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Yongchang Chen
Kunming University of Science and Technology
24Publications
13H-index
1,239Citations
Publications 23
Newest
#1Chu Chu (Kunming University of Science and Technology)H-Index: 1
#2Zhaohui Yang (Kunming University of Science and Technology)H-Index: 17
Last.Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
view all 10 authors...
Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonstrated that ...
Source
#1Bo Zhang (Kunming University of Science and Technology)
#2Zhigang Zhou (Kunming University of Science and Technology)H-Index: 1
Last.Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
view all 8 authors...
Source
#1Lei Shi (KIZ: Kunming Institute of Zoology)H-Index: 10
#2Xin LuoH-Index: 2
Last.Bing Su (KIZ: Kunming Institute of Zoology)H-Index: 23
view all 20 authors...
Brain size and cognitive skills are the most dramatically changed traits in humans during evolution and yet the genetic mechanisms underlying these human-specific changes remain elusive. Here, we successfully generated 11 transgenic rhesus monkeys (8 first-generation and 3 second-generation) carrying human copies of MCPH1, an important gene for brain development and brain evolution. Brain-image and tissue-section analyses indicated an altered pattern of neural-cell differentiation, resulting in ...
5 CitationsSource
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Puhao Xiao (Kunming University of Science and Technology)H-Index: 1
Last.Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 15 authors...
The CRISPR-mediated Cas system is the most widely used tool in gene editing and gene therapy for its convenience and efficiency. Delivery of the CRISPR system by adeno-associated viruses (AAVs) is currently the most promising approach to gene therapy. However, pre-existing adaptive immune responses against CRISPR nuclease (PAIR-C) and AAVs has been found in human serum, indicating that immune response is a problem that cannot be ignored, especially for in vivo gene correction. Non-human primates...
1 CitationsSource
#1Dongdong Qin (Kunming University of Science and Technology)H-Index: 1
#2Shihao Wu (KIZ: Kunming Institute of Zoology)H-Index: 1
Last.Xintian Hu (KIZ: Kunming Institute of Zoology)H-Index: 16
view all 4 authors...
Abstract Autism is a behaviorally defined neurodevelopmental disease characterized by social communication and social interaction deficits, as well as repetitive behaviors and restricted interests. Although the causes of autism are extremely complex, involving genes, environments and gene-environment interactions, genetic mutations are the important risk factors. For this reason, preclinical research is now shifting into focusing on generating transgenic and knockout models, especially using mac...
1 CitationsSource
#1Shuang Wang (Kunming University of Science and Technology)H-Index: 2
#2Shuaiwei Ren (Kunming University of Science and Technology)H-Index: 1
Last.Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
view all 10 authors...
8 CitationsSource
#1Shuang Wang (Kunming University of Science and Technology)H-Index: 2
#2Lichuan Yang (KIZ: Kunming Institute of Zoology)
Last.Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
view all 8 authors...
ABSTRACTThe tumor suppressor p53 is a key regulator of cell apoptosis and cell cycle arrest. Recent studies show that the delicate balance of p53 expression is important for neural tube defects, ne...
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#1Yiqiang Cui (Nanjing Medical University)H-Index: 11
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 15 authors...
Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination
13 CitationsSource
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Juehua Yu (Tongji University)H-Index: 15
Last.Yi Eve Sun (Tongji University)H-Index: 4
view all 37 authors...
Gene-editing technologies have made it feasible to create nonhuman primate models for human genetic disorders. Here, we report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys serving as a model for a neurodevelopmental disorder, Rett syndrome (RTT), which is caused by loss-of-function mutations in the human MECP2 gene. Male mutant monkeys were embryonic lethal, reiterating that RTT is a disease of females. Through a battery of behavioral analyses, including prim...
48 CitationsSource
#1Fei GaoH-Index: 13
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Tao Tan (Kunming University of Science and Technology)H-Index: 10
view all 16 authors...
Abstract Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylatio...
15 CitationsSource
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