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Xiaowei Xie
Sun Yat-sen University
9Publications
7H-index
638Citations
Publications 9
Newest
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Xiaowei Xie (SYSU: Sun Yat-sen University)H-Index: 7
Last.Zhou SongyangH-Index: 45
view all 12 authors...
The adenine base editor (ABE), capable of catalyzing A•T to G•C conversions, is an important gene editing toolbox. Here, we systematically evaluate genome-wide off-target deamination by ABEs using the EndoV-seq platform we developed. EndoV-seq utilizes Endonuclease V to nick the inosine-containing DNA strand of genomic DNA deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to identify off-target sites. Of the eight gRNAs we tested with ABE, 2–19 (with an average of 8.0) o...
10 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Hongwei Sun (SYSU: Sun Yat-sen University)H-Index: 5
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 13 authors...
4 CitationsSource
#1Yuanyan Xiong (SYSU: Sun Yat-sen University)H-Index: 7
#2Xiaowei Xie (SYSU: Sun Yat-sen University)H-Index: 7
Last.Zhiming Dai (SYSU: Sun Yat-sen University)H-Index: 6
view all 7 authors...
The CRISPR/Cas System has been shown to be an efficient and accurate genome-editing technique. There exist a number of tools to design the guide RNA sequences and predict potential off-target sites. However, most of the existing computational tools on gRNA design are restricted to small deletions. To address this issue, we present pgRNAFinder, with an easy-to-use web interface, which enables researchers to design single or distance-free paired-gRNA sequences. The web interface of pgRNAFinder con...
1 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Chenhui Ding (SYSU: Sun Yat-sen University)H-Index: 14
Last.Junjiu Huang (SYSU: Sun Yat-sen University)H-Index: 19
view all 16 authors...
β-Thalassemia is a global health issue, caused by mutations in the HBB gene. Among these mutations, HBB −28 (A>G) mutations is one of the three most common mutations in China and Southeast Asia patients with β-thalassemia. Correcting this mutation in human embryos may prevent the disease being passed onto future generations and cure anemia. Here we report the first study using base editor (BE) system to correct disease mutant in human embryos. Firstly, we produced a 293T cell line with an exogen...
53 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Hongwei Sun (SYSU: Sun Yat-sen University)H-Index: 5
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 14 authors...
Targeted point mutagenesis through homologous recombination has been widely used in genetic studies and holds considerable promise for repairing disease-causing mutations in patients. However, problems such as mosaicism and low mutagenesis efficiency continue to pose challenges to clinical application of such approaches. Recently, a base editor (BE) system built on cytidine (C) deaminase and CRISPR/Cas9 technology was developed as an alternative method for targeted point mutagenesis in plant, ye...
30 CitationsSource
#1Xiya Zhang (SYSU: Sun Yat-sen University)H-Index: 8
#2Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
Last.Junjiu Huang (SYSU: Sun Yat-sen University)H-Index: 19
view all 13 authors...
The CRISPR/Cas system is an efficient genome-editing tool to modify genes in mouse zygotes. However, only the Streptococcus pyogenes Cas9 (SpCas9) has been systematically tested for generating gene-modified mice. The protospacer adjacent motif (PAM, 5′-NGG-3′) recognized by SpCas9 limits the number of potential target sites for this system. Staphylococcus aureus Cas9 (SaCas9), with its smaller size and unique PAM (5′-NNGRRT-3′) preferences, presents an alternative for genome editing in zygotes. ...
17 CitationsSource
#1Xiaowei Xie (SYSU: Sun Yat-sen University)H-Index: 7
#2Wenbin Ma (SYSU: Sun Yat-sen University)H-Index: 12
Last.Yuanyan Xiong (CMU: Carnegie Mellon University)H-Index: 7
view all 7 authors...
Distal regulatory elements have been shown to regulate gene transcription through spatial interactions, and single nucleotide polymorphisms (SNPs) are linked with distal gene expression by spatial proximity, which helps to explain the causal role of disease-associated SNPs in non-coding region. Therefore, studies on spatial interactions between chromatin have created a new avenue for elucidating the mechanism of transcriptional regulation in disease pathogenesis. Recently, a growing number of ch...
11 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Yanwen Xu (SYSU: Sun Yat-sen University)H-Index: 15
Last.Junjiu Huang (SYSU: Sun Yat-sen University)H-Index: 19
view all 16 authors...
Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious knowledge gap remains in our understanding of DNA repair mechanisms in human early embryos, and in the efficiency and potential off-target effects of using technologies such as CRISP...
505 CitationsSource
#1Zhenhua LuoH-Index: 6
#2Zhiming DaiH-Index: 6
Last.Yuanyan XiongH-Index: 7
view all 7 authors...
Interaction network surrounding telomeres has been intensively studied during the past two decades. However, no specific resource by integrating telomere interaction information data is currently available. To facilitate the understanding of the molecular interaction network by which telomeres are associated with biological process and diseases, we have developed TeloPIN (Telomeric Proteins Interaction Network) database (http://songyanglab.sysu.edu.cn/telopin/), a novel database that points to p...
7 CitationsSource
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