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Fabien Brette
University of Leeds
22Publications
11H-index
855Citations
Publications 22
Newest
#1Bruno D. Stuyvers (St. John's University)H-Index: 3
#2Penelope A. Boyden (Columbia University)H-Index: 45
Last.Sebastien ChaigneH-Index: 1
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BACKGROUND. The trigger of ventricular tachyarrhythmias after myocardial infarction (MI) has been attributed to increased incidence of electrogenic Ca2+ waves in cardiac Purkinje cells (Pcells). A change in Ca2+ release channel (CRC) gating properties and overload of intracellular Ca2+ store are 2 potential causes of this increase. We analysed through a model of intracellular Ca2+ dynamics the spontaneous Ca2+ transients of Pcells after MI. Regional protein expression was assessed for potential ...
#1Caroline CrosH-Index: 5
#2Laurent SalléH-Index: 5
Last.Fabien BretteH-Index: 11
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Cardiac contraction is mediated by a transient increase in calcium ([Ca]) inside the cytoplasm. In mammalian cardiomyocytes most of the calcium for cardiac contraction is from the calcium stored in the sarcoplasmic reticulum (SR). In fish cardiomyocytes, the relative contribution of calcium coming from the extracellular space versus SR is not clear. This study addresses this point. Trout ventricular myocytes were enzymatically isolated. Calcium current (ICa) was recorded using the patch clamp te...
#1Fabien Brette (University of Leeds)H-Index: 11
#2J Leroy (UCL: University College London)H-Index: 7
Last.Laurent Sallé (UNICAEN: University of Caen Lower Normandy)H-Index: 11
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Abstract Calcium is a ubiquitous second messenger which plays key roles in numerous physiological functions. In cardiac myocytes, Ca 2+ crosses the plasma membrane via specialized voltage-gated Ca 2+ channels which have two main functions: (i) carrying depolarizing current by allowing positively charged Ca 2+ ions to move into the cell; (ii) triggering Ca 2+ release from the sarcoplasmic reticulum. Recently, it has been suggested than Ca 2+ channels also participate in excitation–transcription c...
#1Fabien BretteH-Index: 11
#2Laurent SalléH-Index: 5
Last.Clive H. OrchardH-Index: 34
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We have characterized modulation of I Ca by Ca 2+ at the t-tubules (ie, in control cells) and surface sarcolemma (ie, in detubulated cells) of cardiac ventricular myocytes, using the whole-cell patch clamp technique to record I Ca . I Ca inactivation was significantly slower in detubulated cells than in control cells (27.1±7.8 ms, n=22, versus 16.4±7.9 ms, n=22; P I Ca inactivation was not changed by the treatment used to produce detubulation. In the presence of ryanodine or BAPTA, or when Ba 2+...
#1Fabien Brette (University of Leeds)H-Index: 11
#2Patricia Rodriguez (University of Leeds)H-Index: 8
Last.Clive H. Orchard (University of Leeds)H-Index: 34
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Abstract β-adrenergic stimulation helps to synchronize Ca release in myocytes from failing hearts. Transverse (t-) tubules, which synchronize Ca release in normal cells and contain many of the elements of the β-adrenergic pathway, may be depleted in such cells. The objective of the present study was to determine whether β-adrenergic stimulation could reverse the desynchronization of Ca release observed in detubulated ventricular myocytes. The effect of isoprenaline (0.5 μM) on control and detubu...
The transverse tubules (t-tubules) of mammalian cardiac ventricular myocytes are invaginations of the surface membrane. Recent studies have suggested that the structure and function of the t-tubules are more complex than previously believed; in particular, many of the proteins involved in cellular Ca2+ cycling appear to be concentrated at the t-tubule. Thus, the t-tubules are an important determinant of cardiac cell function, especially as the main site of excitation-contraction coupling, ensuri...
The transverse tubules (t-tubules) of mammalian cardiac ventricular myocytes are invaginations of the surface membrane. Recent studies have suggested that the structure and function of the t-tubules are more complex than previously believed; in particular, many of the proteins involved in cellular Ca2+ cycling appear to be concentrated at the t-tubule. Thus, the t-tubules are an important determinant of cardiac cell function, especially as the main site of excitation-contraction coupling, ensuri...
#1Fabien Brette (University of Leeds)H-Index: 11
#2Kimiaki Komukai (University of Leeds)H-Index: 7
Last.Clive H. Orchard (University of Leeds)H-Index: 34
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Kawai M, Hussain M, and Orchard CH.Am J Heart Circ Physiol 277: H603–H609, 1999 developed a technique to detubulate rat ventricular myocytes using formamide and showed that detubulation results in ...
#1Zhaokang Yang (University of Leeds)H-Index: 12
#2Caroline Pascarel (University of Leeds)H-Index: 3
Last.Clive H. Orchard (University of Leeds)H-Index: 34
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Detubulation of rat ventricular myocytes has been used to investigate the role of the t-tubules in Ca 2+ cycling during excitation-contraction coupling in rat ventricular myocytes. Ca 2+ was monitored using fluo-3 and confocal microscopy. In control myocytes, electrical stimulation caused a spatially uniform increase in intracellular [Ca 2+ ] across the cell width. After detubulation, [Ca 2+ ] rose initially at the cell periphery and then propagated into the center of the cell. Application of ca...
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