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Jodi L. Boer
Michigan State University
Binding siteChemistryUreaseActive siteBiochemistry
7Publications
6H-index
310Citations
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Publications 7
Newest
#1Vlad Martin-Diaconescu (UMass: University of Massachusetts Amherst)H-Index: 15
#2Crisjoe Joseph (UMass: University of Massachusetts Amherst)H-Index: 6
Last. Michael J. Maroney (UMass: University of Massachusetts Amherst)H-Index: 40
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Nickel-dependent ureases are activated by a multiprotein complex that includes the GTPase UreG. Prior studies showed that nucleotide-free UreG from Klebsiella aerogenes is monomeric and binds one nickel or zinc ion with near-equivalent affinity using an undefined binding site, whereas nucleotide-free UreG from Helicobacter pylori selectively binds one zinc ion per dimer via a universally conserved Cys-Pro-His motif in each protomer. Iodoacetamide-treated K. aerogenes UreG was nearly unaffected i...
1 CitationsSource
#1Jodi L. Boer (MSU: Michigan State University)H-Index: 6
#2Scott B. Mulrooney (MSU: Michigan State University)H-Index: 17
Last. Robert P. Hausinger (MSU: Michigan State University)H-Index: 53
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This review describes the functions, structures, and mechanisms of nine nickel-containing enzymes: glyoxalase I, acireductone dioxygenase, urease, superoxide dismutase, [NiFe]-hydrogenase, carbon monoxide dehydrogenase, acetyl-coenzyme A synthase/decarbonylase, methyl-coenzyme M reductase, and lactate racemase. These enzymes catalyze their various chemistries by using metallocenters of diverse structures, including mononuclear nickel, dinuclear nickel, nickel–iron heterodinuclear sites, more com...
112 CitationsSource
#1Mark A. Farrugia (MSU: Michigan State University)H-Index: 5
#2Linjie Han (UM: University of Michigan)H-Index: 9
Last. Robert P. Hausinger (MSU: Michigan State University)H-Index: 53
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Maturation of the nickel-containing urease of Klebsiella aerogenes is facilitated by the UreD, UreF, and UreG accessory proteins along with the UreE metallo-chaperone. A fusion of the maltose binding protein and UreD (MBP-UreD) was co-isolated with UreF and UreG in a soluble complex possessing a (MBP-UreD:UreF:UreG)2 quaternary structure. Within this complex a UreF:UreF interaction was identified by chemical cross-linking of the amino termini of its two UreF protomers, as shown by mass spectrome...
9 CitationsSource
#1Jodi L. Boer (MSU: Michigan State University)H-Index: 6
#2Robert P. HausingerH-Index: 53
Urease, an enzyme that is widely found in bacteria, plants, and fungi, hydrolyzes urea into ammonia and carbonic acid, with the resulting ammonification and increase in pH having important implications in medicine and agriculture (1–4). The best-studied urease system is produced by recombinant Escherichia coli expressing the Klebsiella aerogenes urease genes, ureDABCEFG. K. aerogenes urease contains three subunits, UreA, UreB, and UreC, that form a trimer of trimers in the supramolecular structu...
19 CitationsSource
#1Eric L. Carter (MSU: Michigan State University)H-Index: 5
#2Jodi L. Boer (MSU: Michigan State University)H-Index: 6
Last. Robert P. HausingerH-Index: 53
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Urease from Klebsiella aerogenes is composed of three subunits (UreA–UreB–UreC) that assemble into a (UreABC)3 quaternary structure. UreC harbors the dinuclear nickel active site, whereas the functions of UreA and UreB remain unknown. UreD and UreF accessory proteins previously were suggested to reposition UreB and increase the level of exposure of the nascent urease active site, thus facilitating metallocenter assembly. In this study, cells were engineered to separately produce (UreAC)3 or UreB...
11 CitationsSource
#1Jodi L. Boer (MSU: Michigan State University)H-Index: 6
#2Soledad Quiroz-Valenzuela (MSU: Michigan State University)H-Index: 2
Last. Robert P. HausingerH-Index: 53
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UreG is a GTPase required for assembly of the nickel-containing active site of urease. Herein, a Strep-tagged Klebsiella aerogenes UreG (UreGStr) and selected site-directed variants of UreGStr were constructed for studying the in vivo effects on urease activation in recombinant Escherichia coli cells, characterizing properties of the purified proteins, and analysis of in vivo and in vitro protein−protein interactions. Whereas the Strep tag had no effect on UreG’s ability to activate urease, enzy...
38 CitationsSource
#1Eric L. Carter (MSU: Michigan State University)H-Index: 5
#2Nicholas Flugga (MSU: Michigan State University)H-Index: 2
Last. Robert P. Hausinger (MSU: Michigan State University)H-Index: 53
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Urease, the first enzyme to be crystallized, contains a dinuclear nickel metallocenter that catalyzes the decomposition of urea to produce ammonia, a reaction of great agricultural and medical importance. Several mechanisms of urease catalysis have been proposed on the basis of enzyme crystal structures, model complexes, and computational efforts, but the precise steps in catalysis and the requirement of nickel versus other metals remain unclear. Purified bacterial urease is partially activated ...
98 CitationsSource
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