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Junghyun Ryu
Virginia Tech
12Publications
5H-index
56Citations
Publications 12
Newest
Published on Dec 1, 2019in BMC Biotechnology2.30
Kayla Carey (VT: Virginia Tech), Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech)
+ 4 AuthorsKiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
Background The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the efficiency of producing genetically modified animal models and having potential clinical applications as an assisted reproductive technology. Because most of the CRISPR/Cas9 systems introduce site-specific double-stranded breaks (DSBs) to induce site-specific modifications, a major co...
Published on Feb 1, 2019in Theriogenology2.30
K. Uh (VT: Virginia Tech), Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech)
+ 3 AuthorsKiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
Abstract Artificial oocyte activation is an essential step in somatic cell nuclear transfer (SCNT) and can enhance viability of embryos as a form of assisted reproductive technology (ART) in clinics. Most artificial activation methods have been developed to increase cytosolic calcium (Ca 2+ ) level in oocytes. Interestingly, recent studies have demonstrated that mammalian oocytes can be activated using N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a Zn 2+ chelator. Although effectiv...
Published on Jan 1, 2019in Reproduction, Fertility and Development1.72
K. Carey (VT: Virginia Tech), K. Uh (VT: Virginia Tech)+ 1 AuthorsKiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
Despite extensive efforts, cellular reprogramming in livestock species has had limited success. Induced pluripotent stem cells (iPSC) have been established; however, these cells often show incomplete reprogramming status, and constitutive expression of exogenous reprogramming factors is required due to inactivation of endogenous pluripotency-related genes. A previous study reported that overexpression of the Xenopus egg-derived WAVE1 gene assists reprogramming of murine somatic cells into the pl...
Published on Jan 1, 2019in Reproduction, Fertility and Development1.72
K. Uh (VT: Virginia Tech), Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech)
+ 2 AuthorsKiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
Ten-eleven translocation (TET) methylcytosine dioxygenases are considered to play an important role in regulation of DNA methylation patterns by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). TET3 protein, a member of TET family, is enriched in mature oocytes and early stage embryos and contributes to DNA demethylation of the paternal genome in zygotes. N-terminal CXXC domain of TET3 is thought to be important in catalysing 5mC oxidation through its DNA binding potential. H...
Published on Dec 1, 2018in Journal of animal science and biotechnology3.44
Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech),
Randall S. Prather64
Estimated H-index: 64
(MU: University of Missouri),
Kiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
Pigs are an important resource in agriculture and serve as a model for human diseases. Due to their physiological and anatomical similarities with humans, pigs can recapitulate symptoms of human diseases, making them a useful model in biomedicine. However, in the past pig models have not been widely used partially because of the difficulty in genetic modification. The lack of true embryonic stem cells in pigs forced researchers to utilize genetic modification in somatic cells and somatic cell nu...
Published on Jan 1, 2018in Reproduction, Fertility and Development1.72
K. Uh (VT: Virginia Tech), Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech),
Kiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
DNA methylation is one of the principal epigenetic modifications playing an essential role in regulating gene expression. The TET family (1-3) is implicated in initiating the demethylation process by converting 5-methylcytosine (5mC) to 5-hydroxymethyl cytosine (5hmC) during embryogenesis. Previous studies in mice suggest that TET1 is required for pluripotency and maintenance of embryonic stem cells by managing their epigenetic marks, specifically DNA methylation. This raises the possibility tha...
Published on Jan 1, 2017in Methods of Molecular Biology
Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech),
Kiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
Published on Dec 1, 2016in Reproduction in Domestic Animals1.64
Jung-Taek Kang13
Estimated H-index: 13
,
Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech)
+ 7 AuthorsKwang-Wook Park23
Estimated H-index: 23
(SNU: Sunchon National University)
Contents Pigs are an attractive animal model to study the progression of cancer because of their anatomical and physiological similarities to human. However, the use of pig models for cancer research has been limited by availability of genetically engineered pigs which can recapitulate human cancer progression. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, specific CRISPR/Cas9 systems were...
Published on Dec 1, 2016in Reproductive Biology and Endocrinology2.59
Jung-Taek Kang1
Estimated H-index: 1
,
Bumrae Cho19
Estimated H-index: 19
+ 10 AuthorsNathaniel Jue1
Estimated H-index: 1
(VT: Virginia Tech)
Background Pigs with SCID can be a useful model in regenerative medicine, xenotransplantation, and cancer cell transplantation studies. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, we report generation and phenotypic characterization of IL2RG knockout female pigs produced through combination of CRISPR/Cas9 system and SCNT. As expected, pigs lacking IL2RG presented SCID phenotype.
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