal compartment. By employing ultrastructural examination of different field isolates supported by biochemical analysis, we found that some of the Mtb strains were in fact poorly adapted for subsistence within endocytic vesicles of infected macrophages. Instead, through a mechanism invo...
34 CitationsSource
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