Match!
Dieter Egli
Columbia University
98Publications
34H-index
4,254Citations
Publications 98
Newest
#1Kylie S. Foo (KI: Karolinska Institutet)H-Index: 3
#2Alicja A. Skowronski (Columbia University)H-Index: 6
Last.Dieter Egli (Columbia University)H-Index: 34
view all 10 authors...
Type I diabetes (T1D) is caused by immune-mediated destruction of pancreatic beta cells. This process is triggered, in part, by specific (aa 9–23) epitopes of the insulin Β chain. Previously, fish insulins were used clinically in patients allergic to bovine or porcine insulin. Fish and human insulin differ by two amino acids in the critical immunogenic region (aa 9–23) of the B chain. We hypothesized that β cells synthesizing fish insulin would be less immunogenic in a mouse model of T1D. Transg...
#1Tarun S. Nambiar (Columbia University)
#2Pierre Billon (Columbia University)
Last.Andrew Palacios (Columbia University)
view all 0 authors...
Precise editing of genomic DNA can be achieved upon repair of CRISPR-induced DNA double-stranded breaks (DSBs) by homology-directed repair (HDR). However, the efficiency of this process is limited by DSB repair pathways competing with HDR, such as non-homologous end joining (NHEJ). Here we individually express in human cells 204 open reading frames involved in the DNA damage response (DDR) and determine their impact on CRISPR-mediated HDR. From these studies, we identify RAD18 as a stimulator of...
#1Anthony I. Romer (Columbia University)H-Index: 4
#2Ruth A. Singer (Columbia University)H-Index: 5
Last.Lori Sussel (Columbia University)H-Index: 36
view all 5 authors...
Inactivation of the β cell transcription factor NEUROD1 causes diabetes in mice and humans. In this study, we uncovered novel functions of Neurod1 during murine islet cell development and during the differentiation of human embryonic stem cells (HESCs) into insulin-producing cells. In mice, we determined that Neurod1 is required for perinatal proliferation of alpha and beta cells. Surprisingly, apoptosis only makes a minor contribution to beta cell loss when Neurod1 is deleted. Inactivation of N...
#1Ido Sagi (HUJI: Hebrew University of Jerusalem)H-Index: 7
#2Joao C. De Pinho (Columbia University)H-Index: 1
Last.Thiago Cabral (Columbia University)H-Index: 6
view all 17 authors...
Summary Genomic imprinting is an epigenetic mechanism that results in parent-of-origin monoallelic expression of specific genes, which precludes uniparental development and underlies various diseases. Here, we explored molecular and developmental aspects of imprinting in humans by generating exclusively paternal human androgenetic embryonic stem cells (aESCs) and comparing them with exclusively maternal parthenogenetic ESCs (pESCs) and bi-parental ESCs, establishing a pluripotent cell system of ...
#1Peng Wang (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 6
#2Esra Karakose (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 2
Last.Karen K. Takane (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 3
view all 19 authors...
Summary Small-molecule inhibitors of dual-specificity tyrosine-regulated kinase 1A (DYRK1A) induce human beta cells to proliferate, generating a labeling index of 1.5%–3%. Here, we demonstrate that combined pharmacologic inhibition of DYRK1A and transforming growth factor beta superfamily (TGFβSF)/SMAD signaling generates remarkable further synergistic increases in human beta cell proliferation (average labeling index, 5%–8%, and as high as 15%–18%), and increases in both mouse and human beta ce...
#1Daniela Georgieva (Columbia University)H-Index: 1
#2Qian Liu (Children's Hospital of Philadelphia)H-Index: 1
Last.Dieter Egli (Columbia University)H-Index: 34
view all 4 authors...
DNA synthesis is a fundamental requirement for cell proliferation and DNA repair, but no tools exist to identify the location, direction and speed of replication forks with base pair resolution. Mammalian cells have the ability to incorporate thymidine analogs along with the natural A, T, G and C bases during DNA synthesis, which allows for labelling of replicating or repaired DNA. Most sequencing platforms rely on base-pairing to identify the four canonical nucleotides, and are thus unable to d...
#1Qian Liu (Children's Hospital of Philadelphia)H-Index: 1
#2Daniela Georgieva (Columbia University)H-Index: 1
Last.Kai Wang (UPenn: University of Pennsylvania)H-Index: 55
view all 4 authors...
Recent advances in single-molecule sequencing techniques, such as Nanopore sequencing, improved read length, increased sequencing throughput, and enabled direct detection of DNA modifications through the analysis of raw signals. These DNA modifications include naturally occurring modifications such as DNA methylations, as well as modifications that are introduced by DNA damage or through synthetic modifications to one of the four standard nucleotides. To improve the performance of detecting DNA ...
12345678910