Tomoki Takashina
Induced pluripotent stem cellCell-penetrating peptideArtificial transcription factorReprogrammingCell biology
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Publications 6
Immune checkpoint blockade (ICB) therapy improved the prognosis of cancer patients, but general administration of ICBs occasionally induces side effects that include immune-related adverse events and tumour hyper-progression. Here, we established a protein-based system, by which endogenous expression of IC molecule in natural killer (NK) cells was transiently repressed on enhancement of their antitumour activity. A protein-based genome modulator (GM) system is composed of a transcription activat...
#1Tomoki TakashinaH-Index: 1
#2Takayoshi KoyamaH-Index: 6
Last. Yukihito IshizakaH-Index: 30
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Abstract Cellular reprogramming is a promising technology in regenerative medicine, but most studies have been performed by using expression vectors. For future clinical applications, it is necessary to establish a system in which cell engineering can be manipulated without any risk of damaging the genome. Here, we identified a cell-penetrating peptide composed of 10 amino acids (RIFIHFRIGC) with nuclear trafficking activity and found that it was significantly more potent than a Tat-derived pept...
3 CitationsSource
#1Tomoki TakashinaH-Index: 1
#2Yukihito IshizakaH-Index: 30
Direct reprogramming is a promising technology in regenerative medicine. However, there is no report on the clinical applications of cells prepared by direct reprogramming. In the current review, we describe direct reprogramming methods of somatic cells to hepatocytes, pancreatic ?-cells, cardiomyocytes, and endothelial cells. Next, we discuss current issues that should be clarified for their future clinical applications. As the most critical issue, it is necessary to establish a vector-free sys...
#1Yukihito IshizakaH-Index: 30
#2Tomoki TakashinaH-Index: 1