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M.Teresa Baquero
University of Florida
2Publications
2H-index
44Citations
Publications 2
Newest
#1Lauren M. Brum (UF: University of Florida)H-Index: 2
#2Peter C. Turner (UF: University of Florida)H-Index: 24
Last.Richard W. Moyer (UF: University of Florida)H-Index: 32
view all 5 authors...
Abstract The cowpox virus (CPV) glycoprotein serpin SPI-3, a functional protease inhibitor, and the viral hemagglutinin (HA) are required to prevent fusion of wt CPV infected cells. SPI-3 and HA from CPV infected cells co-localize to the plasma membrane and are found in extracellular enveloped virus (EEV). We also show that an N-terminal SPI-3 signal sequence, but not glycosylation, is required for membrane localization and fusion inhibition. In the absence of HA (CPVΔHA), no SPI-3 is found on t...
22 CitationsSource
#1Peter C. Turner (UF: University of Florida)H-Index: 24
#2M.Teresa Baquero (UF: University of Florida)H-Index: 2
Last.Richard W. Moyer (UF: University of Florida)H-Index: 32
view all 5 authors...
Abstract The orthopoxvirus serpin SPI-3 is N-glycosylated and suppresses fusion between infected cells. Although SPI-3 contains motifs conserved in inhibitory serpins, no proteinase inhibition by SPI-3 has been demonstrated, and mutations within the serpin reactive center loop (RCL) do not affect the ability to regulate cell fusion. We demonstrate here that SPI-3 protein expressed by transcription/translation in vitro is able to form SDS-stable complexes with the serine proteinases plasmin, urok...
22 CitationsSource
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