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Kevin Blackburn
North Carolina State University
18Publications
12H-index
914Citations
Publications 17
Newest
#1Elena R. Schroeter (NCSU: North Carolina State University)H-Index: 9
#2Kevin Blackburn (NCSU: North Carolina State University)H-Index: 12
Last.Mary H. Schweitzer (NCSU: North Carolina State University)H-Index: 25
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Humic substances are breakdown products of decaying organic matter that co-extract with proteins from fossils. These substances are difficult to separate from proteins in solution and interfere wit...
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#1Elena R. SchroeterH-Index: 9
#2Kevin BlackburnH-Index: 12
Last.Mary H. SchweitzerH-Index: 25
view all 4 authors...
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#1Kevin Blackburn (NCSU: North Carolina State University)H-Index: 12
#2Ximena Bustamante-Marín (UNC: University of North Carolina at Chapel Hill)H-Index: 7
Last.Lawrence E. OstrowskiH-Index: 25
view all 5 authors...
Cilia are essential to many diverse cellular processes. Although many major axonemal components have been identified and studied, how they interact to form a functional axoneme is not completely understood. To further our understanding of the protein composition of human airway cilia, we performed a semiquantitative analysis of ciliary axonemes using label-free LC/MSE, which identified over 400 proteins with high confidence. Tubulins were the most abundant proteins identified, with evidence of 2...
18 CitationsSource
#1Laura E. Herring (NCSU: North Carolina State University)H-Index: 2
#2Kyle G. Grant (UNC: University of North Carolina at Chapel Hill)H-Index: 3
Last.Michael B. Goshe (NCSU: North Carolina State University)H-Index: 25
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Abstract Phosphorylation is an important post-translational modification that is involved in regulating many signaling pathways. Of particular interest are the growth factor mediated Ras and phosphoinositide 3-kinase (PI3K) signaling pathways which, if misregulated, can contribute to the progression of cancer. Phosphoproteomic methods have been developed to study regulation of signaling pathways; however, due to the low stoichiometry of phosphorylation, understanding these pathways is still a ch...
5 CitationsSource
#1S.H. MacKenzieH-Index: 7
#2J.L. SchipperH-Index: 8
Last.A. Clay ClarkH-Index: 17
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The conformational ensemble of procaspase 3, the primary executioner in apoptosis, contains two major forms, inactive and active, with the inactive state favored in the native ensemble. A region of the protein known as the intersubunit linker (IL) is cleaved during maturation, resulting in movement of the IL out of the dimer interface and subsequent active site formation (activation-by-cleavage mechanism). We examined two models for the role of the IL in maintaining the inactive conformer, an IL...
10 CitationsSource
#1Ko-yi ChienH-Index: 3
#2Kevin BlackburnH-Index: 12
Last.Michael B. GosheH-Index: 25
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Vaccination is an effective strategy to reduce the loss of chickens in the poultry industry caused by Marek’s Disease (MD), an avian lymphoproliferative disease. The vaccines currently used are from attenuated serotype 1 Marek’s disease virus (MDV) or naturally nononcogenic MDV strains. To prepare for future immunity breaks, functional genomic and proteomic studies have been used to better understand the underlying mechanisms of MDV pathogenicity and the effects induced by the vaccine viruses. I...
12 CitationsSource
#1Ricardo Vancini (NCSU: North Carolina State University)H-Index: 6
#2Angel Paredes (University of Texas Health Science Center at Houston)H-Index: 14
Last.Dennis Brown (NCSU: North Carolina State University)H-Index: 86
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Espirito Santo virus (ESV) is a newly discovered virus recovered as contamination in a sample of a virulent strain of dengue-2 virus (strain 44/2), which was recovered from a patient in the state of Espirito Santo, Brazil, and amplified in insect cells. ESV was found to be dependent upon coinfection with a virulent strain of dengue-2 virus and to replicate in C6/36 insect cells but not in mammalian Vero cells. A sequence of the genome has been produced by de novo assembly and was not found to ma...
12 CitationsSource
May 1, 2011 in ATS (American Thoracic Society International Conference)
#1Lawrence E. Ostrowski (UNC: University of North Carolina at Chapel Hill)H-Index: 25
Last.Michael B. Goshe (NCSU: North Carolina State University)H-Index: 25
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1 CitationsSource
#1Kevin BlackburnH-Index: 12
#2Flaubert MbeunkuiH-Index: 11
Last.Michael B. GosheH-Index: 25
view all 5 authors...
Performance differences in protein and proteome characterization achieved by data-independent acquisition (DIA) LC/MSE and data-dependent acquisition (DDA) LC/MS/MS approaches were investigated. LC/MSE is a novel mode of generating product ion data for all coeluting precursors in parallel as opposed to LC/MS/MS where coeluting precursors must be serially fragmented one at a time. During LC/MSE analysis, alternating MS scans of “normal” and “elevated” collision energy are collected at regular int...
62 CitationsSource
#1Kevin Blackburn (NCSU: North Carolina State University)H-Index: 12
#2Fang-yi Cheng (NCSU: North Carolina State University)H-Index: 4
Last.Michael B. Goshe (NCSU: North Carolina State University)H-Index: 25
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Plant cells secrete a wide variety of defense-related proteins into the extracellular space or apoplast in response to pathogen attack. One of these, mannitol dehydrogenase (MTD), is normally a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. Recent immunological and biochemical evidence, however, suggests that MTD is also secreted into the apoplast in response to pathogen attack, despite lacking a known peptide signal seque...
22 CitationsSource
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