Olivier Rosnet
French Institute of Health and Medical Research
Receptor tyrosine kinaseGeneMolecular biologyGeneticsBiology
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Publications 48
#1Ange Line Bruel (University of Burgundy)H-Index: 5
#2Brunella Franco (University of Naples Federico II)H-Index: 43
Last. Christel Thauvin-RobinetH-Index: 36
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Oral–facial–digital syndromes (OFDS) gather rare genetic disorders characterised by facial, oral and digital abnormalities associated with a wide range of additional features (polycystic kidney disease, cerebral malformations and several others) to delineate a growing list of OFDS subtypes. The most frequent, OFD type I, is caused by a heterozygous mutation in the OFD1 gene encoding a centrosomal protein. The wide clinical heterogeneity of OFDS suggests the involvement of other ciliary genes. Fo...
17 CitationsSource
Oral-facial-digital (OFD) syndromes are rare heterogeneous disorders characterized by the association of abnormalities of the face, the oral cavity and the extremities, some due to mutations in proteins of the transition zone of the primary cilia or the closely associated distal end of centrioles. These two structures are essential for the formation of functional cilia, and for signaling events during development. We report here causal compound heterozygous mutations of KIAA0753/OFIP in a patien...
14 CitationsSource
#1Véronique ChevrierH-Index: 12
#2Frédérique LemboH-Index: 11
Last. Olivier RosnetH-Index: 29
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Stable microtubules organized on the basis of a nine-fold rotational symmetry constitute the structural basis of centrioles and basal bodies. A number of proteins associate with these structures to drive the process of centrosomes and cilia formation and to participate in their function. Pericentriolar satellites are small round-shaped particles closely associated with microtubules with PCM1 as a major component. To date they have been described only in vertebrates where they participate in the ...
#1Régine RoubinH-Index: 16
#2Claire AcquavivaH-Index: 6
Last. Olivier RosnetH-Index: 29
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The generation of cellular microtubules is initiated at specific sites such as the centrosome and the Golgi apparatus that contain nucleation complexes rich in γ-tubulin. The microtubule growing plus-ends are stabilized by plus-end tracking proteins (+TIPs), mainly EB1 and associated proteins. Myomegalin was identified as a centrosome/Golgi protein associated with cyclic nucleotide phosphodiesterase. We show here that Myomegalin exists as several isoforms. We characterize two of them. One isofor...
36 CitationsSource
#1A Aubusson-Fleury (University of Paris-Sud)H-Index: 2
#2Michel Lemullois (University of Paris-Sud)H-Index: 5
Last. Janine Beisson (University of Paris-Sud)H-Index: 32
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Summary Within the FOP family of centrosomal proteins, the conserved FOR20 protein has been implicated in the control of primary cilium assembly in human cells. To ascertain its role in ciliogenesis, we have investigated the function of its ortholog, PtFOR20p, in the multiciliated unicellular organism Paramecium . Using combined functional and cytological analyses, we found that PtFOR20p specifically localises at basal bodies and is required to build the transition zone, a prerequisite to their ...
29 CitationsSource
#1Fatima SedjaïH-Index: 2
#2Claire AcquavivaH-Index: 6
Last. Olivier RosnetH-Index: 29
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Cilia and flagella are evolutionary conserved organelles that generate fluid movement and locomotion, and play roles in chemosensation, mechanosensation and intracellular signalling. In complex organisms, cilia are highly diversified, which allows them to perform various functions; however, they retain a 9+0 or 9+2 microtubules structure connected to a basal body. Here, we describe FOR20 (FOP-related protein of 20 kDa), a previously uncharacterized and highly conserved protein that is required f...
38 CitationsSource
#1Claire AcquavivaH-Index: 6
#2Véronique ChevrierH-Index: 12
Last. Olivier RosnetH-Index: 29
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FOP is a centrosomal protein originally discovered as a fusion partner of FGFR1 in patients with a rare stem cell myeloproliferative disorder. In DT40 chicken lymphocytes, we show that the normal FOP protein localizes at the centrosome throughout the cell cycle and preferentially accumulates at the distal end of the mother centriole. We used homologous recombination in DT40 cells to generate an inducible null mutant for FOP. Loss of FOP induces apoptosis in the G1 phase of the cell cycle with ac...
18 CitationsSource
Myeloproliferative disorders (MPD) are clonal proliferative diseases of the hematopoietic stem cell with expansion of one or several blood cell lineages. Many of the characterized molecular alterations found in MPD are mutations and translocations of tyrosine kinase genes. Translocations lead to
4 CitationsSource
Abstract The VpreB3 gene product was first characterized as an immunoglobulin (Ig) μ heavy chain-binding protein in mouse precursor B (pre-B) cells. Although its function is unknown, it has been proposed to participate in the assembly and transport of the pre-B cell receptor. We have identified a VpreB3 orthologous gene in chicken that is located close to the immunoglobulin light chain (LC) gene cluster and specifically expressed in the bursa of Fabricius. By overexpressing VpreB3 in the DT40 Ig...
16 CitationsSource
#1Marchetto SH-Index: 4
#2Emmanuel FournierH-Index: 29
Last. Olivier RosnetH-Index: 29
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The FLT3 receptor tyrosine kinase and its ligand, FL, regulate the development of hematopoietic stem cells and early B lymphoid progenitors. FL has a strong capacity to boost production of dendritic and natural killer cells in vivo, thereby providing a new and promising tool for anti-cancer immunotherapy. Intracellular FLT3 signaling involves tyrosine phosphorylation of several cytoplasmic proteins including SHC. We have found that upon FLT3 activation SHC phosphorylation occurs at tyrosine 239/...
68 CitationsSource