Noriyuki Kishi
Keio University
Notch signaling pathwayNeuroscienceMarmosetRett syndromeBiology
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Publications 21
#1Wakako Kumita (Central Institute for Experimental Animals)H-Index: 2
#2Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
Last. Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 13 authors...
Genetically modified nonhuman primates (NHP) are useful models for biomedical research. Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been extensively investigated in marmosets. In this study, optimum conditions for target gene modification efficacies of CRISPR/mRNA and CRISPR/nuclease in marmoset embryos were examined. CRISPR/nuclease was more effective than CRISPR/mRNA in avoiding mosai...
1 CitationsSource
#1Sho Yoshimatsu (Keio: Keio University)H-Index: 2
#2Junko Okahara (Central Institute for Experimental Animals)H-Index: 5
Last. Hideyuki Okano (Keio: Keio University)H-Index: 112
view all 9 authors...
Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRI...
4 CitationsSource
#1Yawara Haga (Tokyo Metropolitan University)H-Index: 1
#2Jun-ichi Hata (Central Institute for Experimental Animals)H-Index: 43
Last. Akira Furukawa (Tokyo Metropolitan University)H-Index: 6
view all 11 authors...
1 CitationsSource
#1Hideyuki Okano (Keio: Keio University)H-Index: 112
#2Noriyuki Kishi (Keio: Keio University)H-Index: 11
Although mice have been the most frequently used experimental animals in many research fields due to well-established gene manipulation techniques, recent evidence has revealed that rodent models do not always recapitulate pathophysiology of human neurological and psychiatric diseases due to the differences between humans and rodents. The recent developments in gene manipulation of non-human primate have been attracting much attention in the biomedical research field, because non-human primates ...
12 CitationsSource
#1Tomomi Shimogori (Riken-BSI: RIKEN Brain Science Institute)H-Index: 26
#2Ayumi Abe (Riken-BSI: RIKEN Brain Science Institute)H-Index: 1
Last. Hideyuki Okano (Keio: Keio University)H-Index: 112
view all 29 authors...
Abstract Interest in the common marmoset ( Callithrix jacchus ) as a primate model animal has grown recently, in part due to the successful demonstration of transgenic marmosets. However, there is some debate as to the suitability of marmosets, compared to more widely used animal models, such as the macaque monkey and mouse. Especially, the usage of marmoset for animal models of human cognition and mental disorders, is still yet to be fully explored. To examine the prospects of the marmoset mode...
4 CitationsSource
#1Noriyuki Kishi (Riken-BSI: RIKEN Brain Science Institute)H-Index: 11
#2Hideyuki Okano (Riken-BSI: RIKEN Brain Science Institute)H-Index: 112
The common marmoset (Callithrix jacchus) is a small New World non-human primate indigenous to northeastern Brazil. This species has been attracting the attention of biomedical researchers and neuroscientists for its ease of handling and colony maintenance, unique behavioral characteristics, and several human-like traits, such as enriched social vocal communication and strong relationships between parents and offspring. Its high reproductive efficiency makes it particularly amenable for use in th...
#1Noriyuki Kishi (Harvard University)H-Index: 11
#2Jessica L. MacDonald (SU: Syracuse University)H-Index: 9
Last. Jeffrey D. Macklis (Harvard University)H-Index: 48
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Mutations in the transcriptional regulator Mecp2 cause the severe X-linked neurodevelopmental disorder Rett syndrome (RTT). In this study, we investigate genes that function downstream of MeCP2 in cerebral cortex circuitry, and identify upregulation of Irak1, a central component of the NF-κB pathway. We show that overexpression of Irak1 mimics the reduced dendritic complexity of Mecp2-null cortical callosal projection neurons (CPN), and that NF-κB signalling is upregulated in the cortex with Mec...
20 CitationsSource
#2Yohei OkadaH-Index: 30
Last. Hideyuki OkanoH-Index: 112
view all 15 authors...
#1Yuko Numasawa-Kuroiwa (Keio: Keio University)H-Index: 2
#2Yohei Okada (Aichi Medical University)H-Index: 30
Last. Hideyuki Okano (Keio: Keio University)H-Index: 112
view all 15 authors...
Pelizaeus-Merzbacher disease (PMD) is a form of X-linked leukodystrophy caused by mutations in the proteolipid protein 1 (PLP1) gene. Although PLP1 proteins with missense mutations have been shown to accumulate in the rough endoplasmic reticulum (ER) in disease model animals and cell lines transfected with mutant PLP1 genes, the exact pathogenetic mechanism of PMD has not previously been clarified. In this study, we established induced pluripotent stem cells (iPSCs) from two PMD patients carryin...
60 CitationsSource
#1Noriyuki Kishi (Keio: Keio University)H-Index: 11
#2Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
Last. Hideyuki Okano (Keio: Keio University)H-Index: 112
view all 4 authors...
The common marmoset (Callithrix jacchus) is a small New World primate; it originally comes from the Atlantic coastal forests in northeastern Brazil. It has been attracting much attention in the biomedical research field because of its size, availability, and unique biological characteristics. Its endocrinological and behavioral similarity to humans, comparative ease in handling, and high reproductive efficiency are very advantageous for neuroscience research. Recently, we developed transgenic co...
99 CitationsSource