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Koray Dogan Kaya
National Institutes of Health
9Publications
6H-index
151Citations
Publications 9
Newest
Published in bioRxiv
Koray Dogan Kaya6
Estimated H-index: 6
(NIH: National Institutes of Health),
Hsiu-Chuan Chen2
Estimated H-index: 2
(NIH: National Institutes of Health)
+ -3 AuthorsK. Kruczek (NIH: National Institutes of Health)
Retinal organoids generated from human pluripotent stem cells exhibit considerable variability in temporal dynamics of differentiation. To assess the maturity of neural retina in vitro, we performed transcriptome analyses of developing organoids from human embryonic and induced pluripotent stem cell lines. We show that the developmental variability in organoids was reflected in gene expression profiles and could be evaluated by molecular staging with the human fetal and adult retinal transcripto...
Published on Oct 15, 2018in Human Molecular Genetics4.54
Juthaporn Assawachananont2
Estimated H-index: 2
(NIH: National Institutes of Health),
Soo-Young Kim3
Estimated H-index: 3
(NIH: National Institutes of Health)
+ 3 AuthorsAnand Swaroop72
Estimated H-index: 72
(NIH: National Institutes of Health)
Published on Jul 13, 2018in Investigative Ophthalmology & Visual Science3.81
Holly Ho Chen3
Estimated H-index: 3
(NIH: National Institutes of Health),
Koray Dogan Kaya6
Estimated H-index: 6
(NIH: National Institutes of Health)
+ 6 AuthorsAnand Swaroop72
Estimated H-index: 72
(NIH: National Institutes of Health)
Published on Jan 1, 2018in Stem cell reports5.50
Tyler DiStefano2
Estimated H-index: 2
(NIH: National Institutes of Health),
Holly Yu Chen2
Estimated H-index: 2
(NIH: National Institutes of Health)
+ 6 AuthorsAnand Swaroop72
Estimated H-index: 72
(NIH: National Institutes of Health)
Summary Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a polarized, laminated architecture. In static cultures, organoid development may be hindered by limitations in diffusion of oxygen and nutrients. Herein, we report a bioprocess using rotating-wall vessel (RWV) bioreactors to culture retinal organoids derived from mouse pluripotent stem cells. Organoids in RWV demonstrate enhanced proliferation, with well-defined morphology ...
Published on Nov 1, 2016in Cell Reports7.82
Jung-Woong Kim12
Estimated H-index: 12
(CAU: Chung-Ang University),
Hyun-Jin Yang6
Estimated H-index: 6
(NIH: National Institutes of Health)
+ 15 AuthorsDustin Thad Whitaker1
Estimated H-index: 1
(A&M: Texas A&M University)
Summary Gene regulatory networks (GRNs) guiding differentiation of cell types and cell assemblies in the nervous system are poorly understood because of inherent complexities and interdependence of signaling pathways. Here, we report transcriptome dynamics of differentiating rod photoreceptors in the mammalian retina. Given that the transcription factor NRL determines rod cell fate, we performed expression profiling of developing NRL-positive (rods) and NRL-negative (S-cone-like) mouse photorece...
Published on Oct 1, 2016in Cell Reports7.82
Helen May-Simera19
Estimated H-index: 19
(NIH: National Institutes of Health),
Jessica Gumerson2
Estimated H-index: 2
(NIH: National Institutes of Health)
+ 11 AuthorsFriedrich Kretschmer8
Estimated H-index: 8
(NIH: National Institutes of Health)
Summary Microtubule actin crosslinking factor 1 (MACF1) plays a role in the coordination of microtubules and actin in multiple cellular processes. Here, we show that MACF1 is also critical for ciliogenesis in multiple cell types. Ablation of Macf1 in the developing retina abolishes ciliogenesis, and basal bodies fail to dock to ciliary vesicles or migrate apically. Photoreceptor polarity is randomized, while inner retinal cells laminate correctly, suggesting that photoreceptor maturation is guid...
Published on Sep 9, 2016in Molecular Vision2.17
Holly Yu Chen2
Estimated H-index: 2
,
Koray Dogan Kaya6
Estimated H-index: 6
+ 1 AuthorsAnand Swaroop72
Estimated H-index: 72
Purpose The generation of three-dimensional (3D) organoids with optic cup–like structures from pluripotent stem cells has created opportunities for investigating mammalian retinal development in vitro. However, retinal organoids in culture do not completely reflect the developmental state and in vivo architecture of the rod-dominant mouse retina. The goals of this study were to develop an efficient protocol for generating retinal organoids from stem cells and examine the morphogenesis of rods in...
Published on Apr 26, 2016in Investigative Ophthalmology & Visual Science3.81
Rossukon Kaewkhaw2
Estimated H-index: 2
(MU: Mahidol University),
Manju Swaroop8
Estimated H-index: 8
(NIH: National Institutes of Health)
+ 11 AuthorsJizhong Zou6
Estimated H-index: 6
(International Practical Shooting Confederation)
We discuss the use of pluripotent stem cell lines carrying fluorescent reporters driven by retinal promoters to derive three-dimensional (3-D) retina in culture and how this system can be exploited for elucidating human retinal biology, creating disease models in a dish, and designing targeted drug screens for retinal and macular degeneration. Furthermore, we realize that stem cell investigations are labor-intensive and require extensive resources. To expedite scientific discovery by sharing of ...
Published on Dec 1, 2015in Stem Cells5.61
Rossukon Kaewkhaw2
Estimated H-index: 2
(MU: Mahidol University),
Koray Dogan Kaya6
Estimated H-index: 6
(NIH: National Institutes of Health)
+ 5 AuthorsAnand Swaroop72
Estimated H-index: 72
(NIH: National Institutes of Health)
The derivation of three-dimensional (3D) stratified neural retina from pluripotent stem cells has permitted investigations of human photoreceptors. We have generated a H9 human embryonic stem cell subclone that carries a green fluorescent protein (GFP) reporter under the control of the promoter of cone-rod homeobox (CRX), an established marker of postmitotic photoreceptor precursors. The CRXp-GFP reporter replicates endogenous CRX expression in vitro when the H9 subclone is induced to form self-...
Published on Jun 11, 2015in Investigative Ophthalmology & Visual Science3.81
Koray Dogan Kaya6
Estimated H-index: 6
(NIH: National Institutes of Health),
Matthew Brooks22
Estimated H-index: 22
(NIH: National Institutes of Health)
+ 3 AuthorsAnand Swaroop72
Estimated H-index: 72
(NIH: National Institutes of Health)
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