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Junghyun Ryu
Virginia Tech
12Publications
5H-index
56Citations
Publications 12
Newest
#1Kayla Carey (VT: Virginia Tech)
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 7 authors...
Background The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the efficiency of producing genetically modified animal models and having potential clinical applications as an assisted reproductive technology. Because most of the CRISPR/Cas9 systems introduce site-specific double-stranded breaks (DSBs) to induce site-specific modifications, a major co...
#1K. Uh (VT: Virginia Tech)
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 6 authors...
Abstract Artificial oocyte activation is an essential step in somatic cell nuclear transfer (SCNT) and can enhance viability of embryos as a form of assisted reproductive technology (ART) in clinics. Most artificial activation methods have been developed to increase cytosolic calcium (Ca 2+ ) level in oocytes. Interestingly, recent studies have demonstrated that mammalian oocytes can be activated using N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a Zn 2+ chelator. Although effectiv...
#1K. Carey (VT: Virginia Tech)
#2K. Uh (VT: Virginia Tech)
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 4 authors...
Despite extensive efforts, cellular reprogramming in livestock species has had limited success. Induced pluripotent stem cells (iPSC) have been established; however, these cells often show incomplete reprogramming status, and constitutive expression of exogenous reprogramming factors is required due to inactivation of endogenous pluripotency-related genes. A previous study reported that overexpression of the Xenopus egg-derived WAVE1 gene assists reprogramming of murine somatic cells into the pl...
#1K. Uh (VT: Virginia Tech)
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 5 authors...
Ten-eleven translocation (TET) methylcytosine dioxygenases are considered to play an important role in regulation of DNA methylation patterns by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). TET3 protein, a member of TET family, is enriched in mature oocytes and early stage embryos and contributes to DNA demethylation of the paternal genome in zygotes. N-terminal CXXC domain of TET3 is thought to be important in catalysing 5mC oxidation through its DNA binding potential. H...
#1Junghyun Ryu (VT: Virginia Tech)H-Index: 5
#2Randall S. Prather (MU: University of Missouri)H-Index: 64
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 3 authors...
Pigs are an important resource in agriculture and serve as a model for human diseases. Due to their physiological and anatomical similarities with humans, pigs can recapitulate symptoms of human diseases, making them a useful model in biomedicine. However, in the past pig models have not been widely used partially because of the difficulty in genetic modification. The lack of true embryonic stem cells in pigs forced researchers to utilize genetic modification in somatic cells and somatic cell nu...
#1K. Uh (VT: Virginia Tech)
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kiho Lee (VT: Virginia Tech)H-Index: 6
view all 3 authors...
DNA methylation is one of the principal epigenetic modifications playing an essential role in regulating gene expression. The TET family (1-3) is implicated in initiating the demethylation process by converting 5-methylcytosine (5mC) to 5-hydroxymethyl cytosine (5hmC) during embryogenesis. Previous studies in mice suggest that TET1 is required for pluripotency and maintenance of embryonic stem cells by managing their epigenetic marks, specifically DNA methylation. This raises the possibility tha...
#1Jung-Taek KangH-Index: 13
#2Junghyun Ryu (VT: Virginia Tech)H-Index: 5
Last.Kwang-Wook Park (SNU: Sunchon National University)H-Index: 23
view all 10 authors...
Contents Pigs are an attractive animal model to study the progression of cancer because of their anatomical and physiological similarities to human. However, the use of pig models for cancer research has been limited by availability of genetically engineered pigs which can recapitulate human cancer progression. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, specific CRISPR/Cas9 systems were...
#1Jung-Taek KangH-Index: 1
#2Bumrae ChoH-Index: 19
Last.Nathaniel Jue (VT: Virginia Tech)H-Index: 1
view all 13 authors...
Background Pigs with SCID can be a useful model in regenerative medicine, xenotransplantation, and cancer cell transplantation studies. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, we report generation and phenotypic characterization of IL2RG knockout female pigs produced through combination of CRISPR/Cas9 system and SCNT. As expected, pigs lacking IL2RG presented SCID phenotype.
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