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Monika Michaelis
University of Bremen
10Publications
2H-index
30Citations
Publications 10
Newest
#1Monika Michaelis (University of Bremen)H-Index: 2
Last.Carole C. PerryH-Index: 46
view all 7 authors...
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#1Karsten Stapelfeldt (University of Bremen)H-Index: 1
#2Stephani Stamboroski (Fraunhofer Society)H-Index: 3
Last.Dorothea Brüggemann (University of Bremen)
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As a key player in blood coagulation and tissue repair, fibrinogen has gained increasing attention to develop nanofibrous biomaterial scaffolds for wound healing. Current techniques to prepare protein nanofibers, like electrospinning or extrusion, are known to induce lasting changes in the protein conformation. Often, such secondary changes are associated with amyloid transitions, which can evoke unwanted disease mechanisms. Starting from our recently introduced technique to self-assemble fibrin...
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#1Monika Michaelis (NTU: Nottingham Trent University)H-Index: 2
#2Nils Hildebrand (University of Bremen)H-Index: 4
Last.L. Colombi Ciacchi (University of Bremen)H-Index: 1
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While successful in the structural determination of ordered biomolecules, the spectroscopic investigation of oligopeptides in solution is hindered by their complex and rapidly changing conformational ensemble. The measured circular dichroism (CD) spectrum of an oligopeptide is an ensemble average over all microstates, severely limiting its interpretation, in contrast to ordered biomolecules. Spectral deconvolution methods to estimate the secondary structure contributions in the ensemble are stil...
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#1Daniel Carmona (University of Bremen)H-Index: 1
#2Laura TreccaniH-Index: 18
Last.Michael Maas (University of Bremen)H-Index: 12
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The iron storage protein ferritin is well known for its ability to mineralize iron oxides in its interior cavity. In this study, the authors investigated the mineralization behavior of H and L ferr...
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#1Daniel J. Oliver (NTU: Nottingham Trent University)H-Index: 1
#2Monika Michaelis (NTU: Nottingham Trent University)H-Index: 2
Last.Carole C. Perry (NTU: Nottingham Trent University)H-Index: 46
view all 5 authors...
Polypeptide based biosilica composites show promise as next generation multi-functional nano-platforms for diagnostics and bio-catalytic applications. Following the identification of a strong silica binder (LDHSLHS) by phage display, we conduct structural analysis of the polypeptide at the interface with amorphous silica nanoparticles in an aqueous environment. Our approach relies on modelling infrared and Raman spectral responses using predictions of molecular dynamics simulations and quantum s...
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#1Nils Hildebrand (University of Bremen)H-Index: 4
#2Monika Michaelis (University of Bremen)H-Index: 2
Last.Lucio Colombi Ciacchi (University of Bremen)H-Index: 22
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Adsorption of enzymes on solid surfaces may lead to conformational changes that reduce their catalytic conversion activity and are thus detrimental to the efficiency of biotechnology or biosensing applications. This work is a joint theoretical and experimental endeavor in which we identify and quantify the conformational changes that chymotrypsin undergoes when in contact with the surface of amorphous silica nanoparticles. For this purpose, we use circular dichroism spectroscopy, standard molecu...
1 CitationsSource
#1Anna Sola-Rabada (NTU: Nottingham Trent University)H-Index: 4
#2Monika Michaelis (NTU: Nottingham Trent University)H-Index: 2
Last.Carole C. Perry (NTU: Nottingham Trent University)H-Index: 46
view all 7 authors...
In this contribution, the effect of silica particle size (28 and 210 nm) and surface chemistry (i.e., hydroxyl, methyl, or amino groups) on peptide binding response is studied with a specific emphasis on the effect of the extent of functionalization on binding. Exhaustive characterization of the silica surfaces was crucial for knowledge of the chemistry and topography of the solid surface under study and, thus, to understand their impact on adsorption and the conformational ensemble of the pepti...
2 CitationsSource
#1Fakir Shahidullah Tareq (University of Bremen)H-Index: 1
#2Choudhury M. Hasan (DU: University of Dhaka)H-Index: 18
Last.Peter Spiteller (University of Bremen)H-Index: 21
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Three new and seven known calopins were isolated from Caloboletus radicans. The structures of the new cyclocalopins, 8-deacetylcyclocalopin B (1), cyclocalopin A-15-ol (2), and 12,15-dimethoxycyclocalopin A (3), were mainly elucidated by NMR and MS data analysis. The stereochemistry of 1–3 was assigned based on NOE correlations and coupling constants and by comparison of their CD spectra with those of similar known calopins. While 1–10 were inactive against two cancer cell lines, they displayed ...
1 CitationsSource
Zinc oxide (ZnO) is of widespread use for numerous applications, including many in the cosmetic industry. Thus, ZnO particles are quite likely to enter the environment. ZnO may be harmful because of the release of cytotoxic Zn2+ ions during dissolution reactions. Here, we analyze the dissolution kinetics of the polar zinc-terminated (000–1) and nonpolar (10–10) crystal surfaces in ultrapure water to examine the impact of the crystal defects on dissolution. By using a complementary approach of at...
9 CitationsSource
#1Qing Li (University of Bremen)H-Index: 10
#2Monika Michaelis (University of Bremen)H-Index: 2
Last.Lucio Colombi Ciacchi (University of Bremen)H-Index: 22
view all 4 authors...
We have developed a novel aptasensor based on single-molecule force spectroscopy (SMFS) capable of detecting mercury ions (Hg2+) with sub-nM sensitivity. The single-strand (ss) DNA aptamer used in this work is rich in thymine (T) and readily forms T–Hg2+–T complexes in the presence of Hg2+. The aptamer was conjugated to an atomic force microscope (AFM) probe, and the adhesion force between the probe and a flat graphite surface was measured by single-molecule force spectroscopy (SMFS). The presen...
17 CitationsSource
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