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Hisato Jingami
Kyoto University
80Publications
31H-index
4,733Citations
Publications 80
Newest
Metabotropic glutamate receptors (mGluRs) are involved in the regulation of many physiological and pathological processes in the central nervous system. The extracellular domain (ECD) of mGluR subtype 3 (mGluR3) was produced using the baculovirus expression system and purified from the culture medium. However, the recombinant protein showed heterogeneity in molecular weight on SDS–PAGE analysis. It was found that the unglycosylation of Asn414 significantly reduced the heterogeneity. Consequently...
2 CitationsSource
#1Tsuyoshi Waku (Osaka University)H-Index: 13
#2Takuma Shiraki (Osaka University)H-Index: 11
Last.Kosuke Morikawa (Osaka University)H-Index: 55
view all 8 authors...
83 CitationsSource
#1Tsuyoshi Waku (Osaka University)H-Index: 13
#2Takuma Shiraki (Osaka University)H-Index: 11
Last.Kosuke Morikawa (Osaka University)H-Index: 55
view all 8 authors...
Abstract Peroxisome proliferator-activated receptor (PPAR) γ is a nuclear receptor that regulates lipid homeostasis, and several fatty acid metabolites have been identified as PPARγ ligands. Here, we present four crystal structures of the PPARγ ligand binding domain (LBD) covalently bound to endogenous fatty acids via a unique cysteine, which is reportedly critical for receptor activation. The structure analyses of the LBD complexed with 15-deoxy-Δ 12,14 -prostaglandin J 2 (15d-PGJ 2 ) revealed ...
89 CitationsSource
#1Takuma SugiH-Index: 3
#2Kosuke MorikawaH-Index: 55
Last.Hisato JingamiH-Index: 31
view all 3 authors...
#1Rei NomuraH-Index: 1
#2Yoshikazu SuzukiH-Index: 10
Last.Hisato JingamiH-Index: 31
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Abstract The γ-aminobutyric acid, type B (GABAB) receptor is a heterodimeric receptor consisting of two complementary subunits, GABAB1 receptor (GBR1) and GABAB2 receptor (GBR2). GBR1 is responsible for GABA binding, whereas GBR2 is considered to perform a critical role in signal transduction toward downstream targets. Therefore, precise communication between GBR1 and GBR2 is thought to be essential for the proper signal transduction process. However, biochemical data describing the interaction ...
12 CitationsSource
Abstract Lipid-mediated regulatory mechanism of the C-terminal ligand binding to PDZ domains is not fully understood, despite their roles in subcellular organization. Here, we provide structural insights into the phosphatidylinositol 4,5-bisphosphate (PIP 2 ) recognition mode of a PDZ domain, as revealed from the crystal structure of the phosphate-bound PDZ domain. Two adjacent phosphate ions bind to the basic residues close to the amino terminus of the α2 helix in the Tamalin PDZ domain, reflec...
12 CitationsSource
Glutamate is the major excitatory neurotransmitter and its metabotropic glutamate receptor (mGluR) plays an important role in the central nervous system. The ligand-binding domain (LBD) of mGluR subtype 7 (mGluR7) was produced using the baculovirus expression system and purified from the culture medium. The purified protein was characterized by gel-filtration chromatography, SDS–PAGE and a ligand-binding assay. Crystals of mGluR7 LBD were grown at 293 K by the hanging-drop vapour-diffusion metho...
3 CitationsSource
#1Takuma SugiH-Index: 3
#2Takuji OyamaH-Index: 23
Last.Hisato JingamiH-Index: 31
view all 6 authors...
Source
#1Takuma SugiH-Index: 3
#2Takuji OyamaH-Index: 23
Last.Hisato JingamiH-Index: 31
view all 6 authors...
Source
#1Takuma SugiH-Index: 3
#2Takuji OyamaH-Index: 23
Last.Hisato JingamiH-Index: 31
view all 6 authors...
Source
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