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Xiaolong Wang
Northwest A&F University
GeneGenome editingGeneticsBiologyCRISPR
42Publications
10H-index
375Citations
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Publications 49
Newest
#1Xiaolong Wang (NWAFU: Northwest A&F University)H-Index: 10
#2Yulin Chen (NWAFU: Northwest A&F University)H-Index: 15
Last. Kui LiH-Index: 1
view all 6 authors...
1 CitationsSource
#3Ke ZhangH-Index: 2
view all 9 authors...
Environmental adaptation of ruminants was highly related to microbiota in the rumen. To investigate the diversity and composition of bacteria, fungi, and protozoa in the rumen of high-altitude animals, amplicon gene sequencing was performed using rumen fluid samples derived from both Tibetan goats and sheep at the same pasture in a highland (altitude > 4800 m). Between these two species, the ruminal bacteria and fungi were significantly different at multiple taxonomic levels. The alpha diversity...
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#1Lei Wang (Qinghai University)
#2Ke Zhang (NWAFU: Northwest A&F University)H-Index: 2
Last. Guofang Wu (Qinghai University)
view all 7 authors...
The productivity of ruminants depends largely on rumen microbiota. However, there are few studies on the age-related succession of rumen microbial communities in grazing lambs. Here, we conducted 16 s rRNA gene sequencing for bacterial identification on rumen fluid samples from 27 Tibetan lambs at nine developmental stages (days (D) 0, 2, 7, 14, 28, 42, 56, 70, and 360, n = 3). We observed that Bacteroidetes and Proteobacteria populations were significantly changed during the growing lambs’ firs...
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#1Guanwei Li (NWAFU: Northwest A&F University)H-Index: 3
#2Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 6
Last. Xiaolong Wang (NWAFU: Northwest A&F University)H-Index: 10
view all 23 authors...
The ability to alter single bases without homology directed repair (HDR) of double-strand breaks provides a potential solution for editing livestock genomes for economic traits, which are often multigenic. Progress toward multiplex editing in large animals has been hampered by the costly inefficiencies of HDR via microinjection of in vitro manipulated embryos. Here, we designed sgRNAs to induce nonsense codons (C-to-T transitions) at four target sites in caprine FGF5, which is a crucial regulato...
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#1Ke ZhangH-Index: 2
#2Bibo LiH-Index: 2
view all 8 authors...
With increasing age, the rumen microbiota of new-born ruminants become central in the translation of fibrous feed substances into essential nutrients. However, the colonization process of the microbial community (especially fungal community) remains poorly understood in ruminants at pre-weaning stages. In this study, the rumen bacterial and fungal colonization processes were investigated in goats at eight stages using amplicon sequencing. For bacteria, we found 36 common core genera at D0, D3, D...
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#1Xufeng Han (NWAFU: Northwest A&F University)H-Index: 2
#2Bibo Li (NWAFU: Northwest A&F University)H-Index: 2
Last. Yuxin Yang (NWAFU: Northwest A&F University)H-Index: 9
view all 5 authors...
Abstract The rumen contains a highly complex microbial ecosystem that plays an important role in converting solar energy in plants into nutrients for ruminants and generates animal food products, such as meat and milk for humans. Therefore, understanding the effect of the dietary concentrate to forage (C:F) ratio on ruminal microbiota is of great significance for the growth and development of ruminants. In this study, changes in the ruminal bacterial and anaerobic fungal populations of Shaanbei ...
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#1Bibo Li (NWAFU: Northwest A&F University)H-Index: 2
#2Ke Zhang (NWAFU: Northwest A&F University)H-Index: 2
Last. Yuxin Yang (NWAFU: Northwest A&F University)H-Index: 9
view all 6 authors...
Bacterial communities in gastrointestinal tracts (GIT) play an important role in animal health and performance. Despite its importance, little information is available on the establishment of microbial populations in the goat GIT or on changes occurring during early development. Therefore, this study investigated the bacterial community dynamics of the rumen, duodenum, jejunum, ileum, cecum, and colon in 15 goats at five developmental stages (0, 14, 28, 42, and 56 days old) by using 16S rDNA seq...
1 CitationsSource
#1Peter Girgis Tawfek Kalds (NWAFU: Northwest A&F University)H-Index: 1
#2Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 6
Last. Yulin Chen (NWAFU: Northwest A&F University)H-Index: 15
view all 9 authors...
Sheep and goats are valuable livestock species that have been raised for their production of meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period, and abundant secretion of milk, sheep and goats have become important model animals in agricultural, pharmaceutical, and biomedical research. Genome engineering has been widely applied to sheep and goat research. Pronuclear injection and somatic cell nuclear transfer represent the two primary procedures for the ...
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#1Lamei Wang (NWAFU: Northwest A&F University)H-Index: 1
#2Sen Ma (NWAFU: Northwest A&F University)H-Index: 3
Last. Yulin Chen (NWAFU: Northwest A&F University)H-Index: 15
view all 5 authors...
Background: Myostatin (MSTN) negatively regulates muscle mass and is a potent regulator of energy metabolism. However, MSTN knockout have affect mitochondrial function. This research assessed the mitochondrial energy metabolism of Mstn-/+ KO cells, and wondered whether the mitochondria biogenesis are affected. Results: In this study, we successfully achieved Mstn knockout in skeletal muscle C2C12 cells using a CRISPR/Cas9 system and measured proliferation and differentiation using the Cell-Count...
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#1Sen Ma (NWAFU: Northwest A&F University)H-Index: 3
#2Ying Wang (NWAFU: Northwest A&F University)H-Index: 1
Last. Yulin Chen (NWAFU: Northwest A&F University)H-Index: 15
view all 8 authors...
Dermal papilla cells (DPCs), the “signaling center” of hair follicle (HF), delicately master continual growth of hair in mammals including cashmere, the fine fiber annually produced by secondary HF embedded in cashmere goat skins. Such unparalleled capacity bases on their exquisite character in instructing the cellular activity of hair-forming keratinocytes via secreting numerous molecular signals. Past studies suggested microRNA (miRNAs) and long non-coding RNAs (lncRNAs) play essential roles i...
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