Match!
Koray Dogan Kaya
National Institutes of Health
12Publications
7H-index
227Citations
Publications 13
Newest
#1Koray Dogan KayaH-Index: 7
#2Holly Yu ChenH-Index: 3
Last.Anand SwaroopH-Index: 75
view all 16 authors...
Purpose: Retinal organoids generated from human pluripotent stem cells exhibit considerable variability during differentiation. Our goals are to assess developmental maturity of the neural retina in vitro and design improved protocols based on objective criteria. Methods: We performed transcriptome analyses of developing retinal organoids from human embryonic and induced pluripotent stem cell lines and utilized multiple bioinformatic tools for comparative analysis. Immunohistochemistry, immunobl...
1 Citations
#1Koray Dogan Kaya (NIH: National Institutes of Health)H-Index: 7
#2Holly Yu Chen (NIH: National Institutes of Health)H-Index: 3
Last.Anand Swaroop (NIH: National Institutes of Health)H-Index: 75
view all 16 authors...
Retinal organoids generated from human pluripotent stem cells exhibit considerable variability in temporal dynamics of differentiation. To assess the maturity of neural retina in vitro, we performed transcriptome analyses of developing organoids from human embryonic and induced pluripotent stem cell lines. We show that the developmental variability in organoids was reflected in gene expression profiles and could be evaluated by molecular staging with the human fetal and adult retinal transcripto...
1 CitationsSource
#1Juthaporn Assawachananont (NIH: National Institutes of Health)H-Index: 2
#2Soo-Young Kim (NIH: National Institutes of Health)H-Index: 5
Last.Anand Swaroop (NIH: National Institutes of Health)H-Index: 75
view all 6 authors...
In the mammalian retina, rod and cone photoreceptors transmit the visual information to bipolar neurons through highly specialized ribbon synapses. We have limited understanding of regulatory pathways that guide morphogenesis and organization of photoreceptor presynaptic architecture in the developing retina. While neural retina leucine zipper (NRL) transcription factor determines rod cell fate and function, cone-rod homeobox (CRX) controls the expression of both rod- and cone-specific genes and...
3 CitationsSource
#1Holly Yu Chen (NIH: National Institutes of Health)H-Index: 3
#2Koray Dogan Kaya (NIH: National Institutes of Health)H-Index: 7
Last.Anand Swaroop (NIH: National Institutes of Health)H-Index: 75
view all 9 authors...
#1Tyler DiStefano (NIH: National Institutes of Health)H-Index: 2
#2Holly Yu Chen (NIH: National Institutes of Health)H-Index: 3
Last.Anand Swaroop (NIH: National Institutes of Health)H-Index: 75
view all 9 authors...
Summary Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a polarized, laminated architecture. In static cultures, organoid development may be hindered by limitations in diffusion of oxygen and nutrients. Herein, we report a bioprocess using rotating-wall vessel (RWV) bioreactors to culture retinal organoids derived from mouse pluripotent stem cells. Organoids in RWV demonstrate enhanced proliferation, with well-defined morphology ...
28 CitationsSource
#1Jung-Woong Kim (CAU: Chung-Ang University)H-Index: 12
#2Hyun-Jin Yang (NIH: National Institutes of Health)H-Index: 6
Last.Anand Swaroop (NIH: National Institutes of Health)H-Index: 75
view all 18 authors...
Summary Gene regulatory networks (GRNs) guiding differentiation of cell types and cell assemblies in the nervous system are poorly understood because of inherent complexities and interdependence of signaling pathways. Here, we report transcriptome dynamics of differentiating rod photoreceptors in the mammalian retina. Given that the transcription factor NRL determines rod cell fate, we performed expression profiling of developing NRL-positive (rods) and NRL-negative (S-cone-like) mouse photorece...
38 CitationsSource
#1Helen May-Simera (NIH: National Institutes of Health)H-Index: 20
#2Jessica Gumerson (NIH: National Institutes of Health)H-Index: 2
Last.Tiansen Li (NIH: National Institutes of Health)H-Index: 14
view all 14 authors...
Summary Microtubule actin crosslinking factor 1 (MACF1) plays a role in the coordination of microtubules and actin in multiple cellular processes. Here, we show that MACF1 is also critical for ciliogenesis in multiple cell types. Ablation of Macf1 in the developing retina abolishes ciliogenesis, and basal bodies fail to dock to ciliary vesicles or migrate apically. Photoreceptor polarity is randomized, while inner retinal cells laminate correctly, suggesting that photoreceptor maturation is guid...
15 CitationsSource
#1Holly Yu ChenH-Index: 3
#2Koray Dogan KayaH-Index: 7
Last.Anand SwaroopH-Index: 75
view all 4 authors...
Purpose The generation of three-dimensional (3D) organoids with optic cup–like structures from pluripotent stem cells has created opportunities for investigating mammalian retinal development in vitro. However, retinal organoids in culture do not completely reflect the developmental state and in vivo architecture of the rod-dominant mouse retina. The goals of this study were to develop an efficient protocol for generating retinal organoids from stem cells and examine the morphogenesis of rods in...
29 Citations
#1Rossukon Kaewkhaw (MU: Mahidol University)H-Index: 5
#2Manju Swaroop (NIH: National Institutes of Health)H-Index: 18
Last.Anand Swaroop (NIH: National Institutes of Health)H-Index: 75
view all 14 authors...
We discuss the use of pluripotent stem cell lines carrying fluorescent reporters driven by retinal promoters to derive three-dimensional (3-D) retina in culture and how this system can be exploited for elucidating human retinal biology, creating disease models in a dish, and designing targeted drug screens for retinal and macular degeneration. Furthermore, we realize that stem cell investigations are labor-intensive and require extensive resources. To expedite scientific discovery by sharing of ...
19 CitationsSource
#1Rossukon Kaewkhaw (MU: Mahidol University)H-Index: 5
#2Koray Dogan Kaya (NIH: National Institutes of Health)H-Index: 7
Last.Anand Swaroop (NIH: National Institutes of Health)H-Index: 75
view all 8 authors...
The derivation of three-dimensional (3D) stratified neural retina from pluripotent stem cells has permitted investigations of human photoreceptors. We have generated a H9 human embryonic stem cell subclone that carries a green fluorescent protein (GFP) reporter under the control of the promoter of cone-rod homeobox (CRX), an established marker of postmitotic photoreceptor precursors. The CRXp-GFP reporter replicates endogenous CRX expression in vitro when the H9 subclone is induced to form self-...
61 CitationsSource
12