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Xiaowei Xie
Sun Yat-sen University
9Publications
6H-index
557Citations
Publications 9
Newest
Published on Jan 8, 2019in Nature Communications 12.35
Puping Liang7
Estimated H-index: 7
(SYSU: Sun Yat-sen University),
Xiaowei Xie6
Estimated H-index: 6
(SYSU: Sun Yat-sen University)
+ 9 AuthorsDan Liu26
Estimated H-index: 26
(BCM: Baylor College of Medicine)
The adenine base editor (ABE), capable of catalyzing A•T to G•C conversions, is an important gene editing toolbox. Here, we systematically evaluate genome-wide off-target deamination by ABEs using the EndoV-seq platform we developed. EndoV-seq utilizes Endonuclease V to nick the inosine-containing DNA strand of genomic DNA deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to identify off-target sites. Of the eight gRNAs we tested with ABE, 2–19 (with an average of 8.0) o...
7 Citations Source Cite
Published on Sep 1, 2018in Protein & Cell 6.23
Puping Liang7
Estimated H-index: 7
(SYSU: Sun Yat-sen University),
Hongwei Sun4
Estimated H-index: 4
(SYSU: Sun Yat-sen University)
+ 10 AuthorsWenbin Ma12
Estimated H-index: 12
(SYSU: Sun Yat-sen University)
2 Citations Source Cite
Published on Nov 15, 2017in Bioinformatics 5.48
Yuanyan Xiong6
Estimated H-index: 6
(SYSU: Sun Yat-sen University),
Xiaowei Xie6
Estimated H-index: 6
(SYSU: Sun Yat-sen University)
+ 4 AuthorsZhiming Dai6
Estimated H-index: 6
(SYSU: Sun Yat-sen University)
1 Citations Source Cite
Published on Nov 1, 2017in Protein & Cell 6.23
Puping Liang7
Estimated H-index: 7
(SYSU: Sun Yat-sen University),
Chenhui Ding14
Estimated H-index: 14
(SYSU: Sun Yat-sen University)
+ 13 AuthorsYongxiang Liu1
Estimated H-index: 1
(SYSU: Sun Yat-sen University)
β-Thalassemia is a global health issue, caused by mutations in the HBB gene. Among these mutations, HBB −28 (A>G) mutations is one of the three most common mutations in China and Southeast Asia patients with β-thalassemia. Correcting this mutation in human embryos may prevent the disease being passed onto future generations and cure anemia. Here we report the first study using base editor (BE) system to correct disease mutant in human embryos. Firstly, we produced a 293T cell line with an exogen...
38 Citations Source Cite
Published on Aug 1, 2017in Protein & Cell 6.23
Puping Liang7
Estimated H-index: 7
(SYSU: Sun Yat-sen University),
Hongwei Sun4
Estimated H-index: 4
(SYSU: Sun Yat-sen University)
+ 11 AuthorsYuanyan Xiong6
Estimated H-index: 6
(SYSU: Sun Yat-sen University)
Targeted point mutagenesis through homologous recombination has been widely used in genetic studies and holds considerable promise for repairing disease-causing mutations in patients. However, problems such as mosaicism and low mutagenesis efficiency continue to pose challenges to clinical application of such approaches. Recently, a base editor (BE) system built on cytidine (C) deaminase and CRISPR/Cas9 technology was developed as an alternative method for targeted point mutagenesis in plant, ye...
23 Citations Source Cite
Published on Sep 1, 2016in Scientific Reports 4.12
Xiya Zhang7
Estimated H-index: 7
,
Puping Liang7
Estimated H-index: 7
+ 10 AuthorsJinran Zhang2
Estimated H-index: 2
The CRISPR/Cas system is an efficient genome-editing tool to modify genes in mouse zygotes. However, only the Streptococcus pyogenes Cas9 (SpCas9) has been systematically tested for generating gene-modified mice. The protospacer adjacent motif (PAM, 5′-NGG-3′) recognized by SpCas9 limits the number of potential target sites for this system. Staphylococcus aureus Cas9 (SaCas9), with its smaller size and unique PAM (5′-NNGRRT-3′) preferences, presents an alternative for genome editing in zygotes. ...
16 Citations Source Cite
Published on Jan 1, 2016in Database 3.98
Xiaowei Xie6
Estimated H-index: 6
(SYSU: Sun Yat-sen University),
Wenbin Ma12
Estimated H-index: 12
(SYSU: Sun Yat-sen University)
+ 4 AuthorsYuanyan Xiong6
Estimated H-index: 6
(CMU: Carnegie Mellon University)
Distal regulatory elements have been shown to regulate gene transcription through spatial interactions, and single nucleotide polymorphisms (SNPs) are linked with distal gene expression by spatial proximity, which helps to explain the causal role of disease-associated SNPs in non-coding region. Therefore, studies on spatial interactions between chromatin have created a new avenue for elucidating the mechanism of transcriptional regulation in disease pathogenesis. Recently, a growing number of ch...
6 Citations Source Cite
Published on May 1, 2015in Protein & Cell 6.23
Puping Liang7
Estimated H-index: 7
(SYSU: Sun Yat-sen University),
Yanwen Xu14
Estimated H-index: 14
(SYSU: Sun Yat-sen University)
+ 13 AuthorsYujing Li10
Estimated H-index: 10
(SYSU: Sun Yat-sen University)
Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious knowledge gap remains in our understanding of DNA repair mechanisms in human early embryos, and in the efficiency and potential off-target effects of using technologies such as CRISP...
458 Citations Source Cite
Published on Mar 18, 2015in Database 3.98
Zhenhua Luo6
Estimated H-index: 6
,
Zhiming Dai6
Estimated H-index: 6
+ 4 AuthorsYuanyan Xiong6
Estimated H-index: 6
Interaction network surrounding telomeres has been intensively studied during the past two decades. However, no specific resource by integrating telomere interaction information data is currently available. To facilitate the understanding of the molecular interaction network by which telomeres are associated with biological process and diseases, we have developed TeloPIN (Telomeric Proteins Interaction Network) database (http://songyanglab.sysu.edu.cn/telopin/), a novel database that points to p...
6 Citations Source Cite
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