Saravanabhavan Thangavel
Saint Louis University
Origin recognition complexMolecular biologyReplication factor CReplication fork reversalBiology
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Publications 5
#1Bo-Ruei Chen (Cornell University)H-Index: 4
#2Annabel Quinet (SLU: Saint Louis University)H-Index: 11
Last. Barry P. Sleckman (Cornell University)H-Index: 46
view all 12 authors...
XRCC4-like factor (XLF) is a non-homologous end joining (NHEJ) DNA double strand break repair protein. However, XLF deficiency leads to phenotypes in mice and humans that are not necessarily consistent with an isolated defect in NHEJ. Here we show that XLF functions during DNA replication. XLF undergoes cell division cycle 7–dependent phosphorylation; associates with the replication factor C complex, a critical component of the replisome; and is found at replication forks. XLF deficiency leads t...
1 CitationsSource
#1Saravanabhavan Thangavel (SLU: Saint Louis University)H-Index: 4
#2Matteo Berti (SLU: Saint Louis University)H-Index: 9
Last. Alessandro Vindigni (SLU: Saint Louis University)H-Index: 19
view all 14 authors...
Accurate processing of stalled or damaged DNA replication forks is paramount to genomic integrity and recent work points to replication fork reversal and restart as a central mechanism to ensuring high-fidelity DNA replication. Here, we identify a novel DNA2- and WRN-dependent mechanism of reversed replication fork processing and restart after prolonged genotoxic stress. The human DNA2 nuclease and WRN ATPase activities functionally interact to degrade reversed replication forks with a 5′-to-3′ ...
134 CitationsSource
#1Xiaoling Li (NIH: National Institutes of Health)H-Index: 27
#2Xing Lu (HU: Howard University)H-Index: 3
Last. Sudha Sharma (HU: Howard University)H-Index: 24
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The RECQ protein family of helicases has critical roles in protecting and stabilizing the genome. Three of the 5 known members of the human RecQ family are genetically linked with cancer susceptibility syndromes, but the association of the most abundant human RecQ homolog, RECQ1, with cellular transformation is yet unclear. RECQ1 is overexpressed in a variety of human cancers, indicating oncogenic functions. Here, we assessed genome-wide changes in gene expression upon knockdown of RECQ1 in HeLa...
19 CitationsSource
#1Matteo Berti (SLU: Saint Louis University)H-Index: 9
#2Arnab Ray Chaudhuri (UZH: University of Zurich)H-Index: 10
Last. Alessandro Vindigni (SLU: Saint Louis University)H-Index: 19
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Topoisomerase I (TOP1) inhibitors are an important class of anticancer drugs. The cytotoxicity of TOP1 inhibitors can be modulated by replication fork reversal through a process that requires poly(ADP-ribose) polymerase (PARP) activity. Whether regressed forks can efficiently restart and what factors are required to restart fork progression after fork reversal are still unknown. We have combined biochemical and EM approaches with single-molecule DNA fiber analysis to identify a key role for huma...
180 CitationsSource
#1Saravanabhavan Thangavel (Scuola Normale Superiore di Pisa)H-Index: 4
#2Ramiro Mendoza-Maldonado (International Centre for Genetic Engineering and Biotechnology)H-Index: 13
Last. Alessandro Vindigni (International Centre for Genetic Engineering and Biotechnology)H-Index: 22
view all 9 authors...
Cellular and biochemical studies support a role for all five human RecQ helicases in DNA replication; however, their specific functions during this process are unclear. Here we investigate the in vivo association of the five human RecQ helicases with three well-characterized human replication origins. We show that only RECQ1 (also called RECQL or RECQL1) and RECQ4 (also called RECQL4) associate with replication origins in a cell cycle-regulated fashion in unperturbed cells. RECQ4 is recruited to...
95 CitationsSource